Validated specific HPLC method for determination of zidovudine during stability studies

Ashenafi, Dunge; Sharda, Nishi; Singh, Baljinder; Singh, Saranjit

doi:10.1016/j.jpba.2004.09.013

Cited by 25 publications

(14 citation statements)

References 16 publications

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“…Several methods have been discovered for the quantitative determination of zidovudine including chromatog- raphy [9][10][11][12][13][14][15], fluorescence polarization immunoassay [16], using diamond paste based immunosensor [17], fluorescence spectroscopy [18] and voltammetry [19][20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Determination of the Antiretroviral Drug Zidovudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Castro¹,

Aucélio²,

Rey³

et al. 2011

AJAC

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This paper describes a stripping method for the determination of zidovudine at the submicromolar concentration levels. This method is based on the controlled adsorptive accumulation of zidovudine at the thin-film mercury electrode, followed by a linear-sweep stripping voltammetry measurement of the surface species. Optimal experimental conditions include a NaOH solution of 2.0 × 10 -3 mol·L -1 (supporting electrolyte), an accumulation potential of -0.30 V and a scan rate of 100 mV·s -1 . The response of zidovudine is linear over the concentration range 0.01 -0.08 ppm. After an accumulation time of 5 minutes, the detection limit was found to be 0.67 ppb (2.5 × 10 -9 mol·L -1 ). More convenient methods to measure zidovudine concentration in the presence of the didanosine, acyclovir, nevirapine, lamivudine, and efavirenz, were also investigated. The presence of zidovudine together with ATP or ssDNA demonstrates the utility of this method.

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Section: Introductionmentioning

confidence: 99%

Determination of the Antiretroviral Drug Zidovudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Castro¹,

Aucélio²,

Rey³

et al. 2011

AJAC

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“…The drug is a rst nucleoside reverse transcriptase inhibitor, which was approved by the United States Food and Drug Administration (USFDA) in 1987 for the treatment of acquired immunodeciency syndrome (AIDS). 6 In our previous reports, 7,8 we identied 5-methylpyrimidine-2,4(1H,3H)-dione (thymine) as the major degradation product of the drug under hydrolytic and photolytic conditions. In a recent paper, Devrukhakar et al 9 identied 1-5-(hydromethyl)teterahydrofuran-2-yl-5-methylpyrimidine-2,4(1H,3H)-dione, along with thymine as the degradation products of ZDV under acid conditions.…”

Section: Introductionmentioning

confidence: 98%

Stability behaviour of antiretroviral drugs and their combinations. 6: evidence of formation of potentially toxic degradation products of zidovudine under hydrolytic and photolytic conditions

et al. 2017

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This study explored the comprehensive degradation behaviour of zidovudine (ZDV) under solution and solid stress conditions. In total, nine degradation products were detected by high performance liquid chromatography (HPLC). The same were tentatively characterized with the help of high resolution and multistage mass spectrometry. Among them, five degradation products were also enriched and isolated with the help of semi-preparative HPLC and subjected to 1D and 2D nuclear magnetic resonance and/or infrared spectrometric studies to confirm their structures. The characterization of all the degradation products helped in outlining the comprehensive degradation pathway of ZDV. A significant finding was the formation of 3 0-amino-3 0-deoxythymidine (AMT) upon base hydrolysis as well as photolysis of the drug. This product is a known catabolite of the drug with a high degree of toxicity. Also, a few other degradation products formed during the study were predicted to have potential toxicity.

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“…[1][2][3][4] In addition, the standard addition approach and peak purity testing may be used to validate selectivity of the analytical method for an analyte when matrix cannot be replicated in standards or impurity and degradant standards are not available. [5][6][7][8] The standard addition method is recommended for the confirmation of the presence of matrix effect (proportional effect) by comparison of slopes between the external calibration and standard addition curve. [8,9] In addition, it can be applied as the calibration approach over an external calibration method when matrix effects are present.…”

Section: Introductionmentioning

confidence: 99%

Selectivity Investigation and Liquid Chromatographic Method for the Analysis of Nicotine in Tobacco Extracts

Delvadia

Karnes

2013

Journal of Liquid Chromatography & Related Technologies

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& A simple and selective high performance liquid chromatographic (HPLC) method for the analysis of nicotine in a Hanks' balanced salts extract of smokeless tobacco components is presented here. An HPLC method was modified and applied for the analysis of nicotine in samples obtained from a biorelevant in vitro release system for permeation testing of nicotine from a commercially available smokeless tobacco product. The selectivity for the analysis of nicotine in vitro samples was investigated as a part of the method validation by the standard addition method, peak purity testing, and peak trapping followed by mass spectrometry (MS) identification of a co-eluant which was observed. The co-eluting compound was attributable to a leachable substance from the neoprene O-ring used in the apparatus. The mass spectrum (molecular ion with m=z 353.8 % 354) for this substance was consistent with the molecular weight of the plasticizer, alkyl alkylether diester adipate (molecular weight 353), commonly used in manufacturing rubber. Selectivity was shown without chromatographic resolution of the co-eluting substance by selection of an appropriate wavelength range where the co-eluant did not absorb UV light.

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Validated specific HPLC method for determination of zidovudine during stability studies

Cited by 25 publications

References 16 publications

Determination of the Antiretroviral Drug Zidovudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Determination of the Antiretroviral Drug Zidovudine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Stability behaviour of antiretroviral drugs and their combinations. 6: evidence of formation of potentially toxic degradation products of zidovudine under hydrolytic and photolytic conditions

Selectivity Investigation and Liquid Chromatographic Method for the Analysis of Nicotine in Tobacco Extracts

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