Vaginal reconstruction by skin grafts: A scanning electron microscopic evaluation

Barberini, Fabrizio; Vizza, Enrico; Montanino, M.; Marcoccia, Sante; Montanino, G

doi:10.1016/s0940-9602(11)80314-6

Cited by 12 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, earlier studies have shown that neovagina created with skin grafts maintain a keratinized epithelium with sebaceous glands and hairs many years after the operation (15). In comparison, we believe that an advantage of our technique is the possibility of obtaining a neovaginal mucosa that is very similar to the normal vaginal mucosa.…”

Section: Discussionmentioning

confidence: 79%

Laparoscopically assisted uterovestibular anastomosis in patients with uterine cervix atresia and vaginal aplasia

Fedele

Bianchi

Frontino

et al. 2008

Fertility and Sterility

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 79%

Laparoscopically assisted uterovestibular anastomosis in patients with uterine cervix atresia and vaginal aplasia

Fedele

Bianchi

Frontino

et al. 2008

Fertility and Sterility

View full text Add to dashboard Cite

“…However, most studies on the application of these materials have been case reports, and all these materials (except skin and the peritoneum) require the application of allografts. Further, a neovagina that uses skin as a covering can still maintain keratinized epithelial cells, sebaceous glands, and hair even 2 years after the operation ( 29 ). In our study, a peritoneal flap was applied instead of other allografts or skin grafts, which might help to reduce the additional damage to the uterus and decrease the expense of surgery.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and safety of laparoscopic vaginoplasty using the peritoneal flap and cervicoplasty in patients with congenital cervical and complete vaginal atresia: a pilot study

Liu¹,

Zhang²,

Wang³

et al. 2023

Ann Transl Med

View full text Add to dashboard Cite

Background: Hysterectomy places a huge physical and mental burden on young female patients with congenital cervical and complete vaginal atresia. Thus, it is necessary to develop a method to detach the obstruction and simultaneously preserve the vagina and uterus in these patients. This study sought to evaluate the efficacy and safety of laparoscopic vaginoplasty using the peritoneal flap and cervicoplasty in patients with congenital cervical and complete vaginal atresia.Methods: Between April 2013 and June 2022, 9 patients with congenital cervical and complete vaginal atresia at Henan Provincial People's Hospital were enrolled in this prospective study. All the patients were treated with laparoscopic vaginoplasty using the peritoneal flap and cervicoplasty. Baseline clinical features (such as age, uterus size, etc.) were collected. The surgical success rate and adverse events were assessed. Results:The 9 enrolled patients had a median [interquartile range (IQR)] age of 15.0 (14.0-18.0) years, and 5/9 patients presented with pelvic adhesions. The surgeries were successful in all (9/9) patients, who preserved their vagina and uterus with a normal menstrual cycle. After a median follow-up duration of 48 months, the neovagina had a median length of 7.5 cm. Post-surgical complications occurred in 3/9 patients, which were cured by an appropriate treatment. The 5/9 married patients reported being satisfied with their sexual life.Conclusions: Even though the current study preliminary exhibits the efficiency of laparoscopic vaginoplasty using the peritoneal flap and cervicoplasty in patients with congenital cervical and complete vaginal atresia, due to the small sample size, lack of a control group, and relatively high incidence of the adverse events, further studies are still needed to verify the current findings. The current study put forward a further direction for preserving the vagina and uterus simultaneously for those patients with congenital cervical and complete vaginal atresia.

show abstract

“…These cells can provide both structural and immunological defense mechanisms against STI pathogens. In vivo, vaginal ECs are characterized as sheets of multicellular stratified squamous epithelia that exhibit tissue-specific protein expression patterns between the basal and suprabasal surfaces, produce mucus glycoproteins that aid in enzymatic and pathological defense, and exhibit distinct morphological features like microfolds, microridges, and cell-cell adhesion structures [3][4][5][6][7]. These ECs also play a critical role in immunological response to pathogens and pathogen-associated molecular patterns (PAMPs) through toll-like receptor (TLR) signaling cascades, cytokine and chemokine production, as well as immune cell recruitment and activation [1,2,8,9].…”

Section: Introductionmentioning

confidence: 99%

Development and Characterization of a Three-Dimensional Organotypic Human Vaginal Epithelial Cell Model1

Hjelm

Berta

Nickerson

et al. 2010

Biology of Reproduction

View full text Add to dashboard Cite

We have developed an in vitro human vaginal epithelial cell (EC) model using the innovative rotating wall vessel (RWV) bioreactor technology that recapitulates in vivo structural and functional properties, including a stratified squamous epithelium with microvilli, tight junctions, microfolds, and mucus. This three-dimensional (3-D) vaginal model provides a platform for high-throughput toxicity testing of candidate microbicides targeted to combat sexually transmitted infections, effectively complementing and extending existing testing systems such as surgical explants or animal models. Vaginal ECs were grown on porous, collagen-coated microcarrier beads in a rotating, low fluid-shear environment; use of RWV bioreactor technology generated 3-D vaginal EC aggregates. Immunofluorescence and scanning and transmission electron microscopy confirmed differentiation and polarization of the 3-D EC aggregates among multiple cell layers and identified ultrastructural features important for nutrient absorption, cell-cell interactions, and pathogen defense. After treatment with a variety of toll-like receptor (TLR) agonists, cytokine production was quantified by cytometric bead array, confirming that TLRs 2, 3, 5, and 6 were expressed and functional. The 3-D vaginal aggregates were more resistant to nonoxynol-9 (N-9), a contraceptive and previous microbicide candidate, when compared to two-dimensional monolayers of the same cell line. A dose-dependent production of tumor necrosis factor-related apoptosis-inducing ligand and interleukin-1 receptor antagonist, biomarkers of cervicovaginal inflammation, correlated to microbicide toxicity in the 3-D model following N-9 treatment. These results indicate that this 3-D vaginal model could be used as a complementary tool for screening microbicide compounds for safety and efficacy, thus improving success in clinical trials.

show abstract

Vaginal reconstruction by skin grafts: A scanning electron microscopic evaluation

Cited by 12 publications

References 6 publications

Laparoscopically assisted uterovestibular anastomosis in patients with uterine cervix atresia and vaginal aplasia

Laparoscopically assisted uterovestibular anastomosis in patients with uterine cervix atresia and vaginal aplasia

Efficacy and safety of laparoscopic vaginoplasty using the peritoneal flap and cervicoplasty in patients with congenital cervical and complete vaginal atresia: a pilot study

Development and Characterization of a Three-Dimensional Organotypic Human Vaginal Epithelial Cell Model1

Contact Info

Product

Resources

About