UV-C irradiation is able to inactivate pathogens found in commercially collected porcine plasma as demonstrated by swine bioassay

Blázquez, Elena; Rodrı́guez, Carmen; Ródenas, Jesús; Navarro, Núria; Rosell, Rosa; Pina-Pedrero, Sonia; Campbell, Joy; Sibila, Marina; Segalés, Joaquím; Pujols, Joan; Polo, Javier

doi:10.1016/j.vetmic.2019.108450

Cited by 6 publications

(5 citation statements)

References 22 publications

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“…Papademas et al ( 75 ) tested this device with 7 L of artificially inoculated ( L. innocua , S. aureus , B. cereus , Cronobacter sakazakii , E. coli , Salmonella enteriditis ; 1 × 10 5 CFU/mL) donkey milk and observed a complete inactivation at doses in the range of 200–600 J/L, except for L. innocua who need a dose of 1,100 J/L. By using the same prototype, a series of papers ( 76 , 77 ) of the same group confirmed the capability of Sure Pure Turbulator UV-C treatment system to inactivate significant levels of swine enveloped and non-enveloped viruses as well as bacterial strains ( Salmonella typhimurium , Salmonella choleraesuis , Enterococcus faecium ) inoculated in commercially collected porcine or bovine plasma.…”

Section: Other New Advanced Technologies Of Hm Treatmentmentioning

confidence: 99%

New alternatives to holder pasteurization in processing donor milk in human milk banks

Moro,

Girard,

Peila

et al. 2024

Front. Nutr.

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Infectious and toxicological risks are the main potential hazards that operators of Human Milk Banks (HMBs) encounter and must eliminate. HMBs are trying to implement procedures that allow to manage and sanitize human milk without altering significantly its nutritional and biologically protective components, obtaining a product characterized by a valid balance between safety and biological quality. The history of human milk processing is linked to the origins of HMBs themselves. And although other forms of sterilization were used originally, pasteurization soon became the recognized most effective means for sanitizing milk: all the milk that arrives at the HMB must be pasteurized. Holder pasteurization (HoP) is the most used methodology, and it is performed using low temperature and long time (+62.5°C for 30 min). With HoP some bioactive milk components are lost to varying degrees, but many other precious bioactive compounds are completely or partially preserved. To improve the quality of human milk processed by HMBs, maintaining in the meantime the same microbiological safety offered by HoP, new technologies are under evaluation. At present, High-Temperature Short-Time pasteurization (HTST) and High-Pressure Processing are the most studied methodologies. HTST is already utilized in some HMBs for daily practical activity and for research purposes. They seem to be superior to HoP for a better preservation of some nutritional and biologically protective components. Freeze-drying or lyophilization may have advantages for room temperature storage and transportation. The aim of this study is to evaluate the advancement regarding the processing of DHM with a literature search from 2019 to 2022. The effects of the new technologies on safety and quality of human milk are presented and discussed. The new technologies should assure microbiological safety of the final product at least at the same level as optimized HoP, with an improved preservation of the nutritional and bioactive components of raw human milk.

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Section: Other New Advanced Technologies Of Hm Treatmentmentioning

confidence: 99%

New alternatives to holder pasteurization in processing donor milk in human milk banks

Moro,

Girard,

Peila

et al. 2024

Front. Nutr.

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“…Additionally, the disinfection of biological fluids (plasma and blood products) by photoantimicrobials has been performed for decades and is a well-regarded technological application of these compounds. For instance, extracorporeal photoinactivation of coronaviruses and other clinically relevant pathogens using methylene blue (MB)-mediated aPDT has been reported [ [57] , [58] , [59] , [60] , [61] , [62] ].…”

Section: Photoantimicrobials and Photodynamic Therapymentioning

confidence: 99%

Light-based technologies for management of COVID-19 pandemic crisis

Sabino

Ball

Baptista

et al. 2020

Journal of Photochemistry and Photobiology B: Biology

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The global dissemination of the novel coronavirus disease (COVID-19) has accelerated the need for the implementation of effective antimicrobial strategies to target the causative agent SARS-CoV-2. Light-based technologies have a demonstrable broad range of activity over standard chemotherapeutic antimicrobials and conventional disinfectants, negligible emergence of resistance, and the capability to modulate the host immune response. This perspective article identifies the benefits, challenges, and pitfalls of repurposing light-based strategies to combat the emergence of COVID-19 pandemic.

