Vacuum-assisted delivery has been associated with several complications, including rare vesicular eruptions. Herpes simplex virus has been cultured in a substantial portion of these cases. A case of vesicular eruption from vacuum-assisted delivery is presented in a 1-day-old neonate. The patient was placed on empiric acyclovir until viral cultures and polymerase chain reaction for herpes simplex virus were found to be negative. The lesions resolved without sequelae. The vesicular eruption of vacuum-assisted delivery is probably secondary to mechanical trauma. Clinicians should be aware of the possible risk of herpes simplex virus infection, instituting antiviral therapy until this is excluded.