In a recent paper [1] using a conjecture it is shown how one can calculate the effect of a weak stationary gravitational field on vacuum energy in the context of Casimir effect in an external gravitational field treated in 1 + 3 formulation of spacetime decomposition.. In this article, employing quantum field theory in curved spacetime, we explicitly calculate the effect of a weak static gravitational field on virtual massless scalar particles in a Casimir apparatus. It is shown that, as expected from the proposed conjecture, both the frequency and renormalized energy of the virtual scalar field are affected by the gravitational field through its index of refraction. This could be taken as a strong evidence in favour of the proposed conjecture. Generalizations to weak stationary spacetimes and virtual photons are also discussed. * Electronic address: bornadel@khayam.ut.ac.ir † Electronic address: nouri@theory.ipm.ac.ir, corresponding author 1