Validated Method for the Determination of Piroxicam by Capillary Zone Electrophoresis and Its Application to Tablets

Abstract

Simple and rapid capillary zone electrophoretic method was developed and validated in this study for the determination of piroxicam in tablets. The separation of piroxicam was conducted in a fused-silica capillary by using 10 mM borate buffer (pH 9.0) containing 10% (v/v) methanol as background electrolyte. The optimum conditions determined were 25 kV for separation voltage and 1 s for injection time. Analysis was carried out with UV detection at 204 nm. Naproxen sodium was used as an internal standard. The method was linear over the range of 0.23–28.79 µg/mL. The accuracy and precision were found to be satisfied within the acceptable limits (<2%). The LOD and LOQ were found to be 0.07 and 0.19 µg/mL, respectively. The method described here was applied to tablet dosage forms and the content of a tablet was found in the limits of USP-24 suggestions. To compare the results of capillary electrophoretic method, UV spectrophotometric method was developed and the difference between two methods was found to be insignificant. The capillary zone electrophoretic method developed in this study is rapid, simple, and suitable for routine analysis of piroxicam in pharmaceutical tablets.

Attenuated total reflection‐Fourier transform infrared spectroscopy (ATR‐FTIR) for quantification of piroxicam in tablet dosage from as a green analytical method

“…[1,2] formulations, such as spectrophotometry, highperformance liquid chromatography, voltammetry, differential pulse voltammetry, and capillary electrophoresis. [3][4][5][6][7][8][9][10] The disadvantages of these techniques are the need for large quantities of organic solvents, long analysis times and complex sample preparation steps. Organic solvents such as methanol, acetonitrile, trifluoroacetic acid, and borate buffer are not only very expensive, but also cause environmental damage.…”

Sensitive Voltammetric Method for Piroxicam Determination in Pharmaceutical, Urine and Tap Water Samples Using an Anodically Pretreated Boron-Doped Diamond Electrode

“…Several analytical methods for assaying PRX in pharmaceutical formulations and human body fluids have been described in the literature [6][7][8][9][10][11][12][13][14]. Most of these methods use chromatographic and spectrophotometric techniques in which, commonly, organic toxic solvents are used, generating high amounts of waste, the analytical frequency is low and requires prior separation steps and the analytical process is tedious.…”

Determinación de las constantes de equilibrio de inclusión de los complejos de Isoxican con 2- Hidroxipropil-β-Ciclodextrina

“…No obstante, pese a las ventajas que ofrece el uso de isoxicam, este posee una solubilidad mucho menor comparada a la de otros AINEs, por lo cual actualmente no se comercializa para uso farmacéutico [1][2][3][4][5][6][7][8][9][10] . Figura 1.2.…”

“…A Dios y a mis padres: Ricardo y Mª Guadalupe por haber confiado en mí, por todo el amor y apoyo que siempre me han brindado Ante la desventaja ya mencionada, el uso de agentes complejantes como las ciclodextrinas, en particular la 2-Hidroxipropil-β-ciclodextrina (por ser más soluble respecto a otras ciclodextrinas) para la formación de complejos de inclusión, ofrece una alternativa para aumentar la solubilidad de este tipo de fármacos y así fomentar su uso [1][2][3][4][5][6][7][8] .…”

“…Dar a conocer la existencia de complejos de inclusión de isoxicam con ciclodextrinas, así como algunas de sus propiedades como: constantes de equilibrio, estequiometría, ángulo de rotación, conductividad, entre otras, puede ser de gran utilidad para que en un futuro se convierta en un analgésico competitivo como lo son piroxicam, tenoxicam, meloxicam, por mencionar algunos [5][6][7][8][9][10] .…”