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Abstract

Background/Aim: Endosulfan is a pesticide of organochlorine group uses by 55 % of farmers in Bihar. Present study aims to illustrate effect of Endosulfan on biochemical, hormonal and sub cellular anomalies of spermatozoa of mice and their restoration through root extract of Withania somnifera. Materials and Methods: Experimental mice were administered with endosulfan for eight weeks followed by eight weeks administration of Withania somnifera. Results: Endosulfan administered group show degenerated mitochondria and plasmamembrane. Degenerated microtubule were also observed with rudimentary central hub and dynein arm. While eight weeks Withania somnifera 1000 mg/kg/b.w/day administered group show greater degree of sub cellular restoration on mitochondria and nuclear membrane. Microtubules were almost normal in structure. Lipid peroxidation level were also restored toward normal after ashwagandha administration. Conclusion: These combined effect finally leading to restoration in structure of spermatozoa in mice. This is very effective in restoring male fertility by combating endosulfan toxicity.