UvrA and UvrC subunits of the <i>Mycobacterium tuberculosis</i> UvrABC excinuclease interact independently of UvrB and DNA

Thakur, Manoj; Badugu, Sugith Babu; Muniyappa, K.

doi:10.1002/1873-3468.13671

Cited by 14 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The functions of individual subunits of the E. coli UvrABC complex have been studied in greater detail using genetic, biochemical and structural approaches [1,11,12]. However, emerging evidence supports the notion that the catalytic and mechanistic features of the E. coli UvrABC repair complex are far from being universal in bacteria [1,11,12,37,53,58]. For example, M. tuberculosis is evolutionarily distant from E. coli and that only 41% of M. tuberculosis proteins have clear E. coli homologs [59], and we found that the overall sequence identity between MtUvrC and EcUvrC is 35.5%.…”

Section: Discussionsupporting

confidence: 54%

See 1 more Smart Citation

The intrinsic ATPase activity of Mycobacterium tuberculosis UvrC is crucial for its damage‐specific DNA incision function

2020

Self Cite

View full text Add to dashboard Cite

The nucleotide excision repair pathway is essential for the removal of bulky DNA adducts generated by exogenous chemicals, UV radiation and by‐products of cellular metabolism. In bacteria, the UvrAB complex recognizes and binds to the DNA adducts, and UvrC catalyses the dual incision of the damaged strand. Here, we reveal that Mycobacterium tuberculosis UvrC possesses an intrinsic ATPase activity, which is essential for the incision of the damaged DNA strand.

show abstract

Section: Discussionsupporting

confidence: 54%

“…The K d values of MtUvrC for these substrates are in the same range as that reported for EcUvrC‐CTD [46]. Although firm evidence is lacking, it is likely that a dimeric form of MtUvrC binds to the DNA lesion site [58].…”

Section: Discussionmentioning

confidence: 56%

The intrinsic ATPase activity of Mycobacterium tuberculosis UvrC is crucial for its damage‐specific DNA incision function

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, codon selection is common in both prokaryotes and eukaryotes and fundamentally influences the synthesis of a particular polypeptide and/or the accuracy of translation [ 67 ]. UvrABC excinuclease plays an important role in bacterial nucleotide excision repair [ 68 ]. In the present study, a non-syn mutation was found in the open reading frame of the excinuclease ABC subunit uvrB gene (Cthe_0309), where amino acid His was replaced by Arg at position 402 ( Table S10 ).…”

Section: Resultsmentioning

confidence: 99%

Utilization of Monosaccharides by Hungateiclostridium thermocellum ATCC 27405 through Adaptive Evolution

Ha-Tran

Nguyen

et al. 2021

Microorganisms

View full text Add to dashboard Cite

Hungateiclostridium thermocellum ATCC 27405 is a promising bacterium for consolidated bioprocessing with a robust ability to degrade lignocellulosic biomass through a multienzyme cellulosomal complex. The bacterium uses the released cellodextrins, glucose polymers of different lengths, as its primary carbon source and energy. In contrast, the bacterium exhibits poor growth on monosaccharides such as fructose and glucose. This phenomenon raises many important questions concerning its glycolytic pathways and sugar transport systems. Until now, the detailed mechanisms of H. thermocellum adaptation to growth on hexose sugars have been relatively poorly explored. In this study, adaptive laboratory evolution was applied to train the bacterium in hexose sugars-based media, and genome resequencing was used to detect the genes that got mutated during adaptation period. RNA-seq data of the first culture growing on either fructose or glucose revealed that several glycolytic genes in the Embden–Mayerhof–Parnas pathway were expressed at lower levels in these cells than in cellobiose-grown cells. After seven consecutive transfer events on fructose and glucose (~42 generations for fructose-adapted cells and ~40 generations for glucose-adapted cells), several genes in the EMP glycolysis of the evolved strains increased the levels of mRNA expression, accompanied by a faster growth, a greater biomass yield, a higher ethanol titer than those in their parent strains. Genomic screening also revealed several mutation events in the genomes of the evolved strains, especially in those responsible for sugar transport and central carbon metabolism. Consequently, these genes could be applied as potential targets for further metabolic engineering to improve this bacterium for bio-industrial usage.

show abstract

“…In addition, codon selection is common in both prokaryotes and eukaryotes and fundamentally influences the synthesis of a particular polypeptide and/or the accuracy of translation [69]. UvrABC excinuclease plays an important role in bacterial nucleotide excision repair [70]. In the present study, a Nsy mutation was found in the open reading frame of the excinuclease ABC subunit UvrB gene (Cthe_0309), where His was replaced by Arg at amino acid 402 (Table S6).…”

Section: Genomic Analysismentioning

confidence: 69%

Utilization of Monosaccharides by <em>Hungateiclostridium thermocellum</em> ATCC 27405 Through Adaptive Evolution

Ha-Tran¹,

Nguyen²,

Lo³

et al. 2020

Preprint

View full text Add to dashboard Cite

Hungateiclostridium thermocellum ATCC 27405 is a promising bacterium with a robust ability to degrade lignocellulosic biomass complexes, including crystalline cellulose components, through a multienzyme cellulosomal system. In contrast, it exhibits poor growth on simple monosaccharides such as fructose and glucose. This phenomenon raises many important questions concerning its glycolytic pathways and sugar transport systems. Until now, the detailed mechanisms of H. thermocellum adaptation to growth on monosaccharides have been poorly explored. In this study, adaptive laboratory evolution was applied to train the bacterium on monosaccharides, and genome resequencing was used to detect the genes that had mutated during adaptation. RNA-seq data of the 1st-generation culture growing on either fructose or glucose revealed that several glycolytic genes in the EMP pathway were expressed at lower levels in these cells than in cellobiose-grown cells. After 8 generations of culture on fructose and glucose, the evolved H. thermocellum strains grew faster and yielded greater biomass than the nonadapted strains. Genomic screening also revealed several mutation events in the genomes of the evolved strains, especially in genes responsible for sugar transport and central carbon metabolism. Consequently, these genes could be applied as targets for further metabolic engineering to improve this bacterium for bioindustrial usage.

show abstract

UvrA and UvrC subunits of the Mycobacterium tuberculosis UvrABC excinuclease interact independently of UvrB and DNA

Cited by 14 publications

References 36 publications

The intrinsic ATPase activity of Mycobacterium tuberculosis UvrC is crucial for its damage‐specific DNA incision function

The intrinsic ATPase activity of Mycobacterium tuberculosis UvrC is crucial for its damage‐specific DNA incision function

Utilization of Monosaccharides by Hungateiclostridium thermocellum ATCC 27405 through Adaptive Evolution

Utilization of Monosaccharides by <em>Hungateiclostridium thermocellum</em> ATCC 27405 Through Adaptive Evolution

Contact Info

Product

Resources

About