Vaccination with Heat Shock Protein 60 Induces a Protective Immune Response against Experimental<i>Paracoccidioides brasiliensis</i>Pulmonary Infection

Soares, Renata de Bastos Ascenço; Gómez, Francisco; Soares, Célia Maria de Almeida; Deepe, George S.

doi:10.1128/iai.00753-07

Cited by 53 publications

(28 citation statements)

References 51 publications

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“…Likewise, vaccination of mice against Coccidioides using multiple T-cell epitopes induced resistance, which was linked to induction of Th1 and Th17 cytokines [19]. Several protein-based vaccine candidates have been shown to provide a therapeutic immunity against paracoccidioidomycosis where Th1 and antibodies played a protective role [20–22]. …”

Section: Introductionmentioning

confidence: 99%

Vaccine immunity against fungal infections

Nanjappa

Klein

2014

Current Opinion in Immunology

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Only a handful of the many fungal species on our planet cause infections in humans. Despite many medical advances, invasive fungal infections have skyrocketed in recent years and pose a growing health problem in both immune-competent and -deficient hosts. Recent strides in our understanding of fungal immunity have raised the prospect that vaccines against fungi can be developed that are effective, safe and able to elicit lasting immunity even in immune deficient individuals. We discuss progress in vaccine development and understanding mechanisms of protection against fungi including in patients with impaired CD4+ T-cell immunity.

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Section: Introductionmentioning

confidence: 99%

Vaccine immunity against fungal infections

Nanjappa

Klein

2014

Current Opinion in Immunology

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“…The studies described herein highlight the potential for inducing protection against cryptococcosis in immunocompromised hosts. Previous vaccination strategies using recombinant forms of heat shock protein 60 from H. capsulatum (7) and Paracoccidioides brasiliensis (6) and live H. capsulatum (39), B. dermatitidis, (39,40), and Candida albicans (33) yeast cells have supported the feasibility of developing fungal vaccines for use in patients with severe T cell deficiencies. Each study has enlightened us as to the importance of CD4 ϩ and/or CD8 ϩ T cells during the afferent phase of vaccination and the efferent phase of infection.…”

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confidence: 99%

Protective Immunity against Experimental Pulmonary Cryptococcosis in T Cell-Depleted Mice

Wozniak¹,

Young²,

Wormley³

2011

Clin. Vaccine Immunol.

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Individuals with defects in T cell-mediated immunity (CMI) are highly susceptible to infection with Cryptococcus neoformans. The purpose of these studies was to determine if protection against experimental pulmonary cryptococcosis can be generated in T cell-deficient hosts. BALB/c mice were depleted of CD4 ؉ and/or CD8 ؉ T cells or given an isotype control antibody prior to vaccination with a C. neoformans strain, designated H99␥, previously shown to induce protection against C. neoformans infection in immunocompetent mice. Mice depleted of CD4 ؉ or CD8 ؉ T cells, but not both subsets, survived an acute pulmonary infection with C. neoformans strain H99␥ and a subsequent second challenge with wild-type C. neoformans strain H99. We observed a significant increase in the percentage of CD4 ؉ and CD8 ؉ T cells expressing the activation marker CD69 in the lungs of mice immunized with C. neoformans strain H99␥ prior to a secondary challenge with wild-type cryptococci. CD4؉ T cells within the lungs of immunized mice also appeared to acquire a predominantly activated effector memory cell phenotype (CD69 ؉ CD44 ؉ CCR7 ؊ CD45RB ؊ CD62L ؊ ) following a second pulmonary challenge with wild-type C. neoformans, compared to CD4 ؉ T cells from naïve mice. Lastly, immunization of immunocompetent mice with C. neoformans strain H99␥ prior to depletion of CD4 ؉ and/or CD8 ؉ T cells resulted in significant protection against a second challenge with wild-type C. neoformans. Our studies demonstrate that protective immunity against pulmonary cryptococcosis can be generated in immunosuppressed hosts, thus supporting the development of cryptococcal vaccines.

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“…Consistently, vaccine-induced resistance also correlated with the induction of cell-mediated immunity, with the variable involvement of CD4 + and CD8 + T cells (Figure 2). Th1-mediated protection has been observed with crude antigen preparations from A. fumigatus or recombinant fungal antigens alone [43] or in conjunction with CpG oligonucleotides as adjuvant [19,[44][45][46], mannosylated cryptococcal antigens [47], the Blastomyces adhesin antigen [35], heat shock protein 60 from P. brasiliensis [48], and the multivalent vaccines, comprised complexes of protein antigens of Cocciodiodes, administered in combinations with adjuvants [49,50]. As anticipated, Treg cells, by favoring fungal persistence, may actively contribute to maintenance of antifungal memory [20].…”

Section: Reviewmentioning

confidence: 99%