UV-B photoreceptor-mediated signalling in plants

Heijde, Marc; Ulm, Roman

doi:10.1016/j.tplants.2012.01.007

Cited by 393 publications

(294 citation statements)

References 66 publications

Supporting

Mentioning

289

Contrasting

Unclassified

Order By: Relevance

“…g085050 (herein referred to as Cr-COP1; previously also described as PUTATIVE LIGHT RESPONSE SIGNALING PROTEIN1 [LRS1]; Schierenbeck et al, 2015). The identification of Cr-COP1 further suggests the presence of a conserved UV-B signaling pathway similar to the known pathway in Arabidopsis (Heijde and Ulm, 2012;Li et al, 2013;Tilbrook et al, 2013;Jenkins, 2014). Cr-UVR8 and Cr-COP1 cDNAs were cloned and verified to match their respective sequence annotations.…”

Section: Structural Conservation and Uv-b Dynamics Of Chlamydomonas Uvr8mentioning

confidence: 99%

UV-B Perception and Acclimation in Chlamydomonas reinhardtii

et al. 2016

Self Cite

View full text Add to dashboard Cite

Plants perceive UV-B, an intrinsic component of sunlight, via a signaling pathway that is mediated by the photoreceptor UV RESISTANCE LOCUS8 (UVR8) and induces UV-B acclimation. To test whether similar UV-B perception mechanisms exist in the evolutionarily distant green alga Chlamydomonas reinhardtii, we identified Chlamydomonas orthologs of UVR8 and the key signaling factor CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1). Cr-UVR8 shares sequence and structural similarity to Arabidopsis thaliana UVR8, has conserved tryptophan residues for UV-B photoreception, monomerizes upon UV-B exposure, and interacts with Cr-COP1 in a UV-B-dependent manner. Moreover, Cr-UVR8 can interact with At-COP1 and complement the Arabidopsis uvr8 mutant, demonstrating that it is a functional UV-B photoreceptor. Chlamydomonas shows apparent UV-B acclimation in colony survival and photosynthetic efficiency assays. UV-B exposure, at low levels that induce acclimation, led to broad changes in the Chlamydomonas transcriptome, including in genes related to photosynthesis. Impaired UV-B-induced activation in the Cr-COP1 mutant hit1 indicates that UVR8-COP1 signaling induces transcriptome changes in response to UV-B. Also, hit1 mutants are impaired in UV-B acclimation. Chlamydomonas UV-B acclimation preserved the photosystem II core proteins D1 and D2 under UV-B stress, which mitigated UV-B-induced photoinhibition. These findings highlight the early evolution of UVR8 photoreceptor signaling in the green lineage to induce UV-B acclimation and protection.

show abstract

Section: Structural Conservation and Uv-b Dynamics Of Chlamydomonas Uvr8mentioning

confidence: 99%

UV-B Perception and Acclimation in Chlamydomonas reinhardtii

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…UV-B also activates physical defenses, via the UVR8 photoreceptor mediated synthesis of UV-screening pigments, an important aspect reviewed elsewhere [2,14]. 1D) and thus increases the possibility of cellular damage (section 2.1).…”

Section: Figurementioning

confidence: 99%

UV-B effects on leaves—Oxidative stress and acclimation in controlled environments

2016

View full text Add to dashboard Cite

As the steady decline in the Earth's stratospheric ozone layer and parallel increase in solar ultraviolet-B (UV-B: 280-315 nm) has come to an end, the focus of plant UV research has been shifted from regarding UV-B as threatening plant life to recognizing it as a regulatory factor. While UV-B photoreceptor mediated signaling is increasingly understood, the role of UV-B inducible reactive oxygen species is still to be explored. Earlier experiments with high UV-B irradiation doses and isolated thylakoid membranes demonstrated the potential of UV-B to trigger oxidative stress.However, under realistic UV conditions pro-oxidants cannot be reliably traced in more complex biological samples possessing an array of antioxidant defenses. In the absence of direct experimental evidence we must rely on indications and propose hypotheses on how and whether pro-oxidants, such as reactive oxygen species contribute to acclimative responses. Here we briefly review how a balance between pro-oxidants and antioxidants is affected by UV-B in whole plant experiments performed in controlled environments. A working hypothesis is proposed in which the extents of UVinduced peroxidase and superoxide dismutase activations affect the success of acclimation to UV-B.

show abstract

“…UV-B radiation (UV-B; 280 to 315 nm) is a critical regulatory signal that induces photomorphogenic responses in plants (Heijde and Ulm, 2012;Li et al, 2013;Jenkins, 2014). These UV-B-induced responses are mediated by the photoreceptor UV RESISTANCE LOCUS8 (UVR8) in Arabidopsis thaliana (Rizzini et al, 2011) and include hypocotyl growth inhibition (Ballare et al, 1995;Kim et al, 1998;Favory et al, 2009), altered leaf morphogenesis (Hectors et al, 2007;Wargent et al, 2009), stomatal closure (Tossi et al, 2014), and the biosynthesis of UV light-absorbing "sunscreen" compounds (Beggs and Wellmann, 1994;Kliebenstein et al, 2002;Stracke et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

UV-B-Responsive Association of the Arabidopsis bZIP Transcription Factor ELONGATED HYPOCOTYL5 with Target Genes, Including Its Own Promoter

et al. 2014

Self Cite

View full text Add to dashboard Cite

In plants subjected to UV-B radiation, responses are activated that minimize damage caused by UV-B. The bZIP transcription factor ELONGATED HYPOCOTYL5 (HY5) acts downstream of the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8) and promotes UV-B-induced photomorphogenesis and acclimation. Expression of HY5 is induced by UV-B; however, the transcription factor(s) that regulate HY5 transcription in response to UV-B and the impact of UV-B on the association of HY5 with its target promoters are currently unclear. Here, we show that HY5 binding to the promoters of UV-B-responsive genes is enhanced by UV-B in a UVR8-dependent manner in Arabidopsis thaliana. In agreement, overexpression of REPRESSOR OF UV-B PHOTOMORPHOGENESIS2, a negative regulator of UVR8 function, blocks UV-B-responsive HY5 enrichment at target promoters. Moreover, we have identified a T/G-box in the HY5 promoter that is required for its UV-B responsiveness. We show that HY5 and its homolog HYH bind to the T/G HY5 -box cis-acting element and that they act redundantly in the induction of HY5 expression upon UV-B exposure. Therefore, HY5 is enriched at target promoters in response to UV-B in a UVR8 photoreceptor-dependent manner, and HY5 and HYH interact directly with a T/G-box cis-acting element of the HY5 promoter, mediating the transcriptional activation of HY5 in response to UV-B.

show abstract

UV-B photoreceptor-mediated signalling in plants

Cited by 393 publications

References 66 publications

UV-B Perception and Acclimation in Chlamydomonas reinhardtii

UV-B Perception and Acclimation in Chlamydomonas reinhardtii

UV-B effects on leaves—Oxidative stress and acclimation in controlled environments

UV-B-Responsive Association of the Arabidopsis bZIP Transcription Factor ELONGATED HYPOCOTYL5 with Target Genes, Including Its Own Promoter

Contact Info

Product

Resources

About