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Tortoli, Enrico; Piersimoni, Claudio; Bartoloni, Alessandro; Burrini, C; Callegaro, Annapaola; Caroli, G; Colombrita, D.; Goglio, Antonio; Mantella, Antonia; Tosi, C; Simonetti, M. T.

doi:10.1023/a:1007375114106

Cited by 13 publications

(2 citation statements)

References 24 publications

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“…Interestingly, the sequence of the hypervariable region A from our type strain was identical to the "genotype 3" profile of a recently published study on a few mycobacterial isolates phylogenetically related to M. simiae (31). The authors of that study concluded that the genotype 3 isolates (n ϭ 2) had an HPLC phenotype similar to that of M. lentiflavum, but conventional tests identified them as M. simiae.…”

Section: Discussionsupporting

confidence: 73%

Characterization of a Novel Group of Mycobacteria and Proposal of Mycobacterium sherrisii sp. nov

Selvarangan¹,

Wu²,

Nguyen³

et al. 2004

J Clin Microbiol

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We describe here the characterization of five isolates of Mycobacterium simiae-like organisms representing a novel group based on whole-cell fatty acid analysis and genotypic evaluation. Two of the five isolates in this study, W55 and W58, were previously considered to belong to M. simiae serotype 2. Analysis of cellular fatty acids by gas-liquid chromatography indicated a close clustering of this group, which was well differentiated from the other M. simiae-like species. Molecular characterization was performed by nucleic acid sequencing of the small subunit rRNA gene and the gene encoding the 65-kDa heat shock protein and genomic DNA hybridization. Sequence analysis of the entire 16S rRNA gene showed a unique sequence most closely related to those of M. triplex and M. simiae. The hsp65 partial gene sequence was identical for the five isolates, with 97% identity to the M. simiae type strain. However, qualitative whole genomic DNA hybridization analysis confirmed that this group is genetically distinct from M. simiae and M. triplex. Antimicrobial susceptibilities for this group resemble those of M. simiae and M. lentiflavum. We conclude that this group represents a unique Mycobacterium species for which we propose the name Mycobacterium sherrisii sp. nov.The genus Mycobacterium consists of a diverse group of acidfast bacilli that exist in the environment and cause infections in humans and animals (22). Conventional methods for identification of these organisms are well established and inexpensive, but these methods are also time-consuming and labor-intensive and can provide inconsistent results, leading to species identifications based on a "best-fit" method. The introduction of several molecular tools and improved techniques in the last two decades has not only revolutionized the identification process by reducing the time to identification but also has provided clues to the presence of novel strains. Mycolate analysis has proven to be a powerful phenotypic method for determining the presence of new species based on novel patterns (9, 25). Fatty acid analysis techniques such as high-performance liquid chromatography (HPLC) and gas-liquid chromatography (GLC) were developed to analyze the mycolic acid and whole-cell fatty acid profiles, respectively, to identify characteristic patterns of established mycobacterial species (7,10,12,30).The advent of nucleotide probes designed to hybridize to specific rRNA gene sequences allowed rapid identification of the most common mycobacterial species (16). Lately, genotypic analyses aimed at identifying nucleotide sequences of several chromosomal genes in the Mycobacterium genus coding for 16S rRNA, internal transcribed spacer 1, and 65-kDa heat shock protein (hsp65) have proven to be useful in the molecular characterization of well-established species and in identifying new ones, including previously misidentified groups (8,15,17,26). Analysis of the hypervariable region in the 16S rRNA gene sequence has a high species specificity and also has been useful to assign new spec...

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Section: Discussionsupporting

confidence: 73%

Characterization of a Novel Group of Mycobacteria and Proposal of Mycobacterium sherrisii sp. nov

Selvarangan¹,

Wu²,

Nguyen³

et al. 2004

J Clin Microbiol

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“…In contrast to growth behaviour in culture, the sequence pattern was characterized by the occurrence of a short helix 18 within the hypervariable B region of the 16S rRNA gene, which is a molecular signature of fast-growing mycobacteria. These features are shared by a group of micro-organisms that includes M. simiae and other emerging mycobacterial species (Böttger et al, 1993;Kirschner et al, 1993;Meier et al, 1993;Springer et al, 1993Springer et al, , 1996Floyd et al, 1996Floyd et al, , 2000Haas et al, 1997;Tortoli et al, 1997;Herbst et al, 2001). xenopi.…”

mentioning

confidence: 99%

Mycobacterium parmense sp. nov.

Fanti

Tortoli²,

Hall

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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The isolation and identification of a novel, slow-growing, scotochromogenic, mycobacterial species is reported. A strain, designated MUP 1182 T , was isolated from a cervical lymph node of a 3-year-old child. MUP 1182 T is alcohol-and acid-fast, with a lipid pattern that is consistent with those of species that belong to the genus Mycobacterium. It grows slowly at 25-37 6C, but does not grow at 42 6C. The isolate was revealed to be biochemically distinct from previously described mycobacterial species: it has urease and Tween hydrolysis activities and lacks nitrate reductase, 3-day arylsulfatase and b-glucosidase activities.

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Mycobacterium tuberculosisComplex,Mycobacterium leprae, and Other Slow-Growing Mycobacteria

Pfyffer¹,

Vincent²

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Cited by 13 publications

References 24 publications

Characterization of a Novel Group of Mycobacteria and Proposal of Mycobacterium sherrisii sp. nov

Characterization of a Novel Group of Mycobacteria and Proposal of Mycobacterium sherrisii sp. nov

Mycobacterium parmense sp. nov.

Mycobacterium tuberculosisComplex,Mycobacterium leprae, and Other Slow-Growing Mycobacteria

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