UV-B-Induced Gene Expression in European Beech and Scots Pine

A b s t r a c tChanges in the patterns of gene expression were analyzed in ultraviolet-B (UV-B; 280-320 nm) -treated seedlings of European beech by differential display reverse transcriptase polymerase chain reaction (DDRT-PCR) of mRNA. Utilizing 12 different downstream primers and 27 degenerated 10-mer upstream primers 50 induced and 29 repressed cDNA bands were detected. Nineteen bands were subcloned and sequenced. Two clones revealed significant homologies to known database sequences: putative nascent polypepetide-associated complex alpha and 60S ribosomal protein L37. However, only the latter was confirmed to be UV-B-induced by northern blot analysis.List of abbreviations: CHS = chalcone synthase, DDRT-PCR = differential display reverse transcriptase polymerase chain reaction, EST = expressed sequence tag, PAL = phenylalanine ammonia-lyase, PPFR = photosynthetic photon fluence rate, UV-B = ultraviolet B, 280-320 nm I n t r o d u c t i o n

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Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings

Ernst

Liegl

Kiefer

et al. 2001

Acta Physiol Plant

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UV-B-Induced Gene Expression in European Beech and Scots Pine

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Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings

Early changes in mRNA populations in leaves of ultraviolet-B-treated European beech (Fagus sylvatica L.) seedlings

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