Vaginal carriage and antibiotic susceptibility profile of group B Streptococcus during late pregnancy in Ismailia, Egypt

Abstract

Group B Streptococcus (GBS) infection has long been recognized as a frequent cause of morbidity and mortality in newborn infants. The purpose of this study was to determine the colonization rate with GBS and the antibiotic susceptibility profile in pregnant women attending Gynecological clinics in Egypt. One-hundred and fifty vaginal swabs were collected from pregnant women at 35-40 weeks of gestation. In comparison to culture, direct latex agglutination testing revealed 100% sensitivity and 93.75% specificity. Thirty-eight specimens (25.3%) were found to be positive for GBS. Each isolate was tested for susceptibility to penicillin G, ampicillin, cefotaxime, erythromycin, clindamycin and vancomycin. Erythromycin-resistant isolates were further classified by double-disk method. All isolates were susceptible to penicillin G, ampicillin and vancomycin. Resistance to cefotaxime was detected in three isolates (7.89%). Five isolates (13.15%) were resistant to erythromycin and nine isolates (23.68%) were resistant to clindamycin. Four (80%) isolates had constitutive macrolide-lincosamide-Streptogramin(B) resistance (cMLSB(B)) resistance and one (20%) isolate had inducible resistance (iMLS(B)) resistance. GBS colonization was found to be high in our region. Latex agglutination testing and Islam medium are reliable methods to detect GBS in late pregnancy; however, latex agglutination test is rapid and simpler. Penicillin G remains the first choice antibiotic for treatment of GBS infections.

Group B Streptococcus Carriage during Late Pregnancy in Ile-Ife, Nigeria

“…In this study, there were 3 likely false positive and no false negative cases showing that antigen detection had a sensitivity of 100% and a specificity of 95.6% with positive predictive value of 85% and negative predictive value of 100%. In this study, we recorded a higher prevalence of 11.3% GBS vaginal colonization than that reported in Jos (6.6%) (9) and Calabar(9%) (19) but lower than that reported in Ibadan (17.6%) (5) and Zaria (14%) (8). The prevalence rate recorded is comparable to 11% prevalence obtained in Abeokuta (20) and the recent study carried out in Ibadan (10%) (21).…”

“…One hundred and fifty (150) pregnant women participated in the study over a period of six months (May to December 2010). Their age range was 18-42 years with a mean of 30.25 (±4.65) and the age group in years was categorized as younger (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) and older (≥30) pregnant women, which were 64 (42.7%) and 86 (57.3%) respectively. Table 1 shows parity and gestational age among other parameters as indicated.…”

“…Among the subjects that were colonized with GBS, GBS colonization was higher among older women with age ≥30 years (12.79%) against 9.38% in younger (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29) women (Table 3). The difference was not statistically significant (X 2 = 0.426; P= 0.514).…”

Aumento de resistencia de Streptococcus agalactiae vaginal-anal en el tercer trimestre de gestación a eritromicina y clindamicina al cabo de una década de tamizaje universal

“…Al-Sweih y cols (año 2003), describieron un perfi l de susceptibilidad antimicrobiana en un total de 1.166 muestras vaginales-rectales obteniendo 1,7% 11,12 , encontraron un aumento importante de resistencia a clindamicina y eritromicina: 62 y 65%, respectivamente. En los últimos tres años, regiones tan diferentes como Australia y Malasia muestran resistencias relativamente bajas, tanto a clindamicina (4,2 y 2,5%) como a eritromicina (6,4 y 4%); en cambio, desde Egipto y Tanzania se han comunicado resistencias más elevadas, y que están más en concordancia con lo que sucede en nuestra experiencia [13][14][15][16] .…”

Maternal-child health

“…In rural Zimbabwe, GBS prevalence was as high as 60% whereas in urban Zimbabwe it was 46% (Mavenyengwa et al, 2006). In Egypt, GBS prevalence was 25% (Shabayek et al, 2009). Global GBS prevalence is estimated to be around 20%.…”