Vacuum-Packaged Suspended Microchannel Resonant Mass Sensor for Biomolecular Detection

Burg, Thomas P.; Mirza, Asif; Milović, Nebojša M.; Tsau, Christine H.; Popescu, Gabriel; Foster, John S.; Manalis, Scott R.

doi:10.1109/jmems.2006.883568

Cited by 139 publications

(95 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…without compromising overall performance [26]. The device used throughout this work is already compatible with the volumes and flow rates typically available in microfluidics, and with appropriate integration, similar results should be attainable in the context of detection in gTAS.…”

Section: Resultsmentioning

confidence: 81%

Suspended Microchannel Resonators for Ultralow Volume Universal Detection

et al. 2008

Self Cite

View full text Add to dashboard Cite

Universal detectors that maintain high sensitivity as the detection volume is reduced to the sub-nanoliter scale can enhance the utility of miniaturized total analysis systems (ji-TAS). Here the unique scaling properties of the suspended microchannel resonator (SMR) are exploited to show universal detection in a 10 pL analysis volume with a density detection limit of -1 [gg/cm 3 (10 Hz bandwidth) and a linear dynamic range of six decades. Analytes with low UV extinction coefficients such as polyethylene glycol (PEG) 8 KDa, glucose, and glycine are measured with molar detection limits of 0.66 RM, 13.5 gM, and 31.6 JIM, respectively. To demonstrate the potential for real-time monitoring, gel filtration chromatography was used to separate different molecular weights of PEG as the SMR acquired a chromatogram by measuring the eluate density. This work suggests that the SMR could offer a simple and sensitive universal detector for various separation systems from liquid chromatography to capillary electrophoresis. Moreover, since the SMR is itself a microfluidic channel, it can be directly integrated into g-TAS without compromising overall performance. AcknowledgementsFirst of all, I wish to thank Professor Scott Manalis for the opportunity to do my thesis research in his laboratory. Scott has given me invaluable guidance that fosters creativity, teamwork, enthusiasm, and handson research. I also want to thank all group members who contributed to this work: Thomas Burg originally developed the SMR and related setups which I used for my research inline with HPLC system. He also gave me countless advices regarding experiment. Will Grover was an indispensable resource for everything from the knowledge involving HPLC to experimental details. Michel Godin was a good consultant who gave me an insight to research in general. Scott Knudsen helped me dealing with problems involving chemistry and biology with great passion. Yao-Chung (Wesley) Weng gave me a great help in dealing with electronics such as oscillating circuits. Furthermore, the passion of Rumi Chunara, diligence of Andrea Bryan, optimism of Marcio von Muhlen, and discretion of Phillp Dextras has been constant sources of motivation for me. I also thank Ken Babcock and Alison Skelley for valuable discussions.

show abstract

Section: Resultsmentioning

confidence: 81%

Suspended Microchannel Resonators for Ultralow Volume Universal Detection

et al. 2008

Self Cite

View full text Add to dashboard Cite

show abstract

“…23 The Autosampler Chip automates biological measurements by routing samples, reagents, and cleaning solutions to and from a sensor, in this case a Suspended Microchannel Resonator or SMR mass sensor. 24,25 Preliminary experiments show that the FEP Teflon valves in the Autosampler Chip are capable of delivering piranha solution (concentrated sulfuric acid with hydrogen peroxide) to the SMR to strip bound layers from the silica surface of the sensor and reset it to a likenew state. While concentrated sulfuric acid dissolves PDMS, 6 exposure to piranha appears to have no detectable effect on the appearance or operation of the FEP valves in the Autosampler Chip.…”

Section: Discussionmentioning

confidence: 99%

Teflon films for chemically-inert microfluidic valves and pumps

2008

Self Cite

View full text Add to dashboard Cite

We present a simple method for fabricating chemically-inert Teflon microfluidic valves and pumps in glass microfluidic devices. These structures are modeled after monolithic membrane valves and pumps that utilize a featureless polydimethylsiloxane (PDMS) membrane sandwiched between two etched glass wafers. The limited chemical compatibility of PDMS has necessitated research into alternative materials for microfluidic devices. Previous work has shown that spincoated amorphous fluoropolymers and Teflon-fluoropolymer laminates can be fabricated and substituted for PDMS in monolithic membrane valves and pumps for space flight applications. However, the complex process for fabricating these spin-coated Teflon films and laminates may preclude their use in many research and manufacturing contexts. As an alternative, we show that commercially-available fluorinated ethylene-propylene (FEP) Teflon films can be used to fabricate chemically-inert monolithic membrane valves and pumps in glass microfluidic devices. The FEP Teflon valves and pumps presented here are simple to fabricate, function similarly to their PDMS counterparts, maintain their performance over extended use, and are resistant to virtually all chemicals. These structures should facilitate lab-on-a-chip research involving a vast array of chemistries that are incompatible with native PDMS microfluidic devices.

show abstract

“…Frequently employed in mechanical systems, modal methods are the basis of both dynamic atomic force microscopy (dAFM) and micro-cantilever based sensing. For these systems, accurate measurements of frequency shifts of vibrating microcantilevers are the basis of successful devices [2][3][4]. Continued efforts to improve these and related technologies attest to the general effectiveness of modal methods.…”

Section: Introductionmentioning

confidence: 99%

Sensitivity vector fields in time-delay coordinate embeddings: Theory and experiment

Sloboda

Epureanu

2013

Phys. Rev. E

View full text Add to dashboard Cite

Identifying changes in the parameters of a dynamical system can be vital in many diagnostic and sensing applications. Sensitivity vector fields (SVFs) are one way of identifying such parametric variations by quantifying their effects on the morphology of a dynamical system's attractor. In many cases, SVFs are a more effective means of identification than commonly employed modal methods. Previously, it has only been possible to construct SVFs for a given dynamical system when a full set of state variables is available. This severely restricts SVF applicability because it may be cost prohibitive, or even impossible, to measure the entire state in high-dimensional systems. Thus, the focus of this paper is constructing SVFs with only partial knowledge of the state by using time-delay coordinate embeddings. Local models are employed in which the embedded states of a neighborhood are weighted in a novel way referred to as embedded point cloud averaging. Application of the presented methodology to both simulated and experimental time series demonstrates its utility and reliability.

show abstract

Vacuum-Packaged Suspended Microchannel Resonant Mass Sensor for Biomolecular Detection

Cited by 139 publications

References 31 publications

Suspended Microchannel Resonators for Ultralow Volume Universal Detection

Suspended Microchannel Resonators for Ultralow Volume Universal Detection

Teflon films for chemically-inert microfluidic valves and pumps

Sensitivity vector fields in time-delay coordinate embeddings: Theory and experiment

Contact Info

Product

Resources

About