UV-Spectrophotometry Determination of Taurine in Energy Drink Mixtures

Draganov, Georgi B.

doi:10.11648/j.ijnfs.20140302.26

Cited by 12 publications

(6 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is conjugated in the liver with chenodeoxycholic acid and cholic acid and form the bile salts sodium taurochenodeoxycholate and sodium taurocholate [34]. Taurine is involved in the formation of the surface-active substances, which promote the emulsification of the fatty acids in the diet [14].…”

Section: Pharmacological Effects Of Taurinementioning

confidence: 99%

Investigation of Some Pharmacological Effects of Caffeine and Taurine in Food Supplements

Tsvetkova¹,

Klisurov²,

Pankova³

et al. 2015

IJNFS

View full text Add to dashboard Cite

Abstract:In modern times in all age groups energy supplements containing different amounts of Caffeine and Taurine are applied. Caffeine is purine alkaloid, which stimulates central nervous system action, enhances the strength and frequency of the cardiac contractions and increases the excretion of urine. Taurine is a sulfur containing amino acid, which possesses many fundamental biological roles including: effect on synaptic transmission in the central nervous system, cardiotropic action, antioxidant and anticonvulsant activity, improvement of energy processes, stimulation of reparative processes in tissues, protection of eyes cataract, decrease of cholesterol and stimulation of immune system. The combination of Caffeine and Taurine provide benefit due to obtaining synergism of pharmacological effects in increasing of physical activity, stimulation of brain action, cognition, memory and attention. In connection with the significant enlarging of the consumption of energy drinks, especially by children and young people in recent years the requirements for regulation and control of the labeling of these products in many countries are enlarged. In many food additives Caffeine and Taurine are added in not labeled high concentrations, which can provoke and increase their side effects. High consumption of Caffeine enhances its adverse effects on body: anxiety, headache, insomnia, nervousness, respiratory disorders, tachycardia, tremor, dehydration. In children the adverse reactions of Caffeine in much lower doses than adults are occurred. In high concentrations Taurine has adverse effects on brain activity and can induce psoriasis. The result of combination of Caffeine and Taurine is associated with increased diuretic effect and loss of water and salts from the body, especially in children and young people. Because of these facts the quality and quantity control of included compounds in food supplements is important for their health safety.

show abstract

Section: Pharmacological Effects Of Taurinementioning

confidence: 99%

Investigation of Some Pharmacological Effects of Caffeine and Taurine in Food Supplements

Tsvetkova¹,

Klisurov²,

Pankova³

et al. 2015

IJNFS

View full text Add to dashboard Cite

show abstract

“…It has physiological effects and pharmacological actions such as a neurotransmitter, antioxidant, modulator of intracellular calcium levels, osmolyte, protect the liver and benefit the gallbladder [5]. The various physiological functions of taurine are explained with amino terminal group in the structure and sulfonic acid group moiety [6]. It is added to energy drinks maybe due to its purposed stimulant effects and it may improve athletic performance, improve attention and verbal reasoning skills [7,4].…”

Section: Introductionmentioning

confidence: 99%

Liquid Chromatographic and Spectrophotometric Determination of Taurine in Energy Drinks Based on O-Phthalaldehyde-Sulfite Derivatization

