Vaccination‐induced HI antibody response to intraepidemic influenza A(H3N2) virus variants of the 1996–1997 epidemic season

Abstract

Intraepidemic antigenic and genetic variation was indicated when H3N2-subtype influenza A virus strains isolated during the 1996-1997 epidemic season in Finland were studied for reactivity in the haemagglutination inhibition (HI) assay and for nucleotide sequences coding for the variable HA1 domain of viral haemagglutinin. Thirty prevaccination- and postvaccination-paired sera taken from subjects who had been vaccinated against influenza during the previous autumn were studied for the presence of HI antibody to the homologous vaccine virus A/Nanchang/933/95, and five field strains representing the genetic and antigenic variability of the 1996-1997 epidemic season. The lowest vaccination-induced HI titres in each of the three age groups were detected in the two field strains that had been isolated from vaccinated patients and belonged to two different genetic sublineages. The intraepidemic variability of the 1996-1997 field strains in HI reactivity may be indicative of circulation of virus strains that may be capable of breaking through vaccination-induced immunity better than the other strains.

Influenza vaccination of dialysis patients: cross-reactivity of induced haemagglutination-inhibiting antibodies to H3N2 subtype antigenic variants is comparable with the response of naturally infected young healthy adults

“…The drift variants were isolated and cultivated exclusively in MDCK cell cultures and the vaccine strain in embryonated eggs. Antigenic relationships of the vaccine virus (JHNu33u94) and epidemic viruses were analysed in the HI tests using rat antisera as described previously [11].…”

Vaccine Analysis: Strategies, Principles, and Control

“…It is now well recognised that about 1 % of children have congenital Human herpesvirus 6 (HHV-6) infections and 86 % of these are the result of germline transmission of chromosomally integrated virus (Hall et al 2004;Leong et al 2007;Hall et al 2008). genetically transmitted viruses, require special consideration.…”