Vaccination of dogs against Babesia canis infection

Schetters, Theo; Kleuskens, J.; Scholtes, N.; Pasman, J.W.; Goovaerts, D.

doi:10.1016/s0304-4017(97)00044-7

Cited by 33 publications

(28 citation statements)

References 14 publications

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“…Initially, a drop in haematocrit and swellings of the lymph nodes and spleen were observed in the dogs that had been vaccinated and then infected with virulent protozoa. As antibodies specific to SPA appeared in the serum of the studied animals, the number of erythrocytes increased, the swellings of lymph nodes and spleen receded, and the blood flow through blood vessels improved (18). These results clearly demonstrate that the SPA antibodies, formed as a consequence of antigen stimulation, prevent the development or exacerbation of the pathological processes presented above.…”

Section: Discussionsupporting

confidence: 54%

“…This antigen was first used to prevent piroplasmosis by Sibinovic et al (25). Studies on its application in the prevention of canine babesiosis have been carried out for years (18,22,23). So far, the way of inducing immunity against babesiosis by this antigen has not been studied in dogs in details.…”

Section: Discussionmentioning

confidence: 99%

“…Studies on the use of soluble parasite antigens (SPA) from plasma of Babesia-infected animals or derived from in vitro cultures of Babesia organisms in immunoprophylaxis of babesiosis have been in progress for quite some time (5,15,16,(22)(23)(24). Early observations showed that vaccinations with SPA protected dogs against the development of a critical pathological process characterised by a decrease in packed cell volumes and retention of infected erythrocytes in the microvasculature (18).…”

mentioning

confidence: 99%

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Quantitative Changes in Selected Lymphocyte Subpopulations after Administration of a Soluble Parasitic Antigen of Babesia Canis to Dogs

Adaszek

Lisiecka²,

Carbonero

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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Changes in selected blood lymphocyte subpopulations in dogs administered with a soluble parasitic antigen (SPA) derived from a supernatant of 18S RNA-A and 18S RNA-B Babesia canis cell culture were investigated. The studies included 20 dogs divided into three groups: group I (n=8) -comprised of dogs receiving SPA twice, at 3 week intervals; group II (n=5) -nonvaccinated control dogs, and group III (n=7) -dogs vaccinated twice with a commercial B. canis vaccine. Cytometric analysis revealed that vaccination with SPA derived from B. canis culture had similar effects to the vaccination with a commercial vaccine. The vaccination lowered the percentage of T lymphocytes (CD3+), T helper cells (CD4+), cytotoxic/supressor T cells (CD8+), B lymphocytes (CD21+), and MHC II lymphocytes in the blood in comparison to non-vaccinated dogs. Statistical analysis of the results demonstrated that mean values of the tested parameters at each stage of the study were similar in groups I and III and significantly higher in group II. The lowered level of the lymphocyte subpopulations in groups I and III persisted during the whole period of the study. The results presented that SPA has immunosuppressive effect in the first period after being administrated.

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Section: Discussionsupporting

confidence: 54%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Quantitative Changes in Selected Lymphocyte Subpopulations after Administration of a Soluble Parasitic Antigen of Babesia Canis to Dogs

Adaszek

Lisiecka²,

Carbonero

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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“…Anaemia in babesiosis has been investigated in numerous studies. Several mechanisms that can cause anaemia have been proposed, such as sequestration in the spleen, autoimmune haemolysis, reduced red cell deformability, haemodilution and increased oxidative damage (Maegraith et al, 1957;Murase et al, 1995;Schetters et al, 1997aSchetters et al, , 1998Schetters et al, , 2009Reyers et al, 1998;Dondrop et al, 1999). Haemodilution as a cause of early anaemia was suggested by Schetters et al (2009).…”

Section: Discussionmentioning

confidence: 99%

“…In experimental B. rossi infection, there was a marked decrease in haematocrit long before parasites were detectible in peripheral blood (Shetters et al, 1997a). This early change was hypothesized to be caused by hemodilution, splenomegaly and sequestration in the spleen (Maegraith et al, 1957;Schetters et al, 1997aSchetters et al, , 1998Schetters et al, , 2009). This mechanism may include autoimmune haemolysis (Reyers et al, 1998), reduced red cell deformability (Dondrop et al, 1999) and increased oxidative damage (Murase et al, 1995;Morita et al, 1996;Otsuka et al, 2001;Rezaei and Dalir-Naghadeh, 2006).…”

mentioning

confidence: 99%

Malondialdehyde levels in serum of dogs infected with Babesia canis

Crnogaj¹,

Petlevski²,

Mrljak³

et al. 2010

Vet. Med.

