UV Raman spectroscopy—A technique for biological and mineralogical in situ planetary studies

Tarcea, N.; Harz, Michaela; Rösch, Petra; Frosch, Torsten; Schmitt, Michael; Thiele, Hans; Hochleitner, Rupert; Popp, Jürgen

doi:10.1016/j.saa.2007.06.051

Cited by 72 publications

(70 citation statements)

References 31 publications

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“…A wide range of different techniques are applicable. Although the resonant excitation in the UV wavelength region probes taxonomic markers, e.g., DNA/RNA vibrations (28,48), the application of visible or NIR Raman excitation wavelengths allows for a phenotypic characterization (20). By applying micro-Raman spectroscopy, even bacterial identification on the single-cell level can be performed (8,15,43,54), and thus elaborate precultivation can be avoided.…”

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confidence: 99%

Raman Spectroscopy as a Potential Tool for Detection of Brucella spp. in Milk

Meisel

Stöckel

Elschner

et al. 2012

Appl Environ Microbiol

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Detection of Brucella, causing brucellosis, is very challenging, since the applied techniques are mostly time-demanding and not standardized. While the common detection system relies on the cultivation of the bacteria, further classical typing up to the biotype level is mostly based on phenotypic or genotypic characteristics. The results of genotyping do not always fit the existing taxonomy, and misidentifications between genetically closely related genera cannot be avoided. This situation gets even worse, when detection from complex matrices, such as milk, is necessary. For these reasons, the availability of a method that allows early and reliable identification of possible Brucella isolates for both clinical and epidemiological reasons would be extremely useful. We evaluated micro-Raman spectroscopy in combination with chemometric analysis to identify Brucella from agar plates and directly from milk: prior to these studies, the samples were inactivated via formaldehyde treatment to ensure a higher working safety. The single-cell Raman spectra of different Brucella, Escherichia, Ochrobactrum, Pseudomonas, and Yersinia spp. were measured to create two independent databases for detection in media and milk. Identification accuracies of 92% for Brucella from medium and 94% for Brucella from milk were obtained while analyzing the single-cell Raman spectra via support vector machine. Even the identification of the other genera yielded sufficient results, with accuracies of >90%. In summary, micro-Raman spectroscopy is a promising alternative for detecting Brucella. The measurements we performed at the single-cell level thus allow fast identification within a few hours without a demanding process for sample preparation.

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confidence: 99%

Raman Spectroscopy as a Potential Tool for Detection of Brucella spp. in Milk

Meisel

Stöckel

Elschner

et al. 2012

Appl Environ Microbiol

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“…Therefore, the information content of the spectra is high, by comparison with that available from Stokes (conventional) Raman scattering and IR spectroscopy. Resonance Raman spectroscopy is useful for investigating molecules such as RNA, DNA, amino acids, proteins (e.g., Storrie-Lombardi et al, 2001;Tarcea et al, 2007), carotenoids (e.g., Marshall et al, 2007b), and chlorophyll, all of which undergo electronic transitions when excited by UV or visible electromagnetic radiation.…”

Section: Raman Phenomenologymentioning

confidence: 99%

“…Using deep UV excitation (e.g., 244 nm), direct investigation of macromolecules such as DNA or proteins becomes possible due to resonant enhancement (e.g., Manoharan et al, 1990;Chadha et al, 1993;Wu et al, 2001;Jarvis and Goodacre, 2004). Storrie-Lombardi et al (2001) and Tarcea et al (2007) showed that Raman excitation wavelengths in the deep UV region are able to selectively enhance Raman signals of proteins and DNA=RNA. The signals can mainly be assigned to DNA bases as well as aromatic amino acids.…”

Section: Accepted Biogenic Raman Spectral Signaturesmentioning

confidence: 99%

Understanding the Application of Raman Spectroscopy to the Detection of Traces of Life

Marshall

Edwards²,

Jehlička³

2010

Astrobiology

167

109

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Investigating carbonaceous microstructures and material in Earth's oldest sedimentary rocks is an essential part of tracing the origins of life on our planet; furthermore, it is important for developing techniques to search for traces of life on other planets, for example, Mars. NASA and ESA are considering the adoption of miniaturized Raman spectrometers for inclusion in suites of analytical instrumentation to be placed on robotic landers on Mars in the near future to search for fossil or extant biomolecules. Recently, Raman spectroscopy has been used to infer a biological origin of putative carbonaceous microfossils in Early Archean rocks. However, it has been demonstrated that the spectral signature obtained from kerogen (of known biological origin) is similar to spectra obtained from many poorly ordered carbonaceous materials that arise through abiotic processes. Yet there is still confusion in the literature as to whether the Raman spectroscopy of carbonaceous materials can indeed delineate a signature of ancient life. Despite the similar nature in spectra, rigorous structural interrogation between the thermal alteration products of biological and nonbiological organic materials has not been undertaken. Therefore, we propose a new way forward by investigating the second derivative, deconvolution, and chemometrics of the carbon first-order spectra to build a database of structural parameters that may yield distinguishable characteristics between biogenic and abiogenic carbonaceous material. To place Raman spectroscopy as a technique to delineate a biological origin for samples in context, we will discuss what is currently accepted as a spectral signature for life; review Raman spectroscopy of carbonaceous material; and provide a historical overview of Raman spectroscopy applied to Archean carbonaceous materials, interpretations of the origin of the ancient carbonaceous material, and a future way forward for Raman spectroscopy.

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“…[1][2][3][4][5][6] The main drawback associated with UV-resonance Raman spectroscopy is photodegradation of the samples during laser exposure, which can pose a serious problem for the observation of actual biological processes, especially when the observation volume cannot be refreshed, as is the case with microscopic imaging. 7,8 In order to develop non-destructive UV-resonance Raman microscopic measurement methods, the origin of UV photodegradation during spectroscopic measurements must be more clearly defined.…”

Section: Introductionmentioning

confidence: 99%