2020
DOI: 10.1021/acs.jpcb.0c01693
|View full text |Cite
|
Sign up to set email alerts
|

UV–vis Photodissociation Action Spectroscopy Reveals Cytosine–Guanine Hydrogen Transfer in DNA Tetranucleotide Cation Radicals upon One-Electron Reduction

Abstract: We report the generation and spectroscopic study of hydrogen-rich DNA tetranucleotide cation radicals (GATC+2H) +• and (AGTC+2H) +• . The radicals were generated in the gas phase by one-electron reduction of the respective dications (GATC +2H) 2+ and (AGTC+2H) 2+ and characterized by collision-induced dissociation and photodissociation tandem mass spectrometry and UV−vis photodissociation action spectroscopy. Among several absorption bands observed for (GATC+2H) +• , the bands at 340 and 450 nm were assigned t… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
2

Citation Types

2
18
0

Year Published

2020
2020
2024
2024

Publication Types

Select...
6

Relationship

5
1

Authors

Journals

citations
Cited by 11 publications
(20 citation statements)
references
References 63 publications
2
18
0
Order By: Relevance
“…Ion action spectroscopy relies on photodissociation of mass-selected gas-phase ions whereby the wavelength-dependent photofragment ion intensities are used to reconstruct the absorption profile of the precursor ion. Several variants of action spectroscopies have been developed working in the infrared or UV–vis regions of the spectrum. Multiphoton infrared photodissociation spectroscopy has been the most popular technique for small ions, whereas its applications to strongly hydrogen bonded systems have faced difficulties with band assignment and spectral interpretation. , UV–vis spectroscopy utilizing valence electron excitation and single-photon dissociation largely avoids such issues and has been shown to provide structural information for larger biomolecular ions that show characteristic absorption bands, such as peptide and oligonucleotide cation radicals. …”
Section: Introductionmentioning
confidence: 99%
“…Ion action spectroscopy relies on photodissociation of mass-selected gas-phase ions whereby the wavelength-dependent photofragment ion intensities are used to reconstruct the absorption profile of the precursor ion. Several variants of action spectroscopies have been developed working in the infrared or UV–vis regions of the spectrum. Multiphoton infrared photodissociation spectroscopy has been the most popular technique for small ions, whereas its applications to strongly hydrogen bonded systems have faced difficulties with band assignment and spectral interpretation. , UV–vis spectroscopy utilizing valence electron excitation and single-photon dissociation largely avoids such issues and has been shown to provide structural information for larger biomolecular ions that show characteristic absorption bands, such as peptide and oligonucleotide cation radicals. …”
Section: Introductionmentioning
confidence: 99%
“…The conformational changes upon reduction were most dramatic in dinucleotides whose fully extended dication structures buckled about the phosphodiester hinge upon reduction, forming transient folded cation radical structures that underwent proton transfer between the nucleobases. Interactions between adenine and guanine ions and radicals, resulting in backbone refolding and proton transfer, have also been detected for tetranucleotide cation radicals (dGATC + 2H) +• and (dGATT + 2H) +• where the ion structures were assigned on the basis of UV–visPD action spectroscopy and BOMD, DFT, and TD-DFT vibronic calculations. , …”
Section: Reductive Pathways To Cation Radicals In the Gas Phasementioning
confidence: 98%
“…The reductive generation of nucleoside radicals has been applied to gas-phase DNA dinucleotides and tetranucleotides, , as well as RNA dinucleotide chimeras that have been characterized by mass spectrometry, UV–visPD action spectroscopy, Born–Oppenheimer molecular dynamics, and DFT calculations of structure and electronic excitations. The DNA oligonucleotides have been found to undergo substantial conformational changes upon one-electron reduction of their dications.…”
Section: Reductive Pathways To Cation Radicals In the Gas Phasementioning
confidence: 99%
See 2 more Smart Citations