Untitled

Abstract

The present study was to evaluate the in vitro cholesterol esterase enzyme inhibitory and in vitro antioxidant activity of the methanol extract of the leaves of Camellia sinensis (L.). Phytochemical screening of the extract shows the presence of flavonoids, phenolics and terpenoids. The extract shows ability to inhibit the enzyme with IC 50 (82.46±0.74µg/ml) where as that of standard, Orlistat (24.15±0.59µg/ml). Antioxidant and free radical scavenging activity were also assessed by using the methods, IC 50 values for Nitric oxide radical scavenging activity (396.83±0.83µg/ml), whereas for standard curcumin (260.38±0.66µg/ml), hydroxyl radical scavenging activity (47.04±2.26µg/ml) and for quercetin (70.99±1.31µg/ml). Moreover, the extract was found to scavenge the superoxide with 50% inhibition at 308.17±23.25µg/ml and standard ascorbic acid at 225.08±2.44µg/ml, IC 50 for ferrous chelating ability assay (44.12±4.63µg/ml) and of ascorbic acid (47.25±.89µg/ml). Total content of flavonoids present in 1mg of extract was 19.8±0.11 µg quercetin equivalents/mg. Results indicated that the extract shows potential bioactive compounds which might have a beneficial impact on diseases related to cholesterol synthesis and showed potential antioxidant and free radical scavenging activities. INTRODUCTION: Dietary cholesterol comprised of free and esterified cholesterol. In diets rich in meats, significant percent of cholesterol is esterified. Hydrolysis of cholesteryl ester in the lumen is catalyzed by cholesterol esterase (CEase).Cholesterol esterase (CEase) also is a lipolytic enzyme with wide substrate specificity capable of hydrolysing cholesteryl esters, triacylglycerol, phospholipids and lysophospholipids. It is synthesized primarily by the pancreas and is found as a component of pancreatic juice.This enzyme is also found in the milk of numerous species. Milk CEase is postulated to substitute for the pancreatic enzyme in the neonatal gastrointestinal tract before maturation of the pancreas 1 . Pancreatic cholesterol esterase is also known as bile salt-activated lipase, nonspecific lipase, phospholipase A 1 lysophospholipase, bile salt-dependent lipase, bile salt stimulated lipase, carboxyl ester lipase, and carboxyl ester hydrolase 2 .A number of studies have suggested a possible role for CEase in the absorption of free cholesterol at the brush border membrane of the small intestine 3 . Cholesterol and its esters are the major risk factors for the development of various cardiovascular diseases and are known to be one of the leading causes of death in developing and developed countries. Free radical is an atom with at least one unpaired electron in the outer

Anti-inflammatory, cytotoxicity and antilipidemic properties: novel bioactivities of true cinnamon (Cinnamomum zeylanicum Blume) leaf

“…Therefore, this is novel finding may help in managing obesity and hyperlipidemia in the world. Dietary fat absorption in the small intestine is a multi-step process and can be regulated through inhibition of pancreatic cholesterol esterase [ 57 ], cholesterol micellization [ 58 , 59 ] and bile acid binding [ 35 ]. Investigated both leaf extracts of true cinnamon had moderate cholesterol esterase and cholesterol micellization inhibitory activities and ethanolic leaf extract showed the highest activity.…”