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Abstract

Intravenous injection of chloramine derivatives of amino acids and taurine reduced the mortality rate in mice with thrombosis induced by intravenous injection of ADP or collagen-epinephrine mixture. Intravenous injection of N,N-dichlorotaurine caused 50% inhibition of platelet aggregation induced by ADP and measured in the platelet-enriched plasma in vitro. The antithrombotic effect of chloramine derivatives of amino acids and taurine is related to their ability to suppress functional activity of platelets.

Structure and oxidation capacity of amino acid chloramine derivatives and their effects on platelet aggregation

“…The chloramine derivatives of amino acids react with sulfur-containing groups and modify platelet membrane, which results in generalized inhibition of functional activity of platelets (inhibition of its activation irrespective of agonist nature) [1][2][3][4]10]. Inhibition of aggregation of the platelets depends on their structure and physicochemical parameters of N-chloramine acids such as their molecular weight and van der Waals volume.…”

Antiaggregant effects of biogenic chloramines

“…ADP-induced PA in PRP is inhibited by a number of chloramine derivatives of amino acids, the antiaggregant effect of these compounds depends on their structure [2,6]. First, comparison of antiaggregant activity of biochloramines with different location of chloramine group in the molecule showed that the compounds where the chloramine group is located at a distance of 3-5 carbon atoms from the carboxyl group are most effective.…”

“…First, comparison of antiaggregant activity of biochloramines with different location of chloramine group in the molecule showed that the compounds where the chloramine group is located at a distance of 3-5 carbon atoms from the carboxyl group are most effective. The existence of a negatively charged group at the site of biochloramine interaction with the plasma membrane responsible for this structural feature was hypothesized [6]. Second, it was found that antiaggregant effect of chloramine derivatives of amino acids on platelets in PRP after addition of ADP depends on their molecular weight (size of molecule) [2] (Fig.…”

“…We propose biogenic chloramines as new covalent inhibitors of platelets [2][3][4][5][6][7][8][9][10][11].…”

“…At the same time, there are data on modifying effects of chloramines on some cells [17,23]. We showed that chloramine derivatives of amino acids inhibit aggregation capacity of rabbit and human platelets [2,3,5,6,[9][10][11].Here we compared the effects of chloramine derivatives of amino acids and taurine on platelets, erythrocytes, and leukocytes, studied the mechanism of the antiaggregant effect of chloramine derivatives on platelets, and initial selectivity of the antiaggregant effect of biogenic chloramines. …”