UV-Spectrophotometric-Assisted Chemometric Methods for the Simultaneous Determination of Metformin Hydrochloride and Gliclazide in Pharmaceutical Formulations

Bhaskar, Radhika; Bhaskar, Rahul; Sagar, Mahendra K.; Saini, Vipin; Bhat, KM

doi:10.4172/2153-2435.1000158

Cited by 8 publications

(5 citation statements)

References 13 publications

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“…According to reports several methods are available for determining OFLOX and TZ quantitatively in combination and as individual compounds, such as UV [3][4][5][6][7][8][9][10][11][12] , HPTLC [13][14][15][16] , and RP-HPLC [17][18] .…”

Section: Introductionmentioning

confidence: 99%

An instantaneous comparison between the chemometrics-assisted spectrophotometric method and liquid chromatography, a technique for identifying antibiotics in their compounded dosage formulations

Jain

Patil

2023

Preprint

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Our study used chemometric-based UV–visible spectrophotometry and Reversed-phase chromatography (habitually stated to as RP chromatography) methods to estimate the amounts of pharmacological dosage forms with a combination of ofloxacin and tinidazole. Quantifying each drug in mixtures was done using UV absorption spectra of 280 to 320 nm and ∆λ of 0.5 nm. For the development of the chemometric model, 24 binary mixture solutions were used, and for validation, twelve solutions were chosen. No prior separation is required for the chemometric-assisted analysis. RP-HPLC units were constructed for quantification by using a THERMOSIL C18 column, a 0.8 and 1.0 ml/min flow rate, and an 85% phosphate: 15% acetonitrile mobile phase. Using these methods, multiple drugs were determined simultaneously in a synthetic mixture. RP-HPLC had a good agreement with PCR and PLS results when compared with the PCR and PLS results.

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Section: Introductionmentioning

confidence: 99%

An instantaneous comparison between the chemometrics-assisted spectrophotometric method and liquid chromatography, a technique for identifying antibiotics in their compounded dosage formulations

Jain

Patil

2023

Preprint

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“…Some authors have studied the application of these methods for analyzing real objects. [1][2][3][4][5][6][7][8] In this paper, we investigate the development of an analysis procedure for simultaneous determination of Paracetamol (PAR) and Codeine phosphate (COP) in Effer-Paralmax® Codeine effervescent tablets that are currently circulating in the Vietnamese market. PAR, with a molecular formula of C 8 H 9 NO 2 , M = 151.17 g/mol, is a metabolite with activity of phenacetin, a pain-relieving and antipyretic medication that is most effective in reducing pain, reducing body temperature in feverish patients by acting on the lower hypothalamus, dissipating heat by dilating blood vessels and increasing peripheral blood flow.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of paracetamol and codeine phosphate in pharmaceuticals using molecular absorption spectroscopy and classical least squares method

Luu¹,

Duy²,

Tran³

2023

MOJABB

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In this article, a combined method of molecular absorption spectroscopy and classical least squares is used to simultaneously determine paracetamol (PAR) and codeine phosphate (COP) in pharmaceutical samples without the need for prior separation or extraction steps. The absorption spectra of standard solutions and samples were measured in the wavelength range of 210 to 290 nm with a step size of 0.5 nm. The concentrations of PAR and COP in the sample solutions were calculated using a self-made program written in Microsoft Excel 2016 and Visual Basic for Applications (VBA). The reliability of the method was verified through the accuracy and reproducibility of the measurements when analyzing PAR and COP in Effer-Paralmax® Codeine tablets, and comparing the average values of their concentrations in the samples with the standard HPLC method.

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“…Metformin hydrochloride has been determined simultaneously with canagliflozin using RP-HPLC [4][5][6][7] and spectrophotometry [8]. In presence of gliclazide, metformin hydrochloride has been determined by High Performance Thin Layer Chromatography (HPTLC) [9,10], Liquid Chromatography-Mass Spectrometry (LC-MS) [11,12], spectrophotometric methods [13][14][15][16][17], and HPLC with Ultra-violet (UV) detection [18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Rp-HPLC and Chemometric Methods for the Determination of Two Anti-Diabetic Mixtures; Metformin Hydrochloride-Canagliflozin and Metformin Hydrochloride-Gliclazide in Their Pharmaceutical Formulation

Al-Bathish

Gazy

El-Jamal

2019

Int J Pharm Pharm Sci

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Objective: To develop and validate novel more sensitive analytical methods for the concurrent quantification of metformin-canagliflozin and metformin-gliclazide in their bulk forms and in their pharmaceutical preparations. Methods: Two methods were developed based on several chemometric assisted spectrophotometric methods and a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC). The first method applies different spectrophotometric chemometric assisted methods, including ratio difference, derivative ratio and extended ratio subtraction method, while the second method describes a RP-HPLC separation of metformin hydrochloride-canagliflozin and metformin hydrochloride-gliclazide binary mixtures using a C18 column with a mobile phase consisting of acetonitrile: potassium dihydrogen phosphate (adjusted to pH 3) with sodium lauryl sulphate as additive in the ratio of 30:70 (%v/v) in isocratic elution mode at 1 ml/min. Results: The proposed methods were able to quantify each of the studied drugs in their binary mixtures with high percentage recoveries in both methods. The spectrophotometric methods were able to quantify each of metformin, canagliflozin and gliclazide in the ranges of 2.0-20.0 μg/ml, 1.5-40.0 μg/ml and 2.0-30.0 μg/ml, respectively. The RP-HPLC method produced well-resolved peaks at a retention time of 3.92, 6.92 and 9.10 min in the concentration ranges of 50.0-300.0 μg/ml, 5.0-50.0 μg/ml and 10.0-100.0 μg/ml for metformin, canagliflozin and gliclazide, respectively. The proposed methods were optimized and validated in accordance to the International Conference of Harmonisation (ICH) guidelines in terms of linearity, LOD, LOQ, precision and accuracy. Conclusion: The developed methods were found to be sensitive and reproducible methods for the simultaneous determination of anti-diabetic binary mixtures; metformin hydrochloride-canagliflozin and metformin hydrochloride-gliclazide. And thus were successfully employed for the quality control analysis of the pharmaceutical formulations of the studied binary mixtures.

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UV-Spectrophotometric-Assisted Chemometric Methods for the Simultaneous Determination of Metformin Hydrochloride and Gliclazide in Pharmaceutical Formulations

Cited by 8 publications

References 13 publications

An instantaneous comparison between the chemometrics-assisted spectrophotometric method and liquid chromatography, a technique for identifying antibiotics in their compounded dosage formulations

An instantaneous comparison between the chemometrics-assisted spectrophotometric method and liquid chromatography, a technique for identifying antibiotics in their compounded dosage formulations

Simultaneous determination of paracetamol and codeine phosphate in pharmaceuticals using molecular absorption spectroscopy and classical least squares method

Rp-HPLC and Chemometric Methods for the Determination of Two Anti-Diabetic Mixtures; Metformin Hydrochloride-Canagliflozin and Metformin Hydrochloride-Gliclazide in Their Pharmaceutical Formulation

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