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Babesiosis is a common tick born disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The aim of the present study was to confirm the presence of oxidative stress by examining serum malondialdehyde (MDA), an end product of lipid peroxidation, in 35 dogs naturally infected with Babesia canis (B. canis). MDA was examined in 14 healthy dogs as well. Blood smears were prepared from peripheral blood and they showed the presence of B. canis in infected dogs. B. canis was confirmed using the PCR (Polymerase chain reaction) method. On the basis of clinical and laboratory data the 35 infected dogs were clinically classified into two groups, complicated (seven dogs) and uncomplicated (28 dogs). The noted complications were renal dysfunction (5/7), hepatic dysfunction (3/7), muscular involvement (2/7) and ARDS (1/7). Levels of blood urea nitrogen concentration (BUN), creatinin, total bilirubin, alanin aminotranspherase (ALT), alkaline phosphatase (AP), and gammaglutamil aminotranspherase (GGT) were significantly increased in dogs with complicated versus uncomplicated babesiosis. Furthermore the uncomplicated group of dogs was, depending on the severity of anaemia, classified as suffering from severe, moderate or mild disease. Levels of serum MDA were significantly higher in sick dogs (36.90 μmol/l ± 13.95) than healthy animals (8.13 μmol/l ± 1.78). There was no significant statistical difference in serum MDA levels between dogs with complicated (38.48 μmol/l ± 12.11) and uncomplicated babesiosis (36.50 μmol/l ± 14.55). Comparison of the groups based on the severity of anaemia showed that there was no significant statistical difference in serum MDA levels between them. The study demonstrated the involvement of oxidative damage in dogs naturally infected with B. canis.

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Canine babesiosis in Romania due to Babesia canis and Babesia vogeli: a molecular approach

et al. 2011

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Canine babesiosis is a tick-borne disease caused by the protozoa Babesia spp. that affects dogs worldwide. In Romania, canine babesiosis has become quite frequent in the last few years, with a wide variety of clinical signs, ranging from mild, nonspecific illness to peracute collapse, and even death. Traditionally, a Babesia infection in dogs is diagnosed based on the morphologic appearance of the intraerythrocytic piroplasms observed in peripheral blood smears. To date, no data on genetic characterization of Babesia species in dogs has been documented for Romania. Therefore, a molecular survey on natural Babesia infections of dogs in Romania using polymerase chain reaction and genetic sequence analysis of a fragment of the ssRNA gene was performed. A total number of 16 blood samples were tested for the presence of Babesia DNA. Blood samples were collected from 11 dogs with symptoms of babesiosis and microscopically proven positive for Babesia and from a group of five asymptomatic dogs, not tested microscopically for Babesia, which were included in the study for comparative analysis. The piroplasm-specific PCR amplifying the partial 18S rRNA gene confirmed Babesia spp. infection in all 11 samples from dogs with clinical babesiosis, and in one of the clinically normal dogs. Sequence analysis revealed the presence of Babesia canis in all clinically affected dogs and Babesia vogeli in one clinically normal dog. This is the first molecular evidence of B. canis and B. vogeli in dogs from Romania. The results of the study provide basic information toward a better understanding of the epidemiology of canine babesiosis in Romania and will help to promote an effective control program.

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Vaccination of dogs against Babesia canis infection

Cited by 33 publications

References 14 publications

Quantitative Changes in Selected Lymphocyte Subpopulations after Administration of a Soluble Parasitic Antigen of Babesia Canis to Dogs

Quantitative Changes in Selected Lymphocyte Subpopulations after Administration of a Soluble Parasitic Antigen of Babesia Canis to Dogs

Malondialdehyde levels in serum of dogs infected with Babesia canis

Canine babesiosis in Romania due to Babesia canis and Babesia vogeli: a molecular approach

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