Vaginal and Oral Epithelial Cell Anti-CandidaActivity

Oral and vaginal epithelial cell anti‐Candida activity is acid labile and does not require live epithelial cells

“…Despite the effective cleavage of sulfated polysaccharides, sialic acid residues, or glucose-and mannose-containing carbohydrates, a specific carbohydrate moiety was not identified (10,14).…”

“…Studies to date show that vaginal and oral epithelial cell anti-Candida activity requires cell contact and a putative carbohydrate moiety (10,14). Although a specific effector carbohydrate had yet to be identified, the role for a carbohydrate comes from the sensitivity of the antifungal activity to periodic acid that cleaves carbohydrates to aldehydes and thus releases the majority of the sugar moieties from the cell surface.…”

“…Mouse vaginal epithelial cells were isolated as previously described (10). Briefly, vaginae from sacrificed mice were excised and placed into a sterile glass Petri dish containing 10 ml of dispase I-neutral protease (0.25 mg/ml; Roche Diagnostics, Mannheim, Germany) and incubated on a shaking platform (speed, 110 r.p.m.)…”

“…Following the incubation, the cells were washed with PBS and cell viability was assessed by Trypan blue dye exclusion and confirmed by propidium iodide (PI) and fluorescein diacetate (FDA) vital staining. For PI/FDA staining, FDA (50 μg/ml, stains live cells) and PI (1 μg/ml, stains dead cells) (Sigma) (3,8,10) were added simultaneously to the epithelial cell pellets and placed in the dark for 30 min at room temperature. Following the incubation, the epithelial cells were washed with PBS followed by a second wash with 20% FBS and then a final wash with PBS.…”

The New About Congenital Antimicrobial Defense of Some Epithelial Tissues – Vaginal Mucosa and Hair

“…In VVC most important are reputed the factors of local immunity, among which listed phagocytes and epithelial cells (Nomanbhoy et al, 2002), antibodies (Barousse et al, 2004), cytokines and interferon (Shabashova et al, 2006), concentration of lactate and pH, as well as antimicrobial peptides (AMP) secreted by different leucocytes and epithelial cells (Valore et al, 2002(Valore et al, , 2006.…”