Vaccination against <i>Fasciola hepatica</i> infection using a <i>Schistosoma mansoni</i> defined recombinant antigen, Sm14

Almeida, Marília Sirianni dos Santos; Torloni, Humberto; Lee‐Ho, Paulo; Vilar, Mônica Magno; Thaumaturgo, Nilton; Simpson, Andrew J.G.; Tendler, Míriam

doi:10.1046/j.1365-3024.2003.00619.x

Cited by 45 publications

(28 citation statements)

References 12 publications

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“…The presence of high levels of antibodies to F. hepatica ES antigens in the animals that acquired immunity against infection suggests that antibodies could have contributed to the protection. This is consistent with observations made by other authors who, using immunogens other than FhSAP2, found associations between protection and the levels of antibodies (Acosta et al, 2008; Almeida et al, 2003; Law et al, 2003; Martinez-Fernandez et al, 2004; Meeusen and Piedrafita, 2003; Ramajo et al, 2001; Reszka et al, 2005; Sexton et al, 1994; Smooker et al, 2004; Vilar et al, 2003; Zafra et al, 2008). Because FhSAP2 is a lytic protein, FhSAP2 could be involved in acquiring nutrition for the liver fluke and/or its migration through the liver parenchyma (Espino and Hillyer, 2003).…”

Section: Discussionsupporting

confidence: 93%

Quantitation of cytokine mRNA by real-time RT-PCR during a vaccination trial in a rabbit model of fascioliasis

Espino¹,

Rivera²

2010

Veterinary Parasitology

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Use of the rabbit as disease model has long been hampered by a lack of immunological assays specific to this species. In the present study we developed a SYBR Green-based, real-time RT-PCR protocol to quantitate cytokine mRNA in freshly harvested rabbit peripheral mononuclear cells. The method was validated in the course of a vaccination trial in which animals vaccinated with the recombinant antigen FhSAP2 were challenged with Fasciola hepatica metacercariae. Changes in the levels of rabbit interleukin (IL)-2, IL-4, IL-6, IL-10, tumor necrosis factor-alpha (TNFα), and interferon-gamma (IFNγ) mRNA were determined. Messenger RNA from the universally expressed housekeeping gene GAPDH was used as an amplification control and allowed for correction of variations in the efficiencies of RNA extraction and reverse transcription. Rabbits vaccinated with FhSAP2 showed an 83.3% reduction in liver fluke burden after challenge infection when compared to non-vaccinated controls. All cytokine mRNAs were found at detectable levels; however, the levels of IFNγ, TNFα, IL-2 and IL-10 were significantly higher in the vaccinated group compared to the non-vaccinated group. These results suggest that protection conferred by FhSAP2 protein could be associated with a mixed Th1/Th2 immune response in which Th1 cytokines are dominant. The real-time RT-PCR method described herein can be a useful tool for monitoring changes in basic immune functions in the rabbit model of fascioliasis and may also aid in studies of human diseases for which the rabbit is an important experimental model.

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Section: Discussionsupporting

confidence: 93%

Quantitation of cytokine mRNA by real-time RT-PCR during a vaccination trial in a rabbit model of fascioliasis

Espino¹,

Rivera²

2010

Veterinary Parasitology

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“…These include Sm23 [39-45], integral membrane protein 25 [46], Sm14 [47,48], and 26 and 28 glutathione S-transferases [49-52]. …”

Section: Resultsmentioning

confidence: 99%

Gene expression patterns during adaptation of a helminth parasite to different environmental niches

et al. 2007

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“…However, these strong cellular and humoral immune responses did not protect against future challenges. Recently, high levels of protection have been obtained in sheep with purified cathepsin L proteases [8,10,17,25] and recombinant antigen Sm14 [1]. Nonetheless, their specific protective mechanisms are yet to be elucidated.…”

Section: Discussionmentioning

confidence: 99%

“…These studies reported that sheep do not develop resistance to reinfection. However, more recent studies have demonstrated that some immunisation protocols induce a significant protective response against challenge infection in sheep [1,25] and cattle [19,20]. Therefore, there are many questions concerning the immunological basis of susceptibility and resistance in sheep that should be elucidated [1,8,9,18].…”

Section: Introductionmentioning

confidence: 99%

Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

Pérez¹,

Ortega²,

Bravo³

et al. 2005

Vet. Res.

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-The phenotype of the hepatic inflammatory infiltrate and hepatic lymph nodes (HLN) was analysed in lambs primarily and challenge infected with Fasciola hepatica. Group 1 was primarily and challenge infected with two doses of 200 metacercariae (mc) each and was nontreated. Trickle infection was administered to five groups: group 2 was challenge infected and nontreated; group 3 was primarily infected and non-treated; group 4 was primarily infected and treated with triclabendazole (TCBZ) at 12 weeks postinfection (wpi); group 5 was treated at 4 wpi and challenge infected and group 6 was treated at 12 wpi and challenge infected. An uninfected group was used as the control. The distribution of T cell subpopulations (CD3, CD4 and CD8), and B cells (CD79α, IgM, IgG) was analysed. The hepatic inflammatory infiltrate was represented mainly by CD3 and CD4 T cells, and B cells (CD79α, IgG). These infiltrates were more severe (P < 0.05) in primarily (group 3) or challenge (groups 2, 5 and 6) trickle infected lambs than in the group single challenge infected (group 1). Cellular changes in HLN consisted in an increase of CD4 over CD8 T cells and an increase of B cells and IgG + plasma cells, and they were more severe in primarily and challenge trickle infected groups than in the group infected with two larger doses of mc, although significant differences were not found with respect to all challenge trickle infected groups. The strong local cellular and humoral immune responses did not protect against subsequent infections, neither in non-treated lambs (group 2) nor in lambs treated with TCBZ at 4 wpi (group 5) or 12 wpi (group 6).

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Vaccination against Fasciola hepatica infection using a Schistosoma mansoni defined recombinant antigen, Sm14

Cited by 45 publications

References 12 publications

Quantitation of cytokine mRNA by real-time RT-PCR during a vaccination trial in a rabbit model of fascioliasis

Quantitation of cytokine mRNA by real-time RT-PCR during a vaccination trial in a rabbit model of fascioliasis

Gene expression patterns during adaptation of a helminth parasite to different environmental niches

Phenotype of hepatic infiltrates and hepatic lymph nodes of lambs primarily and challenge infected with Fasciola hepatica, with and without triclabendazole treatment

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