Vacuoles and protein bodies

Dashek, William V.; Clore, Amy M.

doi:10.1002/9781118924846.ch12

Cited by 2 publications

(2 citation statements)

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“…The absorbance values were read at 644 and 662 nm in a spectrophotometer against the blank, 80% acetone. Chlorophyll a , chlorophyll b , and total chlorophyll contents were calculated as milligram of chlorophyll per gram of leaf tissue using the following equations: Chlorophyll a : [9.78 × (A 662) − 0.99 × (A 644)] × V × V 2 × 100/w ×V 1 × 1000 Chlorophyll b : [21.426 × (A 644) − 4.65 × (A 662)] × V × V 2 × 100/w × V 1 × 1000 where A = absorbance at specific wavelength, V = volume of the initial chlorophyll extract, V 1 = volume of the initial chlorophyll extract, which was taken to dilute, V 2 = volume of the diluted chlorophyll extract, V = and w = fresh weight of the tissue extracted [ 90 ].…”

Section: Methodsmentioning

confidence: 99%

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Impact of Wheat Streak Mosaic Virus on Peroxisome Proliferation, Redox Reactions, and Resistance Responses in Wheat

Mіshchеnkо

Nazarov

Dunich

et al. 2021

IJMS

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Although peroxisomes play an essential role in viral pathogenesis, and viruses are known to change peroxisome morphology, the role of genotype in the peroxisomal response to viruses remains poorly understood. Here, we analyzed the impact of wheat streak mosaic virus (WSMV) on the peroxisome proliferation in the context of pathogen response, redox homeostasis, and yield in two wheat cultivars, Patras and Pamir, in the field trials. We observed greater virus content and yield losses in Pamir than in Patras. Leaf chlorophyll and protein content measured at the beginning of flowering were also more sensitive to WSMV infection in Pamir. Patras responded to the WSMV infection by transcriptional up-regulation of the peroxisome fission genes PEROXIN 11C (PEX11C), DYNAMIN RELATED PROTEIN 5B (DRP5B), and FISSION1A (FIS1A), greater peroxisome abundance, and activation of pathogenesis-related proteins chitinase, and β-1,3-glucanase. Oppositely, in Pamir, WMSV infection suppressed transcription of peroxisome biogenesis genes and activity of chitinase and β-1,3-glucanase, and did not affect peroxisome abundance. Activity of ROS scavenging enzymes was higher in Patras than in Pamir. Thus, the impact of WMSV on peroxisome proliferation is genotype-specific and peroxisome abundance can be used as a proxy for the magnitude of plant immune response.

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Section: Methodsmentioning

confidence: 99%

“…where A = absorbance at specific wavelength, V = volume of the initial chlorophyll extract, V 1 = volume of the initial chlorophyll extract, which was taken to dilute, V 2 = volume of the diluted chlorophyll extract, V = and w = fresh weight of the tissue extracted [90].…”

Section: Biochemical Assaysmentioning

confidence: 99%