UV-B radiation enhances isoflavone accumulation and antioxidant capacity of soybean calluses

Abstract

Isoflavones are a class of flavonoids that belong to a large family of polyphenols and synthesized predominantly in legume, and they play important roles including acting as antioxidant, preventing osteoporosis, reducing the risk of atherosclerosis, and protecting against cardiovascular disease. This study focused on the accumulation and synthetic metabolism of isoflavone in soybean hypocotyl and cotyledon calluses under UV-B radiation. The results showed that UV-B radiation significantly up-regulated the gene expression of phenylalanine ammonia lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate-CoA ligase (4CL), chalcone ketone synthase (CHS), chalcone isomerase (CHI), and isoflavone synthase (IFS), and enhanced their activity in soybean hypocotyl and cotyledon calluses. As a result, isoflavones content increased by 21.23 and 21.75% in soybean hypocotyl and cotyledon calluses, respectively. Among the isoflavones produced, malonyldaidzin was the dominant one in hypocotyl callus, while malonylglycitin and daidzein were the main isoflavones in cotyledon calluses. This study revealed that UV-B radiation induced isoflavone accumulation in soybean calluses, which could be an efficient strategy to improve the nutritional value of food and produce high levels of bioactive secondary metabolites.

Melatonin mediates phenolic acids accumulation in barley sprouts under MeJA stress

“…6). Similar results were reported in soybean (33) and barley (24) sprouts. Under stress, plants experience an overabundance of ROS, leading to oxidative harm and the initiation of antioxidative protection mechanisms.…”

“…Our study demonstrated that the exogenous MT significantly reduces the accumulation of H 2 O 2 and MDA under MeJA treatment ( Figures 3A – C , p < 0.05), enhances the ability to scavenge DPPH and ABTS free radicals ( Figures 4E , F , p < 0.05), as well as the fluorescence intensity of H 2 O 2 , and ( Figure 6 ). Similar results were reported in soybean ( 33 ) and barley ( 24 ) sprouts. Under stress, plants experience an overabundance of ROS, leading to oxidative harm and the initiation of antioxidative protection mechanisms.…”

Multiomics Analyses Reveal the Dual Role of Flavonoids in Pigmentation and Abiotic Stress Tolerance of Soybean Seeds

“…The antioxidation property of flavonoids has been evidenced in many other reports. 26,27 Due to the high content level of flavonoids accumulated in the seeds of BLK, we speculated that those antioxidants might help to combat environmental stress during germination. There was no significant germination difference between BLK and YL seeds when they were imbibed in distilled water, implying that the developmental program of seeds was not affected in the BLK.…”