Vagococcus allomyrinae sp. nov. and Enterococcus larvae sp. nov., isolated from larvae of Allomyrina dichotoma

Kim, Sung-Min; Byeon, Yeong-Sik; Yang, Hong; Kim, In Seop; Lee, Soon Dong

doi:10.1099/ijsem.0.005382

Cited by 11 publications

(2 citation statements)

References 37 publications

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“…Seven of these animal-associated Vagococcus were cultured from animals living in water. Four Vagococcus type species have been isolated from insects: Vagococcus allomyrinae , Vagococcus entomophilus , Vagococcus coleopterorum and Vagococcus hydrophili from, respectively, the Japanese rhinoceros beetle Allomyrina dichotoma [7], wasp Vespula vulgaris [8] and the diving beetles Cybister lewisianus and Hydrophilus acuminatus , respectively [9].…”

Section: Introductionmentioning

confidence: 99%

Vagococcus luciliae sp. nov., isolated from the common green bottle fly Lucilia sericata

Guzman

Daniel

Kämpfer

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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The genus Vagococcus belongs to the family Enterococcaceae (order Lactobacillales) and is closely related to the genus Enterococcus . Currently, 19 species of the genus have been validly named. In this study, we isolated strain G314FT from the common green bottle fly Lucilia sericata collected in Germany. Sequencing of its almost-complete 16S rRNA gene revealed that the isolate belongs to the genus Vagococcus , being closely related to Vagococcus bubulae SS1994T with high sequence identity (99.50 %), followed by Vagococcus martis D7T301T (98.86 %), Vagococcus vulneris SS1995T (98.71 %), Vagococcus teuberi DSM 21459T (98.64 %), Vagococcus silagei 2B-2T (98.64 %) and Vagococcus penaei CD276T (98.64 %). Genome sequencing of strain G314FT was performed by a combination of Illumina and Oxford Nanopore technology, yielding a circular genome with a size of 2 139 468 bp and an 11 kb plasmid. Average nucleotide identity and digital DNA–DNA hybridization values were calculated between G314FT and its closest-related taxa, and found to be <91 % and <40 %, respectively, thus strongly supporting that strain G314FT represents a novel species of the genus Vagococcus . Phylogenetic and core protein-based phylogenomic trees revealed that G314FT was closely related to a group of three species, V. bubulae SS1994T, V. martis D7T301T and V. teuberi DSM 21459T. Comparatively, the genome of G314FT is the smallest in the group of the four related species, and the biochemical pathway comparison using BlastKOALA revealed that G314FT has lost some amino acid biosynthetic proteins; however, it has gained enzymes for carbohydrate metabolism. Phenotypically, G314FT was consistent with other species of the genus Vagococcus including a negative catalase reaction and non-motility. Using the polyphasic approach, our data supports that the isolate represents a new species, for which we propose the name Vagococcus luciliae G314FT (=DSM 112651T= CCM 9164T).

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Section: Introductionmentioning

confidence: 99%

Vagococcus luciliae sp. nov., isolated from the common green bottle fly Lucilia sericata

Guzman

Daniel

Kämpfer

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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“…They have also been detected in livestock-associated environments [10][11][12] and foods containing animal products [3,[13][14][15]. The other sources of isolation have included grain [16], bioreactors [17] and insects [18,19]. Several Vagococcus species have been reported harmful to their host.…”

Section: Introductionmentioning

confidence: 99%

Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat

Johansson

Jääskeläinen

Säde

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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During our studies on spoilage microbiomes of modified atmosphere packaged broiler meat, we isolated three strains (PNs007T, STAA11T and STAA25) of unknown identity. In this present polyphasic taxonomy study, including genome-based analyses, we discovered that these isolates represent two novel species belonging to the genus Vagococcus . In all phylogenetic analyses, PNs007T was positioned very close to Vagococcus fessus but both the average nucleotide identity (ANI; 89.5 %) and digital DNA–DNA hybridization (dDDH; 38.3 %) values distinguished it as a novel vagococcal species. STAA11T and STAA25 were genetically highly similar (16S rRNA, ANI and dDDH 100 %). The phylogenetic position of STAA11T was adjacent to but out of the cluster containing V. fessus , Vagococcus coleopterorum and PNs007T. According to the ANI (76.2–76.4 %) and dDDH (<22.6 %) values it also represented a novel vagococcal species. Phenotypic characteristics and chemotaxonomic properties of both novel species were typical for vagococci and they contained C16 : 0 (25.5–30.1 %) and C18 : 1 ω9c (67.3–73.0 %) as the major cellular fatty acids. The streptomycin-resistant genotype of STAA11T and STAA25 allowing the growth on streptomycin thallous acetate actidione medium was considered to result from a modification in codon 104 of the rpsL gene leading to P104A substitution. The ability of STAA11T and STAA25 to produce ammonia from arginine separated them from PNs007T, which did not show arginine deiminase activity. We propose the names Vagococcus proximus sp. nov. (type strain PNs007T=DSM 115185T=CCUG 76696T) and Vagococcus intermedius sp. nov. (type strain STAA11T=DSM 115183T=CCUG 76697T) for these novel species.

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Enterococci as a One Health indicator of antimicrobial resistance

Zaidi,

Zaheer,

Zovoilis

et al. 2024

Can. J. Microbiol.

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The rapid increase of antimicrobial resistant bacteria in humans and livestock is concerning. Antimicrobials are essential for the treatment of disease in modern day medicine and their misuse in humans and food animals has contributed to an increase in the prevalence of antimicrobial resistant bacteria. Globally, antimicrobial resistance is recognized as an One-health problem affecting humans, animals and environment. Enterococcal species are gram positive bacteria that are widely distributed in nature. Their occurrence, prevalence and persistence across the One-health continuum make them an ideal candidate to study antimicrobial resistance from a One-health perspective. The objective of this review was to summarize the role of enterococci as an indicator of antimicrobial resistance across One-health sectors. We also briefly address the prevalence of enterococci in human, animal and environmental settings. In addition, a 16S RNA gene based phylogenetic tree was constructed to visualize the evolutionary relationship among enterococcal species and if they segregate based on host environment. We also review the genomic basis of antimicrobial resistance in Enterococcal species across the One Health Continuum.

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Vagococcus allomyrinae sp. nov. and Enterococcus larvae sp. nov., isolated from larvae of Allomyrina dichotoma

Cited by 11 publications

References 37 publications

Vagococcus luciliae sp. nov., isolated from the common green bottle fly Lucilia sericata

Vagococcus luciliae sp. nov., isolated from the common green bottle fly Lucilia sericata

Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat

Enterococci as a One Health indicator of antimicrobial resistance

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