UV Light Assisted Coating Method of Polyphenol Caffeic Acid and Mediated Immobilization of Metallic Silver Particles for Antibacterial Implant Surface Modification

Abstract

Titanium implants are extensively used in biomedical applications due to their excellent biocompatibility, corrosion resistance, and superb mechanical stability. In this work, we present the use of polycaffeic acid (PCA) to immobilize metallic silver on the surface of titanium materials to prevent implant bacterial infection. Caffeic acid is a plant-derived phenolic compound, rich in catechol moieties and it can form functional coatings using alkaline buffers and with UV irradiation. This combination can trigger oxidative polymerization and deposition on the surface of metallic substrates. Using PCA can also give advantages in bone implants in decreasing inflammation by decelerating macrophage and osteoclast activity. Here, chemical and physical properties were investigated using FE-SEM, EDS, XPS, AFM, and contact angle. The in vitro biocompatibility and antibacterial studies show that PCA with metallic silver can inhibit bacterial growth, and proliferation of MC-3T3 cells was observed. Therefore, our results suggest that the introduced approach can be considered as a potential method for functional implant coating application in the orthopedic field.

Polymer Biointerfaces

“…In vitro biocompatibility and antibacterial studies showed that polycaffeic acid with metallic silver can inhibit bacterial growth, while proliferation of MC-3T3 cells was observed. Therefore, the obtained results suggest that the introduced approach can be considered as a potential method for functional implant coating in the orthopedic field [24].…”

An Updated Review on Silver Nanoparticles in Biomedicine

“…Polymer coatings embedded with AgNPs are unharmful materials for normal cells and only act as protective carriers or enhancers for local anti-infective effects, thus inducing or potentiating the antibacterial activity of metallic biomaterials [ 305 , 306 ]. Titanium implants modified with nanosilver-embedded poly(lactic- co -glycolic) acid (PLGA) coatings showed strong bactericidal activity against opportunistic pathogens, together with important osteoinductive potential [ 307 ].…”

Ag-Based Synergistic Antimicrobial Composites. A Critical Review

“…Withal, the concentration of TE/AgNPs required to achieve pronounced antibacterial effect was not toxic to human skin HaCaT cells ( Figure 5 b II). Another interesting example is provided by Lee et al [ 200 ], with polycaffeic acid (PCA) and AgNPs used in synergistic antibacterial combination as a coating of titanium surface for application in orthopedics ( Figure 5 c I). Although the main role of PCA is to, in situ, reduce and immobilize AgNPs by binding to quinone and semiquinone moieties of PCA, it was found that, even with the presence of single PCA coating, antibacterial effect can be acquired.…”

“…Copyright 2019 Elsevier. ( c ) ( I ) Schematics of the process of synthesis of metallic silver (Ag) on the polycaffeic acid (PCA) on etched titanium (Ti) substrate Ti–PCA [ 200 ]; ( II ) graph of inhibition zone for three strains of E. coli , S. aureus and P. aeruginosa for PCA, PCA–Ti and PCA–Ti–Ag [ 201 ]. ( d ) ( I ) Viral inhibition (%) for virus attachment and penetration experiments with the use of 33 nm and 46 nm AgNPs and 13 nm AgNPs and corresponding carriers; ( II ) SEM images in EDS mode of HSV-2 virus incubated with AgNPs of different sizes (13, 33, 46 nm).White arrows point out on the interaction spot of the NPs with the surface of virion; white bars indicate 100 nm; ( III ) kinetics of AgNPs and HSV-2 interaction expressed as % of HSV-2-infected positive controls [ 203 ].…”