Validasi dari Spektrofotometri UV-Vis dan Kandungan Total Flavonoid Ekstrak Etanol dari Akar Alang-Alang (Imperata cylindrica) dan Daun Pegagan (Centella asiatica) Anita Puspa Widiyana

Abstract

Validation as a quality control for the content of chemical compounds from natural ingredients. One of the chemical constituents is the flavonoids which are found in the Imperatacylindrica roots and Centella asiatica leaves. This study aims to ensure the analysis method meets the requirements and determines the levels of flavonoids. The research stages included extraction, validation and determination of total flavonoid. Extraction was carried out by immersing dry simplicia in 96% ethanol solvent for 3x24 hours. The solvent is evaporated using a rotary evaporator until a thick extract is formed. Validation testing includes linearity, accuracy, precision, LOD and LOQ. Determinationof the total flavonoid was carried out by measuring the absorption at a maximum wavelength of 428.2 nm. The validation results includethe correlation coefficient (R) of 0.998, precision % RSD <2 %, %accuracy 99,53-97,98%, LOD 3.02ppm and LOQ 9.15 ppm. The total flavonoid of the ethanol extract of Imperata cylindrica roots was 36.39 ± 0.08 mg/g QE and Centella asiatica leaves was 102.10 ± 0.08 mg/g QE. The conclusion is that the method used met the validation requirements and the total flavonoid content of the ethanol extract of Centella asiatica leaves was higher than Imperata cylindrica roots