Vacuole membrane contact sites and domains: emerging hubs to coordinate organelle function with cellular metabolism

Malia, Pedro Carpio; Ungermann, Christian

doi:10.1042/bst20150277

Cited by 15 publications

(15 citation statements)

References 47 publications

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“…The ER‐mitochondria encounter structure (ERMES) complex and mitochondria‐associated ER membrane (MAM) are contact zones between the mitochondria and ER and they are responsible for the transport of phospholipids and Ca 2+ , respectively. The vacuole and mitochondria patch (vCLAMP) and nuclear–vacuole junction (NVJ) are formed between mitochondria and vacuoles and between the nucleus and vacuoles, respectively, and are responsible for the transfer of membrane lipids. The mitochondrial contact site (MICOS) complex transports phospholipids between the outer and the inner membranes of the mitochondria .…”

Section: Organelle Zones In Organelles Other Than the Golgimentioning

confidence: 99%

Golgi stress response and organelle zones

Sasaki

Yoshida

2019

FEBS Letters

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Organelles have been studied traditionally as single units, but a novel concept is now emerging: each organelle has distinct functional zones that regulate specific functions. The Golgi apparatus seems to have various zones, including zones for: glycosylphosphatidylinositol‐anchored proteins; proteoglycan, mucin and lipid glycosylation; transport of cholesterol and ceramides; protein degradation (Golgi membrane‐associated degradation); and signalling for apoptosis. The capacity for these specific functions and the size of the corresponding zones appear to be tightly regulated by the Golgi stress response to accommodate cellular demands. For instance, the proteoglycan and mucin zones seem to be separately augmented during the differentiation of chondrocytes and goblet cells, respectively. The mammalian Golgi stress response consists of several response pathways. The TFE3 pathway regulates the general function of the Golgi, such as structural maintenance, N‐glycosylation and vesicular transport, whereas the proteoglycan pathway increases the expression of glycosylation enzymes for proteoglycans. The CREB3 and HSP47 pathways regulate pro‐ and anti‐apoptotic functions, respectively. These observations indicate that the Golgi is a dynamic organelle, the capacity of which is upregulated according to cellular needs.

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Section: Organelle Zones In Organelles Other Than the Golgimentioning

confidence: 99%

Golgi stress response and organelle zones

Sasaki

Yoshida

2019

FEBS Letters

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“…The split-GFP system can be used to detect various inter-organelle contact sites. In addition to the ER-mitochondria and mitochondria-vacuole pairs, several different organelle pairs were shown to be in close proximity in yeast cells [18][19][20][21]30 .…”

Section: Resultsmentioning

confidence: 99%

“…A number of proteins have been found to form nuclear-vacuole contact sites called the NVJ (nuclear-vacuole junction) [18][19][20][21] . First, a nuclear membrane protein Nvj1 was found to function as a bridge between the nucleus and vacuole through direct binding to a vacuolar protein Vac8 22 .…”

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confidence: 99%

“…Formation of the NVJ may be critical for selective degradation of a part of the nucleus under a starvation condition 23 . Similar to other organelle contact sites, the NVJ appears to be involved in the lipid homeostasis because several proteins that are known to participate in lipid metabolism are enriched at the NVJ 18,19 . For example, an oxysterol-binding protein (OSBP) Osh1 and a SMP-domain containing protein Nvj2, both of which could function as lipid transfer proteins, are enriched at the NVJ 24,25 .…”

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confidence: 99%

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Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system

Kakimoto

Tashiro

Kojima

et al. 2018

Preprint

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Functional integrity of eukaryotic organelles relies on direct physical contactsbetween distinct organelles. However, the entity of organelle-tethering factors is not well understood due to lack of means to analyze inter-organelle interactions in living cells. Here we evaluate the split-GFP system for visualizing organelle contact sites in vivo and show its advantages and disadvantages. We observed punctate GFP signals from the split-GFP fragments targeted to any pairs of organelles among the ER, mitochondria, peroxisomes, vacuole and lipid droplets in yeast cells, which suggests that these organelles form contact sites with multiple organelles simultaneously although it is difficult to rule out the possibilities that these organelle contacts sites are artificially formed by the irreversible associations of the split-GFP probes. Importantly, split-GFP signals in the overlapped regions of the ER and mitochondria were mainly co-localized with ERMES, an authentic ER-mitochondria tethering structure, suggesting that split-GFP assembly depends on the preexisting inter-organelle contact sites. We also confirmed that the split-GFP system can be applied to detection of the ER-mitochondria contact sites in HeLa cells. We thus propose that the split-GFP system is a

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“…Vacuoles (yeast lysosomes) have many essential roles such as being a hub for ion storage and serving as a final destination for both endosomal and autophagic vesicles [57]. As such, vacuoles have evolved to break-down and recycle a wide variety of macromolecules delivered for degradation.…”

Section: Mitochondria-vacuolesmentioning

confidence: 99%

Mitochatting – If only we could be a fly on the cell wall

Eisenberg-Bord

Schuldiner

2017

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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Mitochondria, cellular metabolic hubs, perform many essential processes and are required for the production of metabolites such as ATP, iron-sulfur clusters, heme, amino acids and nucleotides. To fulfill their multiple roles, mitochondria must communicate with all other organelles to exchange small molecules, ions and lipids. Since mitochondria are largely excluded from vesicular trafficking routes, they heavily rely on membrane contact sites. Contact sites are areas of close proximity between organelles that allow efficient transfer of molecules, saving the need for slow and untargeted diffusion through the cytosol. More globally, multiple metabolic pathways require coordination between mitochondria and additional organelles and mitochondrial activity affects all other cellular entities and vice versa. Therefore, uncovering the different means of mitochondrial communication will allow us a better understanding of mitochondria and may illuminate disease processes that occur in the absence of proper cross-talk. In this review we focus on how mitochondria interact with all other organelles and emphasize how this communication is essential for mitochondrial and cellular homeostasis. This article is part of a Special Issue entitled: Membrane Contact Sites edited by Christian Ungermann and Benoit Kornmann.

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Vacuole membrane contact sites and domains: emerging hubs to coordinate organelle function with cellular metabolism

Cited by 15 publications

References 47 publications

Golgi stress response and organelle zones

Golgi stress response and organelle zones

Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system

Mitochatting – If only we could be a fly on the cell wall

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