Vaccination of goats with a combinationSalmonellavector expressing fourBrucellaantigens (BLS, PrpA, Omp19, and SOD) confers protection againstBrucella abortusinfection

Abstract

Salmonella is an intracellular pathogen with a cellular infection mechanism similar to that of Brucella, making it a suitable choice for use in an anti-Brucella immune boost system. This study explores the efficacy of a Salmonella Typhimurium delivery-based combination vaccine for four heterologous Brucella antigens (Brucella lumazine synthase, proline racemase subunit A, outer-membrane protein 19, and Cu/Zn superoxide dismutase) targeting brucellosis in goats. We inoculated the attenuated Salmonella delivery-based vaccine combination subcutaneously at two different inoculation levels; 5 × 109 colony-forming unit (CFU)/mL (Group B) and 5 × 1010 CFU/mL (Group C) and challenged the inoculations with virulent Brucella abortus at 6 weeks post-immunization. Serum immunoglobulin G titers against individual antigens in Salmonella immunized goats (Group C) were significantly higher than those of the non-immunized goats (Group A) at 3 and 6 weeks after vaccination. Upon antigenic stimulation, interferon-γ from peripheral blood mononuclear cells was significantly elevated in Groups B and C compared to that in Group A. The immunized goats had a significantly higher level of protection as demonstrated by the low bacterial loads in most tissues from the goats challenged with B. abortus. Relative real-time polymerase chain reaction results revealed that the expression of Brucella antigens was lower in spleen, kidney, and lung of immunized goats than of non-immunized animals. Also, treatment with our combination vaccine ameliorated histopathological lesions induced by the Brucella infection. Overall, the Salmonella Typhimurium delivery-based combination vaccine was effective in delivering immunogenic Brucella proteins, making it potentially useful in protecting livestock from brucellosis.

Protective efficacy of attenuated Salmonella Typhimurium strain expressing BLS, Omp19, PrpA, or SOD of Brucella abortus in goats

“…To come up with a robust protective immune response that can be close to the long-lived natural infection, the ideal mixture of a few proteins of the pathogen is a difficult and complex undertaking [ 27 ]. In our recent study, we reported that immunization of goat with a combination Salmonella vector strain delivering a concoction of BLS, PrpA, Omp19, and SOD can induce an immune response against Brucellosis at two inoculations, a higher and a lower dose of ([5 × 10 10 ] and [5 × 10 9 ]) CFU/mL, respectively [ 28 ]. Therefore, the exploration of which conserved protein can effectively protect against Brucella abortus infection in a natural host is essential.…”

The presence of anthracosis is associated with the environmental air quality of zoo, wildlife, and companion animals in Jeollabuk-do Province, South Korea

“…Collected lung tissues were then fixed in 10% neutral buffered formalin and routinely processed for histopathological analysis using a Shandon Citadel 1000 tissue processor. 13,14 Thereafter, paraffin sections of about 5 μm in thickness were prepared using a microtome (HM-340E; Thermo Fisher Scientific). These paraffin sections were placed on glass slides with silane coating for Prussian blue staining using an Iron Stain Kit-IRN-1 (ScyTek Laboratories) following the manufacturer's instructions.…”

Development of new generation of vaccines for Brucella abortus

“…Oral administration of salmonella as vector expressing ribosomal protein L7/L12 and the lumazine synthase enzyme (BLS) resulted in a Th1 type response but failed to protect mice against B. abortus challenge [98]. Immunization of goats with attenuated salmonella vector based vaccine expressing heterologous Brucella antigens (SOD, BLS, prpA and OMP19) induced high levels of IFN-γ and afforded suitable protection efficacy that was comparable with the classic RB51 vaccine [99]. Formulation of this vector with purified Brucella LPS, promoted the efficacy of the delivery when injected ip in mice [100].…”