Validated HPTLC fingerprinting and antioxidant activity evaluation of twenty-seven Romanian red wines

Hosu, Anamaria; Danciu, Virgil; Cimpoiu, Claudia

doi:10.1016/j.jfca.2015.02.004

Cited by 24 publications

(12 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concerning the variability observed among the samples, it was expected and attributed to different conditions of plant development such as geographic origin (collection), climate, and soil conditions. [ 31 32 ] Thus, the procedure's ability to detect the differences was achieved and proved be able to support the establishment of standard fingerprint to assure the chemical and biological properties of such raw material and their extractives.…”

Section: Resultsmentioning

confidence: 99%

Chromatographic and spectrophotometric analysis of phenolic compounds from fruits of Libidibia ferrea Martius

Ferreira¹,

Fernandes²,

Silva³

et al. 2016

Phcog Mag

View full text Add to dashboard Cite

Background:Libidibia ferrea (Mart. ex Tul.) L.P. Queiroz (Fabaceae) is a tree which is native to Brazil, widely known as “Jucá,” where its herbal derivatives are used in folk medicine with several therapeutic properties. The constituents, which have already been described in the fruit, are mainly hydrolysable tannins (gallic acid [GA] and ellagic acid [EA]).Objective:The aim of this study was to investigate the phenolic variability in the fruit of L. ferrea by ultraviolet/visible (UV/VIS) and chromatographic methods (high-performance liquid chromatography [HPLC]/high-performance thin layer chromatography [HPTLC]).Materials and Methods:Several samples were collected from different regions of Brazil and the qualitative (fingerprints by HPTLC and HPLC) and quantitative analysis (UV/VIS and HPLC) of polyphenols were performed.Results:The HPTLC and HPLC profiles allowed separation and identification of both major analytical markers: EA and GA. The chemical profiles were similar in a number of spots or peaks for the samples, but some differences could be observed in the intensity or area of the analytical markers for HPTLC or HPLC, respectively. Regarding the quantitative analysis, the polyphenolic content by UV/VIS ranged from 13.99 to 37.86 g% expressed as GA or from 10.75 to 29.09 g% expressed as EA. The contents of EA and GA by liquid chromatography-reversed phase (LC-RP) method ranged from 0.57 to 2.68 g% and from 0.54 to 3.23 g%, respectively.Conclusion:The chemical profiles obtained by HPTLC or HPLC, as well as the quantitative analysis by spectrophotometry or LC-RP method, were suitable for discrimination of each herbal sample and can be used as tools for the comparative analysis of the fruits from L. ferrea.SUMMARY The polyphenols of fruits of Libidibia ferrea can be quantified by UV/VIS and HPLCThe HPLC method was able to detect the gallic and ellagic acids in several samples of fruits of Libidibia ferreaThe phenolic profiles of fruits from Libidibia ferrea by HPTLC and HPLC were reproductible. Abbreviations used: HPTLC: high performance thin layer chromatography, HPLC: high performance liquid chromatography, UV-Vis: spectrophotometry

show abstract

Section: Resultsmentioning

confidence: 99%

Chromatographic and spectrophotometric analysis of phenolic compounds from fruits of Libidibia ferrea Martius

Ferreira¹,

Fernandes²,

Silva³

et al. 2016

Phcog Mag

View full text Add to dashboard Cite

show abstract

“…Consequently, the areas corresponding to the reduction of the DPPH stain on the plate depend on the antiradical activity of the compound, and this activity is expressed as the equivalent of a reference: the pure standard of the compound. Anthocyanins and DPPH absorb at similar wavelengths, as the optimal absorbance measurement wavelength of major anthocyanins is 520 nm (Cretu et al, 2013;Cretu and Morlock, 2014;Hosu et al, 2015), but transmittance scanning at 517 nm overcomes this problem as it measures the area bleached by the compound of interest on the plate. The greater is the bleached spot, the larger is the peak area obtained in AU (Fig.…”

Section: Dpph Testmentioning

confidence: 99%

“…Thin layer densitometry has been shown to be a reliable and efficient tool for the quantitative evaluation of the free radical scavenging activity of antioxidants by measuring the inhibition of 2,2-diphenyl-1-picrylhydrazyl, DPPH (Abourashed, 2005). Bleaching of the oxidised DPPH purple colour caused by antioxidants on a thin layer chromatography (TLC) plate can be measured with computerised image processing (Olech et al, 2012) to allow the simultaneous quantitative evaluation of the antioxidant activity of different samples (Hosu et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Identification and quantification of phenolic compounds responsible for the antioxidant activity of sweet potatoes with different flesh colours using high performance thin layer chromatography (HPTLC)

Lebot

Michalet

Legendre

2016

Journal of Food Composition and Analysis

View full text Add to dashboard Cite

“…The most popular methods are based on ultraviolet-visible (UV-Vis) spectrometry, but chromatographic methods such as high-performance liquid chromatography (HPLC) or (high-performance) thin-layer chromatography (HP)TLC have also been used [16–18]. Several types of free radicals have been employed to estimate the scavenging capacity, but the ABTS +∙ decolorization assay has many advantages, namely, being more sensitive than the DPPH assay [19]; the reagent can be solubilized in both aqueous and organic media and is not affected by ionic strength, enables the measurement of both hydrophilic and lipophilic antioxidants, and so forth [15].…”

Section: Introductionmentioning

confidence: 99%

Study of the Antioxidant Property Variation of Cornelian Cherry Fruits during Storage Using HPTLC and Spectrophotometric Assays

Hosu

Cimpoiu

David

et al. 2016

Journal of Analytical Methods in Chemistry

Self Cite

View full text Add to dashboard Cite

Cornus species fruits are well known as a rich source of antioxidant compounds responsible for their diverse health benefits. The present study aims to investigate the variation of the total antioxidant capacity of Cornelian cherry (Cornus mas L.) fruits during storage, using high-performance thin-layer chromatography (HPTLC) and two spectrophotometric assays based on different mechanisms: the 2,2-azinobis(3-ethylbenzothiazolyne-6-sulphonic acid) radical cation (ABTS+∙) assay and the ferric reducing antioxidant power (FRAP) assay. The fruit extract was stored at room temperature (22°C) for 19 days. No major differences in the total antioxidant capacity were observed during this period, indicating that storage does not have any deleterious effect on the antioxidant properties of the investigated fruits extract. The antioxidant capacity varied between 12.91 and 12.83 µmol Trolox/g fruit as determined by the HPTLC method and from 36.13 to 33.93 µmol Trolox/g fruit as determined by the ABTS assay.

show abstract

Validated HPTLC fingerprinting and antioxidant activity evaluation of twenty-seven Romanian red wines

Cited by 24 publications

References 22 publications

Chromatographic and spectrophotometric analysis of phenolic compounds from fruits of Libidibia ferrea Martius

Chromatographic and spectrophotometric analysis of phenolic compounds from fruits of Libidibia ferrea Martius

Identification and quantification of phenolic compounds responsible for the antioxidant activity of sweet potatoes with different flesh colours using high performance thin layer chromatography (HPTLC)

Study of the Antioxidant Property Variation of Cornelian Cherry Fruits during Storage Using HPTLC and Spectrophotometric Assays

Contact Info

Product

Resources

About