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Mroginski, Erika; Rey, H. Y.; Mroginski, L. A.

doi:10.1023/a:1022911230752

Cited by 6 publications

(1 citation statement)

References 12 publications

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“…But few studies have focused on the development of tissue culture methods for T. ciliata. The stems of mature trees or seedlings have been used as explants; however, low regeneration frequencies and difficulty in disinfecting have limited the methodological development (Angeloni et al 1992;Chen et al 2014;Liu et al 2014a;Mroginski et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Development of an efficient regeneration system for the precious and fast-growing timber tree <i>Toona ciliata</i>

Shang

Zhou

et al. 2018

Plant Biotechnology

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Toona ciliata (Chinese mahogany) is an important timber species and secondary protected plant due to excessive exploitation in China. Here we developed a robust and efficient regeneration system for adventitious shoot induction using hypocotyl explants of T. ciliata. To facilitate plant growth, different regulators were added to MurashigeSkoog (MS) medium (0.5 mg/l 6-BA, 1.0 mg/l KT and 0.1 mg/l IBA). A regeneration frequency of 58.67% with four shoots per explant was achieved by horizontal setting of hypocotyls on MS medium and following a 20-day seeding period. MS medium supplemented with 0.3 mg/l 6-BA and 0.2 mg/l NAA was optimal for shoot multiplication and elongation, with a multiplication coefficient of 3.06. A rooting frequency of 93.33% was achieved using the half-strength MS containing 0.1 mg/l NAA. After acclimatization, plantlets were transplanted to sterilized nutrient soil containing a 2 : 1 ratio of vermiculate with 90% survival frequency. Thus, the regeneration system developed in this study would be useful for genetic transformation and other biotechnology endeavours in T. ciliata.

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Section: Introductionmentioning

confidence: 99%