Validated HPTLC Method for Simultaneous Estimation of Atenolol and Aspirin in Bulk Drug and Formulation

Abstract

This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Atenolol and Aspirin as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F 254 as the stationary phase and the solvent system consisted of n-butanol : water : acetic acid (8 : 2 : 0.2 v/v/v). Densitometric evaluation of the separated zones was performed at 235 nm. The two drugs were satisfactorily resolved with R F values 0.23 ± 0.02 and 0.72 ± 0.02 for Atenolol and Aspirin, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (100-600 ng/spot for Atenolol and Aspirin), precision (intraday % RSD was 0.48-1.03 and interday % RSD was 0.68-1.14 for Atenolol, and intraday % RSD was 0.61-1.03 and interday % RSD was 0.69-1.04 for Aspirin), accuracy (100.17 ± 0.32 for Atenolol and 99.73 ± 0.51 for Aspirin), and specificity in accordance with ICH guidelines.

Comparative study and multiple linear regression analysis for assessment of chromatographic behavior of structurally related β‐blockers on different stationary phases

“…Derivatization with absyl chloride was required to improve densitometric detection of BISO and LAB in their tablets [9]. Many other articles analyzed studied drugs successfully as native and in combination with other β-blocking agents [10,11] and with other cardiovascular agents [12][13][14][15][16][17][18][19]. Enantiomers of both ATENO and PROP were resolved and quantified using three different chiral selectors as impregnating reagents [20].…”

Development and validation of stability indicating RP-HPLC method for determination of aspirin and pantoprazole sodium in synthetic mixture stability indicating HPLC method

“…1 Currently it is having legal status in Indian Pharmacopeia (IP), British Pharmacopeia (BP) and United State Pharmacopeia (USP). Extensive literature search highlights majorly UV spectroscopy, 2-5 HPLC [6][7][8] and HPTLC [9][10][11] as analytical methods for analysis of Aspirin from bulk and pharmaceutical dosage form.…”

Simultaneous Determination of Atenolol and Amlodipine Using Second Derivative Spectroscopy

“…Amlodipine is used for treatment of hypertension and angina pectoris (Ayad et al, 2012). Amlodipine acting by relaxation of the smooth muscle in arterial wall, decreasing total peripheral resistance and thus reducing blood pressure (Bhusari and Dhaneshwar, 2012). Atenolol chemically is 4-(2-hydroxy-3-isopropylaminopropoxy)-phenyl acetamide.…”