UVA and UVB Irradiation Differentially Regulate microRNA Expression in Human Primary Keratinocytes

Kraemer, Anne; Chen, I-Peng; Henning, Stefan; Faust, Alexandra; Volkmer, Beate; Atkinson, Michael J.; Moertl, Simone; Greinert, Ruediger

doi:10.1371/journal.pone.0083392

Cited by 49 publications

(41 citation statements)

References 73 publications

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“…However, more work is needed to unravel the exact mechanisms that lead to PP. Recent studies have indicated that UVA and UVB irradiation differentially regulates microRNA expression in human keratinocytes as well as in the dermis . MicroRNAs are a group of small non‐coding RNAs that act as post‐transcriptional regulators by binding to complementary sequences in the 3′‐untranslated region of target genes.…”

Section: Perspectivesmentioning

confidence: 99%

Desired response to phototherapy vs photoaggravation in psoriasis: what makes the difference?

Wolf

Weger

Patra

et al. 2016

Experimental Dermatology

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Psoriasis commonly responds beneficially to UV radiation from natural sunlight or artificial sources. Therapeutic mechanisms include the proapoptotic and immunomodulating effects of UV, affecting many cells and involving a variety of pro-and anti-inflammatory cytokines, downregulating the Th17/IL-23 response with simultaneous induction of regulatory immune cells. However, exposure to UV radiation in a subset of psoriasis patients leads to exacerbation of the disease. We herein shed light on the predisposing factors of photosensitive psoriasis, including genetics (such as HLACw*0602 or CARD14), gender and coexisting photodermatoses such as polymorphic light eruption (PLE) in the context of potential molecular mechanisms behind therapeutic photoresponsiveness or photoaggravation. UV-induced damage/pathogenassociated molecular patterns, damage to self-coding RNA (signalling through Toll-like receptors), certain antimicrobial peptides and/or inflammasome activation may induce innate immunity, leading to psoriasis at the site of UV exposure when there is concomitant, predisposing resistance against UV-induced suppression of the adaptive immune response (like in PLE) that otherwise would act to reduce psoriasis. K E Y W O R D S

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Section: Perspectivesmentioning

confidence: 99%

Desired response to phototherapy vs photoaggravation in psoriasis: what makes the difference?

Wolf

Weger

Patra

et al. 2016

Experimental Dermatology

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“…The reduced DNA methylation can alter the expression of mRNAs and miRNAs. Indeed, alteration of miRNAs has been found in cells exposed to UV (119). …”

Section: Environmental and Hormonal Regulation Of Mirnas In Lupusmentioning

confidence: 99%

MicroRNAs in lupus

2014

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Systemic lupus erythematosus (SLE) is a prototypic autoimmune disease characterized by the production of an array of pathogenic autoantibodies, including high-affinity anti-dsDNA IgG antibodies, which plays an important role in disease development and progression. Lupus preferentially affects women during their reproductive years. The pathogenesis of lupus is contributed by both genetic factors and epigenetic modifications that arise from exposure to the environment. Epigenetic marks, including DNA methylation, histone post-translational modifications and microRNAs (miRNAs), interact with genetic programs to regulate immune responses. Epigenetic modifications influence gene expression and modulate B cell functions, such as class switch DNA recombination (CSR), somatic hypermutation (SHM) and plasma cell differentiation, thereby informing the antibody response. Epigenetic dysregulation can result in aberrant antibody responses to exogenous antigens or self-antigens, such as chromatin, histones and dsDNA in lupus. miRNAs play key roles in the post-transcriptional regulation of most gene-regulatory pathways and regulate both the innate and the adaptive immune responses. In mice, dysregulation of miRNAs leads to aberrant immune responses and development of systemic autoimmunity. Altered miRNA expression has been reported in human autoimmune diseases, including lupus. The dysregulation of miRNAs in lupus could be the result of multiple environmental factors, such as sex hormones and viral or bacterial infection. Modulation of miRNA is a potential therapeutic strategy for lupus.

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“…The keratinocytes that are located at the surface of the skin are usually affected by both UVA and UVB when a solar simulator is used. However, each wavelength band may affect the cells differently, generating distinct mechanistic pathways . Here, a narrowband light source was used to evaluate the effects of UVA without the influence of UVB, as performed by other groups .…”

Section: Resultsmentioning

confidence: 99%

Combination of Natural Extracts and Photobiomodulation in Keratinocytes Subjected to UVA Radiation

Silva

Machado

Lino-dos-Santos-Franco

et al. 2018

Photochem & Photobiology

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Natural extracts (NE) with antioxidant properties can minimize the effects of photoaging. Photobiomodulation (PBM) has proven to be a useful tool for the modulation of cell metabolism. Here, we investigate the associations of antioxidants with PBM with the aim of promoting skin rejuvenation. We began with standardization of the experimental protocol. Extracts of chamomile, rosemary, blueberry, green tea, figs, pomegranate and nutwood were tested. A custom irradiation system (366 ± 10 nm) was used to simulate sun exposure. A light emitting diode system (640 ± 12.5 nm) was used for PBM. Viability assessments were performed by the (3‐(4,5‐Dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide) MTT assay method. Based on the results, radiant exposure to UVA was defined as 9 and 1 J cm−2 for PBM. Extract concentrations were established on the basis of dark toxicities, which ranged from 0.01% to 0.3%. The data show that PBM is a promising therapy to restore keratinocytes after UVA damage; however, the detailed mechanism and effects require further exploration. Moreover, although the combination of PBM with NE may be a useful strategy, the choice of a NE is challenging, since the working concentration and other properties, such as photosensitivity, may bring about unwanted results.

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UVA and UVB Irradiation Differentially Regulate microRNA Expression in Human Primary Keratinocytes

Cited by 49 publications

References 73 publications

Desired response to phototherapy vs photoaggravation in psoriasis: what makes the difference?

Desired response to phototherapy vs photoaggravation in psoriasis: what makes the difference?

MicroRNAs in lupus

Combination of Natural Extracts and Photobiomodulation in Keratinocytes Subjected to UVA Radiation

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