Vaccination of Chickens against Ornithobacterium rhinotracheale Infection

Empel, Paul van; Bosch, Han van den

doi:10.2307/1592684

Cited by 33 publications

(20 citation statements)

References 7 publications

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“…rhinotracheale infection with homologous serotypes (34). Because 18 different O. rhinotracheale serotypes have currently been identified (4,33), an immunization strategy is needed that protects birds, especially turkeys, from infections against multiple serotypes.…”

mentioning

confidence: 99%

Successful Selection of Cross-Protective Vaccine Candidates for Ornithobacterium rhinotracheale Infection

Schuijffel¹,

Empel²,

Pennings³

et al. 2005

Infect Immun

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Ornithobacterium rhinotracheale is a bacterial pathogen known for causing respiratory disease in poultry. In this study, we demonstrate for the first time that cross-protective immunity against different O. rhinotracheale serotypes can be induced by live vaccination. Sera from these live-vaccinated and cross-protected birds were used to identify new vaccine targets by screening an O. rhinotracheale expression library. Out of 20,000 screened plaques, a total of 30 cross-reactive clones were selected for further analysis. Western blot analysis and DNA sequencing identified eight different open reading frames. The genes encoding the eight cross-reactive antigens were amplified, cloned in an expression vector, and expressed in Escherichia coli. Purified recombinant proteins with a molecular mass ranging from 35.9 kDa to 62.9 kDa were mixed and tested as a subunit vaccine for (cross-)protection against challenge with homologous and heterologous O. rhinotracheale serotypes in chickens. Subunit vaccination resulted in the production of antibodies reactive to the recombinant proteins on Western blot, and this eight-valent vaccine conferred both homologous and heterologous protection against O. rhinotracheale challenge in chickens.

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mentioning

confidence: 99%

Successful Selection of Cross-Protective Vaccine Candidates for Ornithobacterium rhinotracheale Infection

Schuijffel¹,

Empel²,

Pennings³

et al. 2005

Infect Immun

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“…causing enough protection in the progeny against experimental challenges (van Empel and van den Bosch, 1998;Cauwerts et al, 2002;Bisshop, 2003). In addition, Cauwerts et al (2002) found lower condemnation rates, lower mean mortality and higher mean production index in the progeny from vaccinated broiler breeders.…”

Section: Vaccinationmentioning

confidence: 91%

“…However, in spite of the availability of autogenous vaccines, economic losses related to O. rhinotracheale infections in the poultry industry are estimated in hundreds of millions of dollars annually in the United States (Tabatabai et al, 2010). Bacterins, live vaccines, and subunit recombinant vaccines have been developed and reported, with variable results for the control of experimental and natural infections associated with O. rhinotracheale (van Empel and van den Bosch, 1998;Cauwerts et al, 2002;Lopes et al, 2002;Bisshop, 2003;Chansiripornchai, 2004;Schuijffel et al, 2006a;2006b;Murthy et al, 2007).…”

Section: Vaccinationmentioning

confidence: 99%

<i>Ornithobacterium rhinotracheale</i> Infection in Poultry: an Updated Review

Churria¹,

Machuca²,

Vigo³

et al. 2012

ijmz

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Ornithobacterium rhinotracheale is a Gram-negative bacterium of the rRNA superfamily V within the CytophagaFlavobacterium-Bacteroides phylum, which has become an emerging pathogen in the poultry industry. The purpose of this work is to review the current literature on O. rhinotracheale infection in domestic poultry. Despite the difficulties to identify the bacteria using biochemical tests, the polymerase chain reaction (PCR) technique has been successfully used to identify suspected isolates. Moreover, pulsed-field gel electrophoresis (PFGE) has allowed the genetic typing of O. rhinotracheale. Recent investigations carried out in the United States and Argentina described the unusual and extensive β-hemolytic activity of field strains isolated from affected chickens and turkeys. According to the latest serological studies, the causing agent has eighteen serotypes (A to R) and serotype A is the most prevalent among chicken and turkey strains. Gross findings typically found in broiler chickens are unilateral pneumonia, pleuritis, and abdominal airsacculitis with foamy, white yogurt-like exudate, which cause increased condemnation rates at slaughter, whereas those found in turkeys include tracheitis, pneumonia or bronchopneumonia, thoracic and/or abdominal airsacculitis, pericarditis, and peritonitis. Despite the worldwide efforts to control O. rhinotracheale infection, the present situation shows several problems not only because antibiotic treatments appear to be less effective due to the resistance acquired in the poultry industry but also because the vaccines developed so far show variable results in commercial poultry.

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“…Previous work done under controlled conditions in the Netherlands indicated that breeder hens were able to transfer a significant and protective immunity to their broiler progeny after vaccination with the bacterin. 11 The objective of this study was to confirm the safety (through clinical monitoring of vaccinated breeders) and efficacy (serologically and by evaluating the effect on broiler production) of the ORT bacterin under large-scale commercial conditions in South Africa.…”

Section: Introductionmentioning

confidence: 99%

The use of a bacterin vaccine in broiler breeders for the control of Ornithobacterium rhinotracheale in commercial broilers

Vuuren¹,

Gummow²

2004

J. S. Afr. Vet. Assoc.

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<em>Ornithobacterium rhinotracheale</em> (ORT) is a recently identified bacterial pathogen of poultry, linked to the respiratory disease complex of broilers and the economic losses associated with that disease complex. Present control measures applied for the disease include the continuous use of in-feed antibiotics. A recently developed bacterin vaccine that is applied to broiler-breeder hens to pass on protective immunity to their broiler progeny was tested under large-scale commercial conditions in South Africa. An indirect ELISA test for antibodies to ORT, optimised for use in South Africa, was used to determine antibody levels in breeders and broilers. ELISA test results showed that the vaccine stimulated the development of high antibody titre levels in broiler breeders. The efficacy of the vaccine in protecting the progeny of these birds from ORT challenge could not be determined during the trial, although the progeny of vaccinated hens appeared to perform slightly better under commercial conditions than the progeny of unvaccinated hens

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Vaccination of Chickens against Ornithobacterium rhinotracheale Infection

Cited by 33 publications

References 7 publications

Successful Selection of Cross-Protective Vaccine Candidates for Ornithobacterium rhinotracheale Infection

Successful Selection of Cross-Protective Vaccine Candidates for Ornithobacterium rhinotracheale Infection

<i>Ornithobacterium rhinotracheale</i> Infection in Poultry: an Updated Review

The use of a bacterin vaccine in broiler breeders for the control of Ornithobacterium rhinotracheale in commercial broilers

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