Vagococcus zengguangii sp. nov., isolated from yak faeces

Ge, Yajun; Jin, Dong; Lai, Xin‐He; Yang, Jing; Lu, Shan; Huang, Ying; Zheng, Han; Zhang, Xiaoyan; Xu, Jianguo

doi:10.1007/s12275-021-0406-3

Cited by 2 publications

(2 citation statements)

References 47 publications

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“…Our laboratory has a long term interest in investigating the intestine (via fecal samples) microbiome diversity of wild animals such as Equus kiang (Huang et al, 2019), Marmota himalayana (Liu et al, 2015), Pantholops hodgsonii (Bai et al, 2016), and yak (Ge et al, 2021) to identify potentially pathogenic microbial species and dissect the process of disease transmission. Recently, bats have attracted global attention because of their zoonotic association with severe acute respiratory syndrome coronaviruses (SARS-CoV and SARS-CoV-2) (Hu et al, 2021).…”

Section: Purpose Of Isolationmentioning

confidence: 99%

Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces

et al. 2022

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Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170 T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20′54″N, 106°49′20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09′10″N, 102°04′39″E, October 2013) of South China, respectively. Optimal growth is obtained at 25–28°C (range, 4–32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0–10.0) in the presence of 0.5–1.0% NaCl (w/v) (range, 0–15% NaCl [w/v]). The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium , most closely related to B. aurantiacum NCDO 739 T (16S rRNA similarity, both 98.5%; dDDH, 46.7–46.8%; ANI, 91.9–92.1%). Strain HY170 T contained MK-8(H 2 ), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively. The meso-diaminopimelic acid ( meso -DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170 T . Anteiso -C 15:0 and anteiso -C 17:0 were the major fatty acids (> 10%) of strains HY170 T and HY001, with anteiso -C 17:1 A predominant in strain HY170 T but absent in strain HY001. Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene. Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium , for which the name Brevibacterium zhoupengii sp. nov. is proposed (type strain HY170 T = CGMCC 1.18600 T = JCM 34230 T ). Supplemental material for this article may be found at 10.1007/s12275-022-2134-8.

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Section: Purpose Of Isolationmentioning

confidence: 99%

Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces

et al. 2022

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“…Vagococci have been detected in humans [3] and animals, such as seal, chicken, otter, marten, harbour porpoise, salmonid fish and snow finch [1,[4][5][6][7][8][9]. They have also been detected in livestock-associated environments [10][11][12] and foods containing animal products [3,[13][14][15]. The other sources of isolation have included grain [16], bioreactors [17] and insects [18,19].…”

Section: Introductionmentioning

confidence: 99%

Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat

Johansson

Jääskeläinen

Säde

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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During our studies on spoilage microbiomes of modified atmosphere packaged broiler meat, we isolated three strains (PNs007T, STAA11T and STAA25) of unknown identity. In this present polyphasic taxonomy study, including genome-based analyses, we discovered that these isolates represent two novel species belonging to the genus Vagococcus . In all phylogenetic analyses, PNs007T was positioned very close to Vagococcus fessus but both the average nucleotide identity (ANI; 89.5 %) and digital DNA–DNA hybridization (dDDH; 38.3 %) values distinguished it as a novel vagococcal species. STAA11T and STAA25 were genetically highly similar (16S rRNA, ANI and dDDH 100 %). The phylogenetic position of STAA11T was adjacent to but out of the cluster containing V. fessus , Vagococcus coleopterorum and PNs007T. According to the ANI (76.2–76.4 %) and dDDH (<22.6 %) values it also represented a novel vagococcal species. Phenotypic characteristics and chemotaxonomic properties of both novel species were typical for vagococci and they contained C16 : 0 (25.5–30.1 %) and C18 : 1 ω9c (67.3–73.0 %) as the major cellular fatty acids. The streptomycin-resistant genotype of STAA11T and STAA25 allowing the growth on streptomycin thallous acetate actidione medium was considered to result from a modification in codon 104 of the rpsL gene leading to P104A substitution. The ability of STAA11T and STAA25 to produce ammonia from arginine separated them from PNs007T, which did not show arginine deiminase activity. We propose the names Vagococcus proximus sp. nov. (type strain PNs007T=DSM 115185T=CCUG 76696T) and Vagococcus intermedius sp. nov. (type strain STAA11T=DSM 115183T=CCUG 76697T) for these novel species.

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Vagococcus zengguangii sp. nov., isolated from yak faeces

Cited by 2 publications

References 47 publications

Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces

Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces

Vagococcus proximus sp. nov. and Vagococcus intermedius sp. nov., originating from modified atmosphere packaged broiler meat

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