UV-responsive cyclic peptide progelator bioinks

Abstract

We describe the development of cyclic peptide bioinks that self-assemble into gels in response to UV activation.

Probing Thermoresponsive Polymerization-Induced Self-Assembly with Variable-Temperature Liquid-Cell Transmission Electron Microscopy

“…To prove that thermally initiated RAFT polymerization can occur in a controlled manner inside the liquid cell with sufficient exclusion of oxygen, we used matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) to analyze polymers formed in situ (Figure 7). 68,69 Specifically, DMA was polymerized in water with a water-soluble diacid CTA and VA-057 at 70 C inside a VT-LCTEM liquid cell with a target molecular weight of 6,000 Da ( Figure 7A). To ensure oxygen-free conditions for the radical polymerization in the cell, we purged the lines with N 2 and filled them with degassed water before pipetting the polymerization solution onto a bottom LCTEM chip under N 2 flow ( Figure S13).…”

Highlights from Faraday Discussion 301: Nanolithography of Biointerfaces, London, UK, July 3–5 2019

“…This talk was followed by Nathan Gianneschi (Northwestern University), whose group is using cyclic peptides as "biological inks" to create artificial tissues. 9 In this platform, the cyclic peptides are immobilized using a nanoprinter. The macrocyclic constraint of these molecules prevents them from gelling, such that incubation with light and a matrix metalloproteinase enzyme results in the cleavage of the macrocyclic amide bond and converts the molecule into a linear format, such that they can assembled and form hydrogels.…”

“…A photochemical printer uses digital micromirror devices and microfluidics to immobilize alkene-functionalized mannosides in varying densities onto thiol-terminated surfaces. 9 (B) Comparison of the high-density (A') and low density (B') neoglycoprotein microarrays generated by the Gildersleeve laboratory and the Consortium for Functoinal Glycomics microarray (C'). 10 (C) Thermalresponsive GM1os-pentasaccharide bearing glycopolymers collapse above a defined LCST (lower critical solution temperatures) and can be used for the reversible capture of cholera-toxin-B from complex biological mixtures.…”