Untitled

Abstract

Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Esmolol hydrochloride (ESM), Ramipril (RAM), Gemifloxacin (GEM), Terazosin (TRZ) and Moxifloxacin (MOX), based on their reactivity towards KMnO 4 . Beer's law is obeyed in the concentration of 4-28, 4-28, 2.6-18.2, 1.5-10.5 and 1-7 μg ml-1 for Esmolol hydrochloride, Ramipril, Gemifloxacin, Terazosin and Moxifloxacin. The methods involve the addition of excess KMnO 4 of known concentration in the presence of 2M H2SO 4 , reactants are allowed to react and the unreacted KMnO 4 is estimated with a fixed amount of Methyl Orange by measuring the absorbance at 507nm. Results of analyses were validated statistically. Statistical comparison of the results with the reference method shows excellent agreement and indicates no significant difference in accuracy and precision. These methods have been applied for the determination of drugs in their pure form as well as in tablet formulations. These methods have been validated in terms of guidelines of ICH.