UV‐Visible Spectroscopy for On‐Line Analysis

Liauw, Marcel; Baylor, L. C.; O'Rourke, Patrick

doi:10.1002/9780470689592.ch4

Cited by 14 publications

(10 citation statements)

References 40 publications

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“…A review of the pharmaceutical applications of terahertz pulsed spectroscopy and imaging discussed measuring film-coat thickness and providing 3D images of solid dose products. 18 A nonexhaustive list of other PAT process analyzers include UV-visible spectroscopy, 19 acoustic resonance spectroscopy, 20,21 laser-induced breakdown spectroscopy, 22 acoustic emission, 23 microwave resonance sensors, 24 and process nuclear magnetic resonance spectroscopy. 25 Light-induced fluorescence (LIF) is an underutilized PAT.…”

Section: Introductionmentioning

confidence: 99%

Effect of Tapped Density, Compacted Density, and Drug Concentration on Light-Induced Fluorescence Response as a Process Analytical Tool

Shah

Ely

Stagner

2015

Journal of Pharmaceutical Sciences

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Section: Introductionmentioning

confidence: 99%

Effect of Tapped Density, Compacted Density, and Drug Concentration on Light-Induced Fluorescence Response as a Process Analytical Tool

Shah

Ely

Stagner

2015

Journal of Pharmaceutical Sciences

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“…These spectra can provide poor information, but the utilization of multivariate data analysis has enabled to extract valuable information from them . Up to date, in microorganism fermentation processes and animal cell cultures, UV–vis monitoring has not found wide application . In this work, sample spectra were determined with cell removal for avoiding light scattering effects.…”

Section: Discussionmentioning

confidence: 99%

A multivariate calibration procedure for UV/VIS spectrometric monitoring of BHK‐21 cell metabolism and growth

Leme

Núñez

Parizotto

et al. 2013

Biotechnology Progress

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Monitoring mammalian cell culture with UV–vis spectroscopy has not been widely explored. The aim of this work was to calibrate Partial Least Squares (PLS) models from off-line UV–vis spectral data in order to predict some nutrients and metabolites, as well as viable cell concentrations for mammalian cell bioprocess using phenol red in culture medium. The BHK-21 cell line was used as a mammalian cell model. Spectra of samples taken from batches performed at different dissolved oxygen concentrations (10, 30, 50, and 70% air saturation), in two bioreactor configurations and with two strategies to control pH were used to calibrate and validate PLS models. Glutamine, glutamate, glucose, and lactate concentrations were suitably predicted by means of this strategy. Especially for glutamine and glucose concentrations, the prediction error averages were lower than 0.5060.10 mM and 2.2160.16 mM, respectively. These values are comparable with those previously reported using near infrared and Raman spectroscopy in conjunction with PLS. However, viable cell concentration models need to be improved. The present work allows for UV–vis at-line sensor development, decrease cost related to nutrients and metabolite quantifications and establishment of fed-batch feeding schemes.

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“…Some of these include: UV/visible spectroscopy : This is mainly employed to facilitate in situ sampling of a process with advanced fiber‐optic probes. At the same time, the detection equipment can be aligned far from the process hazards NIR spectroscopy : For measurements of solids, liquids, gases, and supercritical fluids.…”

Section: Integration Of Pat With Traditional Drug Manufacturingmentioning

confidence: 99%