Vaccination of mice with DNA vaccine induces the immune response and partial protection against T. spiralis infection

Wang, Z.Q.; Cui, Jing; He, Wei; Han, Houxiang; Zhang, H.W.; Li, Yuliang

doi:10.1016/j.vaccine.2005.08.104

Cited by 40 publications

(24 citation statements)

References 23 publications

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“…The procedure was performed as previously described [19]. Briefly, microtiter plates (Nunc) were coated with recombinant TsAP proteins, ES or crude antigens (2.5 μg/ml) in coating buffer overnight at 4°C, and blocked with 200 μl of PBS-0.1% Tween 20 (PBST) containing 5% skimmed milk.…”

Section: Methodsmentioning

confidence: 99%

“…The muscle larvae were examined from another 8 mice from each group 42 days after challenge by artificial digestion of all the whole carcass of each infected mouse [14]. The protective immunity was calculated as the worm reduction rate of recovered adults and larvae per gram (LPG) muscle from the immunized groups versus those from the control groups [19]. …”

Section: Methodsmentioning

confidence: 99%

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Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB/c mice

Zhang

Wang

et al. 2013

Parasites Vectors

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BackgroundTrichinella spiralis is an intracellular parasite that can cause a serious threat to human health by causing trichinellosis. The aminopeptidase (AP) was found in the proteins produced by T. spiralis infective larvae after in vitro co-culture with intestinal epithelial cells, but its characteristics and function are unknown. The purpose of this study was to identify the T. spiralis aminopeptidase (TsAP) and to investigate its potential as a vaccine candidate antigen against T. spiralis infection.MethodsT. spiralis aminopeptidase (TsAP) gene encoding a 54.7 kDa protein was cloned and expressed in Escherichia coli, and purified recombinant TsAP protein was used to immunize BALB/c mice. The antibodies obtained were used to determine where TsAP was localized in the parasite. Transcription and expression of TsAP in different developmental stages of T. spiralis were observed by RT-PCR and Immunofluorescence test (IFT). The immune protection of recombinant TsAP protein against T. spiralis infection in BALB/c mice was evaluated.ResultsAnti-TsAP antibodies recognized the native protein migrating at 54.7 kDa by Western blotting of the crude antigens from muscle larvae. Transcription and expression of TsAP gene was observed in different developmental stages (adult worms, newborn larvae, pre-encapsulated larvae and muscle larvae). TsAP appears to be a cytoplasmic protein located primarily at the cuticle and internal organs of this parasite. After a challenge infection with T. spiralis infective larvae, mice immunized with the recombinant TsAP protein displayed a 38.1% reduction in adult worm burden and 59.1% reduction in muscle larval burden.ConclusionsIn this study, T. spiralis aminopeptidase (TsAP) was first characterized and will help reveal its potential biological functions. TsAP is a novel potential vaccine candidate antigen that merits further investigation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB/c mice

Zhang

Wang

et al. 2013

Parasites Vectors

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“…The 31 kDa E/S antigen of ML (TspE1) and TsNd, an up-regulated gene in IIL compared to ML, have been cloned in pcDNA3.1 vector [53,54]. Recombinant pcDNA3.1-TsNd vaccine conferred higher levels of protection against T. spiralis infection in comparison to pcDNA3.1-TsE1.…”

Section: Third-generation Vaccinesmentioning

confidence: 99%

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Andrade-Becerra¹,

Pompa-Mera²,

MaríaRibas-Aparicio³

et al. 2017

Natural Remedies in the Fight Against Parasites

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Trichinellosis is a food-borne parasitic disease caused by round worms of the genus Trichinella. The majority of human outbreaks are attributed to consumption of raw or undercooked pork meat contaminated with T. spiralis muscle larvae. A blockingtransmission vaccine against trichinellosis will allow preventing swine infection and will contribute to disease control. In this chapter, different vaccine candidates so far developed against T. spiralis, including first-, second-, and third-generation vaccines, are discussed. Most vaccine candidates are based on a unique antigen mainly from the muscle larva stage, inducing with some exceptions, partial protection although a mix Th1/Th2 immune response is elicited. Therefore, the need for identification of new antigens from different parasite stages focusing on infective intestinal larvae, adult, and newborn larvae stages as well as the evaluation of their protective capacity in pigs is presented. The design of multi-epitope vaccines and the use of adjuvants or immunomodulatory molecules capable to polarize the immune response to a Th2-type-protective response are discussed as imperative elements of modern vaccines. Plant-based vaccines and probiotics as excellent tools for vaccine development against T. spiralis are also presented as an attractive platform for veterinary vaccines.

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“…MAbs from the hybridoma culture supernatants and ascites were assayed by an indirect ELISA procedure (Wang et al, 2006). Briefly, each well of polystyrene 96-well plates (Corning, NY) was coated with 100 μL ES antigens of a concentration of 5μg /mL in 0.05M bicarbonate buffer (pH 9.6) and incubated overnight at 4 °C.…”

Section: Elisa Assaymentioning

confidence: 99%

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

Cui

Jing

et al. 2014

Helminthologia

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253 SummaryHybridomas secreting monoclonal antibodies (MAbs) against excretory-secretory (ES) antigen of Trichinella spiralis muscle larvae were produced. The 12 monoclones (designated 3A11, 7D9, 7F6, 5A7, 6H7, 39F3, 38H2, 37E7, 35B9, 29A11, 39C9 and 39A6) secreted IgM, IgG3 and IgG2b, respectively. Western blot using T. spiralis ES antigens showed that ten of 12 MAbs recognized the bands between 119.8 -19.3kDa (mainly 68.4 -28.7kDa) and two MAbs (3A11, 37E7) did not recognize any protein constituents of ES antigens. Nine of 12 MAbs also recognized the larval antigens of T. nativa, T. pseudospiralis and T. nelsoni at 40 days post-infection (dpi). No cross-reactions were found with the somatic antigens of adult worms of P. westermani, C. sinensis, and T. solium cysticercus. However, three (3A11, 6H7 and 39A6) were cross-reacted with the somatic antigens of adult worms of S. japonicum. Immunofluorescent location showed that the nine MAbs reacted with the cuticle and internal organs of T. spiralis larvae. Additionally, five and eight MAbs generated in this study can recognize the early antigens of pre-encapsulated T. spiralis larvae at 11 dpi and 13 dpi, respectively. The generation and characterization of the MAbs against T. spiralis ES antigens provide foundation for the development of specific early serodiagnostic techniques for trichinellosis.

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Vaccination of mice with DNA vaccine induces the immune response and partial protection against T. spiralis infection

Cited by 40 publications

References 23 publications

Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB/c mice

Molecular characterization of Trichinella spiralis aminopeptidase and its potential as a novel vaccine candidate antigen against trichinellosis in BALB/c mice

Vaccination against Trichinella spiralis: Potential, Limitations and Future Directions

Preparation and characterization of monoclonal antibodies against ES antigens of Trichinella spiralis

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