Vaccine potential of recombinant pro- and mature cathepsinL1 against fasciolosis gigantica in mice

Kueakhai, Pornanan; Changklungmoa, Narin; Chaichanasak, Pannigan; Jaikua, Wipaphorn; Itagaki, Tadashi; Sobhon, Prasert

doi:10.1016/j.actatropica.2015.06.020

Cited by 12 publications

(9 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies have reported the usefulness of chimeric vaccine antigen prepared from FhCatL1 and leucine aminopeptidase (FhLAP) in eliciting an immune response and conferring protection against challenge infection in both rabbit ( 49 ), and sheep ( 81 ). Concerning vaccine researches applied on F. gigantica , immunization of mice with recombinant pro and mature FgCatL1 showed a protective potential against challenge infection when compared with non-immunized control group ( 82 , 83 ).…”

Section: Resultsmentioning

confidence: 98%

High relatedness of bioinformatic data and realistic experimental works on the potentials of Fasciola hepatica and F. gigantica cathepsin L1 as a diagnostic and vaccine antigen

Fereig

Metwally²,

El-Alfy³

et al. 2022

Front. Public Health

View full text Add to dashboard Cite

IntroductionFascioliasis is a parasitic foodborne disease caused by the liver flukes, Fasciola hepatica and F. gigantica. Such parasites cause serious illness in numerous domestic animals and also in humans. Following infection, the parasite secretes a variety of molecules that immediately interact with the host immunity to establish successful infection. These molecules include cathepsin L peptidase 1 (CatL1); the highly investigated diagnostic and vaccine antigens using various animal models. However, a few studies have analyzed the potentials of FhCatL1 as a diagnostic or vaccine antigen using bioinformatic tools and much less for FgCatL1. The present study provides inclusive and exclusive information on the physico-chemical, antigenic and immunogenic properties of F. hepatica cathepsin L1 (FhCatL1) protein using multiple bioinformatic analysis tools and several online web servers. Also, the validation of our employed available online servers was conducted against a huge collection of previously published studies focusing on the properties of FhCatL1as a diagnostic and vaccine antigen.MethodsFor this purpose, the secondary, tertiary, and quaternary structure of FhCatL1 protein were also predicted and analyzed using the SWISS-MODEL server. Validation of the modeled structures was performed by Ramachandran plots. The antigenic epitopes of the protein were predicted by IEDB server.Results and discussionOur findings revealed the low similarity of FhCatL1 with mammalian CatL1, lacking signal peptides or transmembrane domain, and the presence of 33 phosphorylation sites. Also, the containment of FhCatL1 for many topological, physico-chemical, immunological properties that favored its function of solubility and interaction with the immune components were reported. In addition, the earlier worldwide reports documented the high efficacy of FhCatL1 as a diagnostic and vaccine antigen in different animals. Altogether, FhCatL1 is considered an excellent candidate for using in commercialized diagnostic assays or vaccine products against fascioliasis in different animal species. Our assessment also included FgCatL1 and reported very similar findings and outputs to those of FhCatL1.

show abstract

Section: Resultsmentioning

confidence: 98%

High relatedness of bioinformatic data and realistic experimental works on the potentials of Fasciola hepatica and F. gigantica cathepsin L1 as a diagnostic and vaccine antigen

Fereig

Metwally²,

El-Alfy³

et al. 2022

Front. Public Health

View full text Add to dashboard Cite

show abstract

“…Noteworthy, Hanna and Jura [48] described that significantly more F. gigantica flukes successfully established in mice via intraperitoneal injection of NEJ than oral infection with metacercariae. The authors attributed these differences to inappropriate physicochemical conditions for excystment in the gut of the mouse, although the mouse model has been considered suitable for studies on migration and establishment of F. gigantica by several authors (e.g., [49]). Whether these differences are also true for F. hepatica is a matter of future investigations and could contribute to decipher the importance of intestinal passage in the physiology and establishment of F. hepatica in the vertebrate host.…”

Section: Discussionmentioning

confidence: 99%

“…The authors attributed these differences to inappropriate physicochemical conditions for excystment in the gut of the mouse, although the mouse model has been considered suitable for studies on migration and establishment of F . gigantica by several authors (e.g., [ 49 ]). Whether these differences are also true for F .…”