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“…To validate the effectiveness of the plasma UV-C irradiation measured by means of the viral load reduction in cell culture, a bioassay was done with different groups of pigs injected intraperitoneally with UV-C irradiated commercial liquid plasma at 0 J/L (untreated plasma), 3000 J/L, and at 9000 J/L [77]. The results of the bioassay showed that none of the pigs in the groups that received liquid plasma irradiated by UV-C at either dose became infected or seroconverted against the different virus genomes that were detected in the initial plasma (PCV-2, PRRSV (European strains), SIV, PPV, HEV, Rotavirus A (RVA); thus, confirming the efficacy of UV-C demonstrated in vitro in a previous study [74].…”

Section: Uv Irradiation Effect On Bacteria Survival In Sdpmentioning

confidence: 99%

Biosafety steps in the manufacturing process of spray-dried plasma: a review with emphasis on the use of ultraviolet irradiation as a redundant biosafety procedure

et al. 2020

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Spray dried plasma (SDP) is a functional protein source obtained from blood of healthy animals, approved by the veterinary authorities from animals declared to be fit for slaughter for human consumption. Blood of these animals is collected at the slaughterhouse, treated with an anticoagulant, chilled and transported to industrial facilities in which blood is centrifuged to separate the red blood cells from the plasma fraction. Plasma is then concentrated, and spray dried at high temperatures (80°C throughout its substance) to convert it in a powder. Such method preserves the biological activity of its proteins, mainly albumins and globulins. SDP is mainly used in pig feed diets to significantly improve daily gain, feed intake, production efficiency, and to reduce post-weaning lag caused by the appearance of post-weaning diarrhea. Although SDP is considered a safe product and its manufacturing process consists of several biosafety steps, the security of the SDP is often questioned due to its nature as raw blood by-product, especially when emergent or re-emergent pathogens appear. This review provides an evaluation and validation of the different safety steps present in the manufacturing process of SDP, with special focus on a new redundant pathogen inactivation step, the UV-C irradiation, that may be implemented in the manufacturing process of the SDP. Overall results showed that the manufacturing process of SDP is safe and the UV-C radiation was effective in inactivating a wide range of bacteria and viruses spiked and naturally present in commercially collected liquid animal plasma and it can be implemented as a redundant biosafety step in the manufacturing process of the SDP. Background Spray-dried plasma (SDP) is a functional protein source obtained from blood of healthy animals approved to be sacrificed for human consumption after veterinary inspection. Blood of these animals is collected at the slaughterhouse, treated with an anticoagulant, chilled and transported to industrial facilities where the blood is centrifuged to separate the red blood cells (RBC) from the plasma fraction. Alternatively, the blood may be centrifuged in the abattoir and then the chilled plasma transported to the manufacturing plant. Plasma is subsequently concentrated either by membrane filtration or vacuum evaporation and spray-dried at high temperatures (80°C throughout its substance) to convert it to powder. This process preserves the biological activity of

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UV-C irradiation is able to inactivate pathogens found in commercially collected porcine plasma as demonstrated by swine bioassay

Cited by 6 publications

References 22 publications

New alternatives to holder pasteurization in processing donor milk in human milk banks

New alternatives to holder pasteurization in processing donor milk in human milk banks

Light-based technologies for management of COVID-19 pandemic crisis

Biosafety steps in the manufacturing process of spray-dried plasma: a review with emphasis on the use of ultraviolet irradiation as a redundant biosafety procedure

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