Through the magnetic measurements, isochronal annealing experiments in the temperature 25950 ºC of deformed, quenched, pre-annealed and pulsed laser damage samples Fe50Ni50 alloy revealed the existence of three annealing stages I, II and III in the annealing spectrum of heavily cold-worked by observing the associated changes in maximum magnetic permeability (µmax) on the magnetic field(H) for different annealing temperature. The first annealing stage I appeared in the temperature range from125-500 ºC and it was activated by energy 1.05 eV. It was attributed to the short range order caused by the long range migration of vacancies. The second annealing stage II appeared in the temperature range from 550-750 ºC and it was activated by 1.82 eV, it is associated with an increase in µmax due to the dissociation of vacancy clusters formed during stage I. The third annealing stage III appeared in the temperature range from 750-825 ºC and it was activated by 3.04 eV, it was related to the climb motion of dislocation during the recrystallization process.