Section: Discussionmentioning

confidence: 99%

Study of the migration of Fasciola hepatica juveniles across the intestinal barrier of the host by quantitative proteomics in an ex vivo model

et al. 2022

View full text Add to dashboard Cite

Fasciola hepatica is a trematode parasite that infects animals and humans causing fasciolosis, a worldwide-distributed disease responsible for important economic losses and health problems. This disease is of growing public health concern since parasite isolates resistant to the current treatment (triclabendazole) have increasingly been described. F. hepatica infects its vertebrate host after ingestion of the encysted parasite (metacercariae), which are found in the water or attached to plants. Upon ingestion, newly excysted juveniles of F. hepatica (FhNEJ) emerge in the intestinal lumen and cross the intestinal barrier, reach the peritoneum and migrate to the biliary ducts, where adult worms fully develop. Despite the efforts made to develop new therapeutic and preventive tools, to date, protection against F. hepatica obtained in different animal models is far from optimal. Early events of host-FhNEJ interactions are of paramount importance for the infection progress in fasciolosis, especially those occurring at the host-parasite interface. Nevertheless, studies of FhNEJ responses to the changing host environment encountered during migration across host tissues are still scarce. Here, we set-up an ex vivo model coupled with quantitative SWATH-MS proteomics to study early host-parasite interaction events in fasciolosis. After comparing tegument and somatic fractions from control parasites and FhNEJ that managed to cross a mouse intestinal section ex vivo, a set of parasite proteins whose expression was statistically different were found. These included upregulation of cathepsins L3 and L4, proteolytic inhibitor Fh serpin 2, and a number of molecules linked with nutrient uptake and metabolism, including histone H4, H2A and H2B, low density lipoprotein receptor, tetraspanin, fatty acid binding protein a and glutathione-S-transferase. Downregulated proteins in FhNEJ after gut passage were more numerous than the upregulated ones, and included the heath shock proteins HSP90 and alpha crystallin, amongst others. This study

show abstract

“…Vaccination is a more cost-effective, sustainable, and environmentally friendly method for controlling the disease and is considered safe for animals and consumers [8]. Previous publications showed that several Fasciola antigens have been tested for the vaccine against fasciolosis, including fatty acid binding protein (FABP) [9], cathepsins B2 and B3 [10], glutathione S-transferase (GST) [11,12], cathepsin L [13][14][15][16], tetraspanin 2 (TSP2) [17], leucine aminopeptidase (LAP) [18][19][20], and saposinlike protein (SAP) [21][22][23][24][25]. Immunizations with recombinant proteins from F. gigantica, including 2-Cys peroxiredoxin (Prx) in mice and buffaloes [26,27], cathepsin L1 in mice, cattle, and sheep [14][15][16], cathepsin L1H in mice [13], cathepsin L1G in mice [28], GST in mice and buffalo [11,12], superoxide dismutase (SOD) in mice [29], cathepsin B2 and B3 [10], LAP in mice [18], SAP-1 in mice [21], and SAP-2 in mice [22] have been shown to protect against F. gigantica infection with a limited outcome.…”

Section: Introductionmentioning

confidence: 99%

Combination Vaccines of Fasciola gigantica Saposin-like Protein-2 and Leucine Aminopeptidase

et al. 2023

Self Cite

View full text Add to dashboard Cite

Saposin-like protein-2 (SAP-2) and leucine aminopeptidase (LAP) are major proteins involved in the digestive process of Fasciola gigantica (Fg). Both SAP-2 and LAP are highly expressed in F. gigantica; therefore, they could be vaccine candidates for fasciolosis. The aims of this study are (1) to observe the tissue expression of F. gigantica SAP-2 (FgSAP-2) and F. gigantica LAP (FgLAP) in F. gigantica by indirect immunofluorescence technique under confocal microscopy and (2) to test the vaccine potentials of individual and combined recombinant (r) FgSAP-2 and rFgLAP against F. gigantica in Imprinting Control Region (ICR) mice (n = 10 per group). By indirect immunofluorescence-confocal microscopy, FgSAP-2 and FgLAP were localized in the caecal epithelium but at different sites: FgSAP-2 appeared in small granules that are distributed in the middle and lower parts of the cytoplasm of epithelial cells, while FgLAP appeared as a line or zone in the apical cytoplasm of caecal epithelial cells. For vaccine testing, the percent protection of combined rFgSAP-2 and rFgLAP vaccines against F. gigantica was at 80.7 to 81.4% when compared with aluminum hydroxide (alum) adjuvant and unimmunized controls, respectively. The levels of IgG1 and IgG2a in the sera were significantly increased in single and combine vaccinated groups compared with the control groups. Vaccinated mice showed reduced liver damage when compared with control groups. This study indicates that the combined rFgSAP-2 and rFgLAP vaccine had a higher vaccine potential than a single vaccine. These results support the further testing and application of this combined vaccine against F. gigantica infection in farmed livestock animals.

show abstract

Vaccine potential of recombinant pro- and mature cathepsinL1 against fasciolosis gigantica in mice

Cited by 12 publications

References 34 publications

High relatedness of bioinformatic data and realistic experimental works on the potentials of Fasciola hepatica and F. gigantica cathepsin L1 as a diagnostic and vaccine antigen

High relatedness of bioinformatic data and realistic experimental works on the potentials of Fasciola hepatica and F. gigantica cathepsin L1 as a diagnostic and vaccine antigen

Study of the migration of Fasciola hepatica juveniles across the intestinal barrier of the host by quantitative proteomics in an ex vivo model

Combination Vaccines of Fasciola gigantica Saposin-like Protein-2 and Leucine Aminopeptidase

Contact Info

Product

Resources

About