Validated Spectrophotometric method for analysis of Dipyridamole and Lamivudine using eosin Y

Barghash, Sona; El-Razeq, Sawsan A. Abd; Awady, Mohamed E. El; Belal, Fathalla

doi:10.21608/aijpms.2021.52761.1016

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…Overall, due to its impressive benefits, DIP determination in pharmaceuticals and various biological samples became really important. There are numerous analytical methods proposed for this drug quantification which encompass more conventional techniques, such as HPLC [6][7][8][9][10] and spectrophotometry [11,12]. Despite their advantages, these methods have also shown important limitations, such as the need for time-consuming preliminary procedures, such as extraction and concentration in organic solvents, that imply large volumes of samples and reagents, expensive equipment, and the necessity of specialized personnel, in the case of chromatographic methods.…”

Section: Introductionmentioning

confidence: 99%

Determination of Dipyridamole Using a MIP-Modified Disposable Pencil Graphite Electrode

et al. 2023

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A new method for the determination of the antiplatelet drug dipyridamole (DIP) in pharmaceuticals using a molecularly imprinted polymer (MIP)-modified pencil graphite electrode (PGE) is proposed. The modified electrode was prepared simply and rapidly by electropolymerization of caffeic acid (CA) in the presence of DIP and subsequent DIP extraction with ethanol, resulting in a cost-effective, eco-friendly disposable modified electrode (MIP_PGE). Several working conditions (monomer and template concentration, number of voltametric cycles, scan rate extraction time, and solvent) for the MIP_PGE preparation were optimized. The differential pulse voltammetric (DPV) oxidation signal of DIP obtained at MIP_PGE was 28% higher than that recorded at bare PGE. Cyclic voltammetry emphasized DIP irreversible, pH-dependent, diffusion-controlled oxidation at MIP_PGE. Differential pulse and adsorptive stripping voltammetry at MIP_PGE in phosphate buffer solution pH = 7.00 were applied for the drug quantitative determination in the range of 1.00 × 10−7–1.00 × 10−5 and 1.00 × 10−8–5.00 × 10−7 mol/L DIP, respectively. The obtained limits of detection were at the tens nanomolar level.

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Section: Introductionmentioning

confidence: 99%

Determination of Dipyridamole Using a MIP-Modified Disposable Pencil Graphite Electrode

et al. 2023

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“…[23] Complexes formed between the ion-pairing agent eosin Y and numerous drugs for spectrophotometric or spectrofluorimetric investigation have been published in the literature. [24][25][26][27][28][29] Because many extraction processes utilize halogenated solvents, which are hazardous to humans and the surrounding environment, these methods have several disadvantages, including reduced sensitivity and decreased simplicity. In this work, eosin is used for the determination of GTF and the formation of the binary complex without further extraction procedures for the resulting complex.…”

Section: Introductionmentioning

confidence: 99%

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Gammal

El‐Wasseef

El‐Ashry

et al. 2022

J Chinese Chemical Soc

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Multiple sensitive eco-friendly analytical techniques were applied for the assessment of two important fourth-generation fluoroquinolones namely, gemifloxacin (GFX), and gatifloxacin (GTF). GFX, which is utilized as an essential adjunct therapy for the management of pneumonia in SARS-CoV-2 was determined by green indicating micellar liquid chromatographic in authentic powder and dosage forms. The separation was performed by using a reversed-phase C 18 column; the micellar mobile phase comprised of 12.5% npropanol, 0.15 M sodium lauryl sulfate, and 0.3% triethylamine in 0.02 M Ophosphoric acid adjusted to pH 3.0 and pumped at 1 ml/min flow rate and detected at 266 nm by UV detection. The method was linear over the range of 4.0-90 μg.ml À1 with a recovery percentage of 99.81 ± 0.69. The acidic degradation behavior of GFX along with the kinetic investigation was evaluated as recommended by ICH-stress conditions. The separation of the degradation product was performed in good elution time (less than 8 min), and the suggested technique has a good reproducibility (R.S.D. less than 1.0%) and provides an excellent resolution (R s = 4.5) between GFX and its degradation product. Concerning the second drug, GTF, simple, extremely sensitive, and accurate spectrofluorimetric and spectrophotometric techniques were adopted for the determination of the drug in its pharmaceutical preparations. The suggested approaches utilize the concept of complex formation with eosin Y in an aqueous buffered solution. Regarding the spectrophotometric method (method I), the binary complex has a maximum absorption at 544 nm, while for spectrofluorimetric determination (method II) the formed complex was recorded at λ em 491 nm after excitation at 286 nm. For both approaches, variables affecting the formation of the binary complexes were extensively investigated and optimized. The suggested techniques were shown to be rectilinear across the concentration range of 0.5-8 and 0.05-1 μg.ml À1 , respectively, with recovery percentages of 100.34 ± 0.85 and 99.90 ± 0.81 for method (I) and method (II), respectively. In compliance with ICH guidelines, the analytical performance of

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Curcumin-Based Molecularly Imprinted Polymer Electropolymerized on Single-Use Graphite Electrode for Dipyridamole Analysis

Preda,

Radu,

Iorgulescu

et al. 2024

Molecules

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A new molecularly imprinted polymer (MIP)-based disposable electrochemical sensor for dipyridamole (DIP) determination was obtained. The sensor was rapidly prepared by potentiodynamic electrochemical polymerization on a pencil graphite electrode (PGE) using curcumin (CUR) as a functional monomer and DIP as a template molecule. After the optimization of the conditions (pH, monomer–template ratio, scan rate, number of cyclic voltammetric cycles applied in the electro-polymerization process and extraction time of the template molecule) for MIP formation, DIP voltammetric behavior at the modified electrode (MIP_PGE) was investigated. DIP oxidation took place in a pH-dependent, irreversible mixed diffusion-adsorption controlled process. Differential pulse voltammetry (DPV) and adsorptive stripping differential pulse voltammetry (AdSDPV) were used to quantify DIP from pharmaceutical and tap water samples. Under optimized conditions (Britton–Robinson buffer at pH = 3.29), the obtained linear ranges were 5.00 × 10−8–1.00 × 10−5 mol/L and 5.00 × 10−9–1.00 × 10−7 mol/L DIP for DPV and AdSDPV, respectively. The limits of detection of the methods were 1.47 × 10−8 mol/L for DPV and 3.96 × 10−9 mol/L DIP for AdSDPV.

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Validated Spectrophotometric method for analysis of Dipyridamole and Lamivudine using eosin Y

Cited by 3 publications

References 4 publications

Determination of Dipyridamole Using a MIP-Modified Disposable Pencil Graphite Electrode

Determination of Dipyridamole Using a MIP-Modified Disposable Pencil Graphite Electrode

Multiple sensitive eco‐friendly analytical approaches for the determination of two fourth‐generation fluoroquinolones: Application to stability study and content uniformity testing

Curcumin-Based Molecularly Imprinted Polymer Electropolymerized on Single-Use Graphite Electrode for Dipyridamole Analysis

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