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Romanovskaya, V. A.; Rokitko, P. V.; Mikheev, А. N.; Gushcha, N. I.; Malashenko, Yu. R.; Chernaya, N. A.

doi:10.1023/a:1020575223365

Cited by 29 publications

(11 citation statements)

References 10 publications

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“…The pink-colored representatives of the genus Methylobacterium typically display a high resistance to H 2 O 2 , UV, and ionizing radiation [19]. However, we found statistically significant distinctions (P < 0.05) between the resistance of M. dichloromethanicum DM4, a DCM degrader, and of M. extorquens AM1, incapable of utilizing DCM, to the action of hydrogen peroxide.…”

Section: Resistance Of Methylobacteria To H 2 O 2 and Uv Irradiationmentioning

confidence: 51%

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Effects of DNA-damaging Agents on Aerobic Methylobacteria Capable and Incapable of Utilizing Dichloromethane

Firsova

Torgonskaya

Доронина

et al. 2005

Appl Biochem Microbiol

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Methylobacterium dichloromethanicum DM4, a degrader of dichloromethane (DCM), was more tolerant to the effect of H 2 O 2 and UV irradiation than Methylobacterium extorquens AM1, which does not consume DCM. The addition of CH 2 Cl 2 to methylobacteria with active serine, ribulose monophosphate, and ribulose bisphosphate pathways of C 1 metabolism, grown on methanol, resulted in a 1.1-to 2.5-fold increase in the incorporation of [ α -32 P]dATP into DNA by the Klenow fragment (exo -). Since DCM dehalogenase was not induced in this process, the increase in the total lengths of DNA gaps resulted from the action of DCM rather than S -chloromethylglutathione (intermediate of primary dehalogenation). The degree of DNA damage in the presence of CH 2 Cl 2 was lower in DCM degraders than methylobacteria incapable of degrading this pollutant. This suggests that DCM degraders possess a more efficient mechanism of DNA repair.

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Section: Resistance Of Methylobacteria To H 2 O 2 and Uv Irradiationmentioning

confidence: 51%

“…Although the mechanisms of their injurious effects are different, the DNA-repair systems are to a certain degree analogous. Therefore, the strains resistant to one DNA-damaging factor are frequently tolerant to another physicochemical agent [7][8][9].…”

mentioning

confidence: 99%

Effects of DNA-damaging Agents on Aerobic Methylobacteria Capable and Incapable of Utilizing Dichloromethane

Firsova

Torgonskaya

Доронина

et al. 2005

Appl Biochem Microbiol

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“…The numbers of cultivable Pseudomonas spp. showed a decline in samples from all 3 soils at doses of 80 and 160 Gy, this group has previously been shown as sensitive to γ-radiation [8]. The decline in Pseudomonas spp.…”

Section: Discussionmentioning

confidence: 63%

“…Firstly, variations in γ-penetration through the different soils as a consequence of soil physicochemical characteristics and soil moisture. This would lead to differences in both the direct ionisation of cells and the indirect affects of radiation through formation of free radicals and peroxides [10]. Jackson et al [11] reported that lower radiation doses eliminated microbial populations in wet soils than in dry soils due to the increased formation of free radicals in intra and extracellular fluids.…”

Section: Discussionmentioning

confidence: 99%

“…Previous work on the perturbation of soil microbial communities by ionising radiation has mainly focussed on the impacts of high doses (kGy) designed to sterilise soil for the purpose of transportation between countries and for soil research experiments [4]. A study conducted in the aftermath of the Chernobyl accident indicated that the prokaryotic microbes exhibited reduced species richness in a 10km zone around the reactor [5]. Few studies have attempted to evaluate the effects of either acute or chronic exposure to γ-radiation on indigenous microbial communities at doses which are representative of those encountered in the vicinity of storage repositories and low level fallout scenarios.…”

Section: Introductionmentioning

confidence: 99%

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Detection of irradiation induced changes on the activity and diversity of soil microbial communities: The effect of soil type

et al. 2005

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Abstract.Our aim was to measure the effects of irradiation treatments on soil communities from three different soils. Undisturbed soil cores from three temperate sites (deciduous and coniferous woodland and grassland) were irradiated to give cumulative doses from 0-160 Gy. Cores were incubated at 15 o C and three cores from each treatment sampled after <1, 3 and 8 d. Soil fungi and heterotrophic bacteria were enumerated and the activity and functional diversity of soil microbial communities assessed in terms of their potential to utilise a range of C-sources. Although no significant treatment effects were observed in the numbers of cultivable fungi or fast growing heterotrophic bacteria, the numbers of cultivable Pseudomonas spp. declined in all three soils after irradiation at 80 and 160 Gy. Microbial communities from the coniferous forest soil also showed a dramatic decrease in the metabolic activity and number of substrates utilised by after irradiation at 160 Gy. Gamma irradiation had a greater affect on microbial communities in the two organic forest soils as compared to the mineral grassland soil, this could be related to variations in the physico-chemical shielding properties and in the indigenous communities in terms of radio-resistant species.

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Defenses against keratinolytic bacteria in birds living in radioactively contaminated areas

Ruiz‐Rodríguez

Møller

Mousseau

et al. 2016

Sci Nat

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Microorganisms have shaped the evolution of a variety of defense mechanisms against pathogenic infections. Radioactivity modifies bacterial communities and, therefore, bird hosts breeding in contaminated areas are expected to adapt to the new bacterial environment. We tested this hypothesis in populations of barn swallows (Hirundo rustica) from a gradient of background radiation levels at Chernobyl and uncontaminated controls from Denmark. Investment in defenses against keratinolytic bacteria was measured from feather structure (i.e., susceptibility to degradation) and uropygial secretions. We studied degradability of tail feathers from areas varying in contamination in laboratory experiments using incubation of feathers with a feather-degrading bacterium, Bacillus licheniformis, followed by measurement of the amount of keratin digested. The size of uropygial glands and secretion amounts were quantified, followed by antimicrobial tests against B. licheniformis and quantification of wear of feathers. Feathers of males, but not of females, from highly contaminated areas degraded at a lower rate than those from medium and low contamination areas. However, feathers of both sexes from the Danish populations showed little evidence of degradation. Individual barn swallows from the more contaminated areas of Ukraine produced the largest uropygial secretions with higher antimicrobial activity, although wear of feathers did not differ among males from different populations. In Denmark, swallows produced smaller quantities of uropygial secretion with lower antimicrobial activity, which was similar to swallow populations from uncontaminated areas in Ukraine. Therefore, barn swallows breeding in contaminated areas invested more in all defenses against keratinolytic bacteria than in uncontaminated areas of Ukraine and Denmark, although they had similar levels of feather wear. Strong natural selection exerted by radioactivity may have selected for individuals with higher defense capacity against bacterial infections during the 30 years since the Chernobyl disaster.

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Cited by 29 publications

References 10 publications

Effects of DNA-damaging Agents on Aerobic Methylobacteria Capable and Incapable of Utilizing Dichloromethane

Effects of DNA-damaging Agents on Aerobic Methylobacteria Capable and Incapable of Utilizing Dichloromethane

Detection of irradiation induced changes on the activity and diversity of soil microbial communities: The effect of soil type

Defenses against keratinolytic bacteria in birds living in radioactively contaminated areas

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