Vaccination confers significant protection of sheep against infection with a virulent United Kingdom strain of Corynebacterium pseudotuberculosis

Fontaine, Michaël C.; Baird, Graham; Connor, Kathleen M.; Rudge, Karen; Sales, Jill; Donachie, W.

doi:10.1016/j.vaccine.2006.05.005

Cited by 50 publications

(52 citation statements)

References 25 publications

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“…In the present study, from the 202 samples collected, 35 were from mediastinal lymph nodes, and from these 28 (80%) were positive for C. pseudotuberculosis. These data corroborate the findings of Fontaine et al (2006), who described that experimentally infected animals with C. pseudotuberculosis by subcutaneous route developed primary foci infection in mediastinal lymph nodes, followed by the lungs. Stoops et al (1984) also reported that only 26% of the animals with caseous lymphadenitis had apparent symptoms.…”

Section: Discussionsupporting

confidence: 92%

Diagnostic comparison of Corynebacterium pseudotuberculosis through microbiological culture and PCR in sheep samples

et al. 2016

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Caseous lymphadenitis is a chronic infectious disease caused by Corynebacterium pseudotuberculosis , which is a bacterium responsible for a great number of economic losses on goat and sheep production. It is characterized by the formation of abscesses in superficial lymph nodes and in internal organs and lymph nodes. This study aimed at determining the agreement between microbiological culture and PCR in the identification of C. pseudotuberculosis , in samples collected from animals in slaughterhouses and in animals that presented lymph node enlargement in field conditions. From the 202 samples analyzed through microbiological culture, 113 (56%) were positive for Corynebacterium sp.; from these positive samples, 38 (34%) were identified as C. pseudotuberculosis by microbiological culture. From the amount of samples, 110 (54%) were positive and 92 (46%) were negative in the PCR. Kappa index (0.193) presented a weak agreement between PCR and microbiological culture. We concluded that molecular diagnosis (PCR) in clinical samples proved to be more efficient, reproducible, and faster than the microbiological culture, both on clinical samples analyses and in the confirmation of C. pseudotuberculosis in colonies that were classified by Corynebacterium genus. Thus, the present study demonstrated the importance of PCR to confirm C. pseudotuberculosis diagnosis, and the best contribution for the epidemiological surveillance of the disease in sheep.

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Section: Discussionsupporting

confidence: 92%

Diagnostic comparison of Corynebacterium pseudotuberculosis through microbiological culture and PCR in sheep samples

et al. 2016

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“…This protocol differs from those previously used to produce rPLD (25)(26)(27). Soluble rPLD was expressed using this protocol, in contrast with some other studies (25,27) but similar to the results reported by Songer et al (26). However, Songer et al found that the enzyme activity of the C. pseudotuberculosis culture supernatant was approximately 2.5-fold more effective than that of E. coli (pCpO5O).…”

Section: Discussionsupporting

confidence: 73%

Comparison of the efficiency of concentrated soluble recombinant phospholipase D and natural phospholipase D enzymes

Sakmanoğlu¹,

Erganiş²

2016

Turk J Vet Anim Sci

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“…a phospholipase (BP0878) and BipA. Although BP0878 may also have vaccine potential, 48 we only assessed the vaccine potential of BipA because it was more abundantly expressed under biofilm conditions. Further, BipA was also found upregulated in Tohama I biofilms relative to planktonic cultures suggesting that expression of BipA is a common signature of Bordetella biofilms.…”

Section: Discussionmentioning

confidence: 99%

The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins

Gouw

Serra

Jonge

et al. 2014

Emerging Microbes & Infections

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Pertussis is an infectious respiratory disease of humans caused by the gram-negative pathogen Bordetella pertussis. The use of acellular pertussis vaccines (aPs) which induce immunity of relative short duration and the emergence of vaccine-adapted strains are thought to have contributed to the recent resurgence of pertussis in industrialized countries despite high vaccination coverage. Current pertussis vaccines consist of antigens derived from planktonic bacterial cultures. However, recent studies have shown that biofilm formation represents an important aspect of B. pertussis infection, and antigens expressed during this stage may therefore be potential targets for vaccination. Here we provide evidence that vaccination of mice with B. pertussis biofilm-derived membrane proteins protects against infection. Subsequent proteomic analysis of the protein content of biofilm and planktonic cultures yielded 11 proteins which were ≥three-fold more abundant in biofilms, of which Bordetella intermediate protein A (BipA) was the most abundant, surface-exposed protein. As proof of concept, mice were vaccinated with recombinantly produced BipA. Immunization significantly reduced colonization of the lungs and antibodies to BipA were found to efficiently opsonize bacteria. Finally, we confirmed that bipA is expressed during respiratory tract infection of mice, and that anti-BipA antibodies are present in the serum of convalescent whooping cough patients. Together, these data suggest that biofilm proteins and in particular BipA may be of interest for inclusion into future pertussis vaccines.

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Vaccination confers significant protection of sheep against infection with a virulent United Kingdom strain of Corynebacterium pseudotuberculosis

Cited by 50 publications

References 25 publications

Diagnostic comparison of Corynebacterium pseudotuberculosis through microbiological culture and PCR in sheep samples

Diagnostic comparison of Corynebacterium pseudotuberculosis through microbiological culture and PCR in sheep samples

Comparison of the efficiency of concentrated soluble recombinant phospholipase D and natural phospholipase D enzymes

The vaccine potential of Bordetella pertussis biofilm-derived membrane proteins

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