Vaccination strategies to combat nosocomial infections

Venkataraman, Rajesh; Yadav, Umesh C.S.; Shivalingegowda, Ravi Kurikempannadoddi; Shrestha, Yogendra

doi:10.1016/j.vacun.2022.11.001

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…to A. baumannii virulence, but not observed in closely related pathogen A. nosocomialis Acinetobacter species usually exist in the natural environment, such as soils, oceans and freshwater [52]. However, due to its various innate mechanisms, Acinetobacter spp has evolved as an emerging pathogen capable of causing diseases, with A. baumannii having the most clinical significance, which accounts for 80% of hospital-acquired infections [62]. The observed virulence of the selected siderophore production genes prompts whether the identified virulence genes are conserved across other Acinetobacter species capable of causing diseases.…”

Section: High Conservation Of Acinetobactin Baumannoferrin and Isocho...mentioning

confidence: 99%

Transcriptomic Insights into the Virulence of Acinetobacter baumannii During Infection: Role of Iron Uptake and Siderophore Production Genes

Ten,

Rahman,

Tan

2024

Preprint

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Acinetobacter baumanniiis a top-priority pathogen as classified by the World Health Organisation. It causes life-threatening infections in immunocompromised patients, resulting in prolonged hospitalisation and high mortality. Increasing cases of community-acquiredA. baumanniiinfections with rapid progression and severe infections have been reported. This study used the previously describedGalleria mellonellainfection model to investigate the virulence mechanisms of the community strain C98 (Ab-C98) via transcriptomic analysis using direct RNA sequencing. This strain showed greater killing and more rapid colonisation in the larvae than a clinical reference strain (ATCC BAA1605). Differential gene expression analysis revealed the significant upregulation of three major iron clusters: the acinetobactin and baumannoferrin clusters for siderophore production and the Feo system for ferrous iron uptake. Targeted knockout of siderophore production genes (basC,bfnDand isochorismatase family protein) significantly attenuated virulence in mutants with minimal impact on the bacterial growthin vivo. Overall, this study highlights the virulence ofbasC, isochorismatase family protein andbfnDin the pathogenicity ofA. baumannii. As these targets are highly conserved inA. baumanniiand the closely relatedA. pittiiandA. lactucae, they could serve as potential therapeutic targets for developing new antivirulence agents to combat these pathogens.

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Section: High Conservation Of Acinetobactin Baumannoferrin and Isocho...mentioning

confidence: 99%

Transcriptomic Insights into the Virulence of Acinetobacter baumannii During Infection: Role of Iron Uptake and Siderophore Production Genes

Ten,

Rahman,

Tan

2024

Preprint

View full text Add to dashboard Cite

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Transcriptomic insights into the virulence of Acinetobacter baumannii during infection—role of iron uptake and siderophore production genes

Ten,

Rahman,

Tan

2024

FEBS Letters

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Acinetobacter baumannii, a top‐priority WHO pathogen, causes life‐threatening infections in immunocompromised patients, leading to prolonged hospitalisation and high mortality. Here, we used the Galleria mellonella model to investigate community strain C98 (Ab‐C98) virulence via transcriptomic analysis. Ab‐C98 showed greater killing and faster colonisation in larvae than the clinical reference strain (ATCC BAA1605). Genes in three iron clusters, acinetobactin, baumannoferrin and the Feo system, were significantly up‐regulated. Targeted knockout of siderophore genes (basC, bfnD, and the gene encoding isochorismatase) significantly increased the survival of infected larvae by at least 35.16%, identifying these genes as potential targets for developing anti‐virulence agents against A. baumannii.

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Polar solvation molecular dynamics, quantum reactivity (ELF, HOMO–LUMO, NBO) studies, spectroscopy (FT-IR, UV), and the antibiotic potential of carbazide derivative via in-silico molecular docking

Mujafarkani,

Adindu,

Godfrey

et al. 2024

Zeitschrift Für Physikalische Chemie

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Recently, the treatment of bacterial infection has been very worrisome as a decline in antibiotic sensitivity is hitting a majority of the world population. Among many bacterial infection’s causing agents, Enterococcus species and Mycoplasma pneumonia are highly threatening because of their resistance to powerful antibiotics such as vancomycin, erythromycin, and azithromycin. Herein, effect of polar (DMSO, EtOH, MeOH, H2O) solvation on the quantum chemical parameters, molecular structure, spectroscopy, and the antimicrobial potential of p-phenylenediamine-thiosemicarbazide-formaldehyde (PTSF) terpolymer is presented within the framework of density functional theory (DFT), solvation molecular dynamics, and molecular docking approach. Herein, ethanol was keenly observed with the most insightful properties across all analyses specifically by its high energy gap (4.6344 eV) which accounted for the stability of compound. The molecular docking revealed the binding affinities for PSTF with respect to the best docking modes are −4.6 kcal/mol with LYS 288 and bond distance of 2.82 Å for 5V2M and −5.1 kcal/mol with ASN 770 at 2.27 Å, GLU 767 at 2.61 Å, ARG 777 at 3.69 Å, GLU 775 at 2.57 Å, and ALA 763 at 2.86 Å for 6rj1. From the result obtained, the studied compound has higher binding affinity at the active site of 6RJ1 than that of 5V2M. This suggests that PSTF has greater efficacy for inhibiting the growth of M. pneumoniae than vancomycin resistant Enterococcus faecalis. Hence, further in vitro and in vivo studies should delve into the more exploration of the reported ligand.

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Vaccination strategies to combat nosocomial infections

Cited by 3 publications

References 48 publications

Transcriptomic Insights into the Virulence of Acinetobacter baumannii During Infection: Role of Iron Uptake and Siderophore Production Genes

Transcriptomic Insights into the Virulence of Acinetobacter baumannii During Infection: Role of Iron Uptake and Siderophore Production Genes

Transcriptomic insights into the virulence of Acinetobacter baumannii during infection—role of iron uptake and siderophore production genes

Polar solvation molecular dynamics, quantum reactivity (ELF, HOMO–LUMO, NBO) studies, spectroscopy (FT-IR, UV), and the antibiotic potential of carbazide derivative via in-silico molecular docking

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