Vaginal epithelium and microflora characteristics in women with premature ovarian failure under hormone therapy compared to healthy women

Benetti‐Pinto, Cristina Laguna; Giraldo, Paulo César; Pacello, Poliana Cordeiro César; Soares, Patrícia Magda; Yela, Daniela Angerame

doi:10.1007/s00404-015-3616-2

Cited by 13 publications

(12 citation statements)

References 23 publications

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“…We found the vaginal microbiota to be increasingly diverse with increased species richness in the case of POF, and a significant shift in overall microbial diversity was observed. However, a previous cross-sectional study of microbiota failed to identify obvious differences between individuals in terms of vaginal microbiota diversity [28,29]. The strength of our study lies in the comprehensive description of microbial communities associated with POF achieved through the use of 16S rRNA sequencing; particularly, the association with clinical characteristics of POF; and the utilisation of predictive models to identify bacterial taxa that are differentially expressed in POF.…”

Section: Discussionmentioning

confidence: 86%

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Changes in the vaginal microbiota associated with primary ovarian failure

Wang

Han

et al. 2020

Preprint

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BackgroundPrimary ovary failure (POF) is defined as follicular failure in women of reproductive age. Although many factors are speculated to contribute to the occurrence of POF, the exact aetiology is unclear.Alterations in the microbiome of women with POF are poorly studied; MethodsThis study investigated the vaginal microbiota of 22 patients with POF and 29 healthy individuals.High-throughput Illumina Miseq sequencing targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene was used to evaluate the relationships between vaginal flora and clinical characteristics of POF. ResultsDifferent from before, we found the diversity and richness of the vaginal flora of patients with POF was significantly different to that of healthy controls. Comparison of the flora of patients POF with that of menopausal women revealed that the relative abundance of Lactobacillus was significantly reduced in the latter, and women with reduced relative abundance of Lactobacillus in microbiota community decreased the pregnancy success rate at term. This study confirms that vaginal microbiota dysbiosis occurs in patients with POF. Additionally, the vaginal microbiota is closely related to clinical characteristics of POF and the inclusion of a disease verification model could reveal more accurate information related to the composition of the microbiota and its functions. ConclusionThe result suggests the present study enabled identification of microbiota associated with POF, further investigations of differences in the microbiota in the context of POF will improve our understanding of the pathogenesis of the disease which involve modification of the vaginal microbiota.

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Section: Discussionmentioning

confidence: 86%

“…Previous studies on the vaginal microbiota in the case of POF have mainly involved amine test (or the Whiff test) [28], whereas our study focused on differences at the level of the genus. One of the most attractive features of 16S rRNA gene sequence informatics is the potential for genus identification [30].…”

Section: Discussionmentioning

confidence: 99%

Changes in the vaginal microbiota associated with primary ovarian failure

Wang

Han

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…We found the vaginal microbiota to be increasingly diverse with increased species richness in the case of POF, and a significant shift in overall microbial diversity was observed. However, a previous cross-sectional study of microbiota failed to identify obvious differences between individuals in terms of vaginal microbiota diversity [34,35]. The strength of our study lies in the comprehensive description of microbial communities associated with POF achieved through the use of 16S rRNA sequencing; particularly, the association with clinical characteristics of POF; and the utilisation of predictive models to identify bacterial taxa that are differentially expressed in POF.…”

Section: Discussionmentioning

confidence: 86%

“…Previous studies on the vaginal microbiota in the case of POF have mainly involved amine test (or the Whiff test) [34], whereas our study focused on differences at the level of the genus. One of the most attractive features of 16S rRNA gene sequence informatics is the potential for genus identification [36].…”

Section: Discussionmentioning

confidence: 99%

Changes in the vaginal microbiota associated with primary ovarian failure

Wang

Han

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Primary ovary failure (POF) is defined as follicular failure in women of reproductive age. Although many factors are speculated to contribute to the occurrence of POF, the exact aetiology is unclear. Alterations in the microbiome of women with POF are poorly studied; Methods: This study investigated the vaginal microbiota of 22 patients with POF and 29 healthy individuals. High-throughput Illumina Miseq sequencing targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene was used to evaluate the relationships between vaginal flora and clinical characteristics of POF. Results: Different from before, we found that the diversity and richness of the vaginal flora of patients with POF was significantly different from that of healthy controls. Comparison of the flora of patients with POF with that of menopausal women revealed that the relative abundance of Lactobacillus was significantly reduced in the latter, and women with reduced relative abundance of Lactobacillus in microbiota community decreased the pregnancy success rate at term. The result suggests the present study enabled identification of microbiota associated with POF, further investigations of differences in the microbiota in the context of POF will improve our understanding of the pathogenesis of the disease which involve modification of the vaginal microbiota. Conclusion: The present study identified the microbiota associated with POF. Further investigations on the differences in the microbiota in the context of POF will improve our understanding of the pathogenesis of the disease which involves modification of the vaginal microbiota.

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“…Bentti-Punto et al (2015) studied the effect of estrogen replacement therapy on the microflora and trophic state of vaginal epithelium, sexual function of women with early menopause, comparing them with women with persistent menstrual function. It has been shown that despite the absence of significant differences in the composition of the microbial landscape, the frequency of fungal infection, vaginal pH, women receiving hormone replacement therapy due to premature termination of ovarian function have a lower quality of sex life than their peers, mainly due to pain and decreased moisture [17].…”

Section: Menopausal Symptomsmentioning

confidence: 99%