Omer¹,

Omar²,

Abdelaziz³

et al. 2019

J Food Chem Nanotechol

View full text Add to dashboard Cite

Rapid and efficient high-performance liquid chromatographic and UV-Vis spectrophotometric methods have been optimized and validated for taurine determination in energy drinks. Taurine was derivatized with o-phthalaldehyde and sodium sulfite in alkaline media prior to analysis. The optimum derivatization parameters were found to be 0.1 M borate buffer at pH 9.5, reaction time 5.0 min, o-phthalaldehyde concentration of 60 mg L-1 , sodium sulfite concentration of 202 mg L-1 and water as diluting solvent. The analytical parameters such as linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy were investigated. The methods were linear in range 0.5-20 mg L-1 and 0.5-15 mg L-1 with correlation coefficient (r 2) of 0.9998 and 0.9996 for HPLC-PDA and spectrophotometer respectively. The LODs were 0.109 mg L-1 and 0.141 mg L-1 for HPLC-PDA and spectrophotometer respectively. The precision (RSD%) of intra-day and inter-day of the methods were 1.816-1.278% and 2.858-2.236% respectively, for HPLC-PDA and spectrophotometer respectively. Recoveries of taurine ranging from 90% to 105%, (n = 3) were obtained. The methods were successfully applied for determination of taurine in energy drink samples.

show abstract

“…Taurine has several essential physiological functions such as; anti-inflammatory properties, formation of bile salts, acts as an antioxidant, membrane stabilization, and osmosis, liver detoxication by binding harmful substances, protection against glutamate cytotoxicity, neurotransmitter and a modulator of intracellular ions levels [8,9]. Studies have shown that high concentration of taurine can induce side effects such as; declining heart rates while arterial blood pressure is increased (with caffeine), hypoglycemia, dizziness, diarrhea, peptic ulcer, influence defects in nerve blood flow and dehydration [6,10]. The combination of taurine with caffeine, amino acids and guarana in energy drinks increases the risk of side effects [6].…”

Section: Introductionmentioning

confidence: 99%

“…HPLC with electrochemical, refractive index detection and mass spectrometry [7,11] were also reported. Methods using techniques such as capillary electrophoresis [12], nuclear magnetic resonance spectroscopy (H1-NMR) [13] , UVspectrophotometric methods [10,14] and spectrofluorimetric [15] were also found in the literature. However, most of these methods have drawbacks, such as they require expensive equipment and derivatizing reagent [7,9] or have low sensitivity [12,14].…”

Section: Introductionmentioning

confidence: 99%

High Performance Liquid Chromatographic Methods for Analysis of Taurine in Energy Drinks after Pre-column Derivatization

Omer¹,

Omar²,

Thiel³

et al. 2018

Eurasian J Anal Chem

View full text Add to dashboard Cite

Simple and efficient high performance liquid chromatography (HPLC) with photodiode array (PDA) and fluorescence (FLD) detection methods have been validated for determination of taurine in energy drinks. These methods are based on pre-column derivatization of taurine with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) at alkaline medium to form colored fluorescent product. In the both validated HPLC methods, the derivatization product is separated on Intersil ODS-3 analytical column with acetonitrile and 0.1% trichloroacetic acid (30:70, v:v) as mobile phase. The eluted derivative is detected at 472 nm for HPLC-PDA and 472 nm/530 nm (Ex/Em) for HPLC-FLD. The methods were validated in terms of linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy (recovery). Good linearities were achieved for taurine (r 2 > 0.9998 and 0.9993) in the concentration range of 5-50 mg L-1 and 5-50 µg L-1 for HPLC-PDA and HPLC-FLD respectively. The LODs were 0.296 and 0.616 × 10-3 mg L-1 for HPLC-PDA and HPLC-FLD respectively. The precision for peak area were 0.78 and 0.61% for HPLC-PDA and HPLC-FLD respectively. Recoveries of taurine ranging from 92-103.3%), (n = 3) were obtained. The validated method was successfully applied for the determination of taurine in some selected energy drinks available in local markets.

show abstract

UV-Spectrophotometry Determination of Taurine in Energy Drink Mixtures

Cited by 12 publications

References 11 publications

Investigation of Some Pharmacological Effects of Caffeine and Taurine in Food Supplements

Investigation of Some Pharmacological Effects of Caffeine and Taurine in Food Supplements

Liquid Chromatographic and Spectrophotometric Determination of Taurine in Energy Drinks Based on O-Phthalaldehyde-Sulfite Derivatization

High Performance Liquid Chromatographic Methods for Analysis of Taurine in Energy Drinks after Pre-column Derivatization

Contact Info

Product

Resources

About