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Stouthamer, Richard; Hu, Jin; Kan, F.J.P.M. van; Platner, G. R.; Pinto, John D.

doi:10.1023/a:1009937108715

Cited by 182 publications

(50 citation statements)

References 22 publications

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“…The variation in number of microsatellite repeats seems to be common in ITS sequences, similar microsatellite variation was for instance also found in Eriophyid mites (Fenton et al, 1997). The range obtained here confirms the low intraspecific variation in the ITS2 length detected by Stouthamer et al (1999) and Silva et al (1999). According to Stouthamer et al (1999) ITS2 can be used for species identification in Trichogramma because the sequence variation within species is small relative to the difference found between species.…”

Section: Discussionsupporting

confidence: 78%

“…The range obtained here confirms the low intraspecific variation in the ITS2 length detected by Stouthamer et al (1999) and Silva et al (1999). According to Stouthamer et al (1999) ITS2 can be used for species identification in Trichogramma because the sequence variation within species is small relative to the difference found between species. All morphologically distinct cryptic species are also distinguished by sequence differences, however in the morphologically indistinguishable species North American species T. minutum Riley and T. platneri Nagarkatti no consistent differences were found in their ITS2 sequences (Stouthamer et al, 2000).…”

Section: Discussionsupporting

confidence: 78%

“…The usefulness of endonucleases analysis for distinguishing Trichogramma species was shown by Stouthamer et al (1999), Silva et al (1999) and Pinto et al (2002). How reliable are such molecular keys that are based in some cases on the sequence of only a few lines?…”

Section: Discussionmentioning

confidence: 99%

“…Ribosomal DNA is present in all organisms and is composed of several regions (genes and spacers) that evolve at different rates (Hillis and Dixon, 1991); (5) The internal transcribed spacer regions (ITS-1 and ITS-2) have been used at species and intraspecific levels of many taxa for distinguishing the different forms (Carbone and Kohn, 1993;Bowles and McManus, 1993;Hsiao et al, 1994;Buckler IV et al, 1997). In insects these sequences have been often used for taxonomic purposes (Campbell et al, 1993;Hoy, 1994;Kuperus and Chapco, 1994;Vogler and DeSalle, 1994;Stouthamer et al, 1999;Silva et al, 1999;Chang et al, 2001;Pinto et al, 2002;Honda et al, 2006;Dem'yanchuk et al, 2008;Kumar et al, 2009;Polaszek et al, 2011;Poorjavad et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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ITS-2 sequences-based identification of Trichogramma species in South America

Almeida

Stouthamer²

2015

Braz. J. Biol.

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ITS2 (Internal transcribed spacer 2) sequences have been used in systematic studies and proved to be useful in providing a reliable identification of Trichogramma species. DNAr sequences ranged in size from 379 to 632 bp. In eleven T. pretiosum lines Wolbachia-induced parthenogenesis was found for the first time. These thelytokous lines were collected in Peru (9), Colombia (1) and USA (1). A dichotomous key for species identification was built based on the size of the ITS2 PCR product and restriction analysis using three endonucleases (EcoRI, MseI and MaeI). This molecular technique was successfully used to distinguish among seventeen native/introduced Trichogramma species collected in South America.Keywords: molecular markers, ITS-2 sequence, Hymenoptera, egg parasitoid. Identificação molecular de espécies de Trichogramma na América do Sul via sequenciamento da região ITS-2 ResumoSequências do Espaço Transcrito Interno 2 (ITS2) têm sido utilizadas em estudos taxonômicos e sua utilidade constatada pela confiabilidade que o método confere à identificação das espécies de Trichogramma. Esta técnica molecular foi bem sucedida em distinguir dezessete espécies nativas e introduzidas de Trichogramma, coletadas na América do Sul. As sequências do DNAr variaram de 379 a 632 pb. Em 11 linhagens de T. pretiosum estudadas, o endosinbionte Wolbachia foi detectado pela primeira vez. Estas linhagens telítocas foram encontradas no Peru (9), Colômbia (1) e Estados Unidos (1). Uma chave dicotômica para identificação de espécies foi construída baseada no tamanho do produto da PCR do ITS2 e em análises de restrição utilizando-se três endonucleases (EcoRI, MseI and MaeI).Palavras-chave: marcadores moleculares, sequências ITS-2, Hymenoptera, parasitóide de ovos.

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Section: Discussionsupporting

confidence: 78%

Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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ITS-2 sequences-based identification of Trichogramma species in South America

Almeida

Stouthamer²

2015

Braz. J. Biol.

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“…Los parasitoides obtenidos se depositaron en alcohol al 75 %. La identificación se realizó con base en características morfológicas y claves dicotómicas (Pinto, 1998); ésta se confirmó mediante la secuenciación de la región intergénica ITS2 (Stouthamer, Hu, Van Kan, Platner, & Pinto, 1999). Los datos se analizaron mediante estadísticas descriptivas y el conocimiento biológico de las especies.…”

Section: Methodsunclassified

Conservando la mariposa monarca (Danaus plexippus l.), conservando enemigosnaturales de plagas

Cortéz-Madrigal

García-González

Guzmán-Larralde

2014

Rev Cha Se Cie For y del Amb

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El potencial de la mariposa monarca (Danaus plexippus L.) como hospedera alterna de enemigos naturales de plagas, se investigó (marzo de 2012 a marzo de 2013) en una plantación de Asclepias curassavica L. en la Ciénega de Chapala, Villamar, Michoacán. Para ello, se colectaron muestras de huevos del fitófago y se incubaron en cajas Petri para el registro de parasitismo. Los resultados indican que D. plexippus estuvo presente durante todo el año en la región de estudio, con los niveles máximos de oviposturas durante agosto-diciembre. Trichogramma pretiosum Riley fue el parasitoide predominante, con niveles de parasitismo hasta de 100 %; su mayor actividad coincidió con los niveles máximos de oviposturas del fitófago. La emergencia múltiple de parasitoides en cada huevo de la mariposa contribuye al incremento de las poblaciones de T. pretiosum en campo. Basados en ello, D. plexippus puede considerarse un excelente hospedero alterno de T. pretiosum, enemigo natural de lepidópteros plaga. Adicionalmente, la estrategia propuesta busca contribuir con la conservación e incremento de las poblaciones de la monarca, tanto mediante la conservación de su hospedera A. curassavica como por la eventual reducción en el uso de insecticidas para eliminar plagas.

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Parasitoid Biodiversity and Insect Pest Management

Heraty

2017

Insect Biodiversity

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Untitled

Cited by 182 publications

References 22 publications

ITS-2 sequences-based identification of Trichogramma species in South America

ITS-2 sequences-based identification of Trichogramma species in South America

Conservando la mariposa monarca (Danaus plexippus l.), conservando enemigosnaturales de plagas

Parasitoid Biodiversity and Insect Pest Management

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