Validated Spectrophotometric Method to Assay of B&lt;sub&gt;6&lt;/sub&gt; and B&lt;sub&gt;3&lt;/sub&gt; Vitamins in Pharmaceutical Forms Using Potassium Iodide and Potassium Iodate

Khateeb, Mouhammed; Elias, Basheer; Rahal, Fatema Al

doi:10.18052/www.scipress.com/ilcpa.60.113

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…Iodide ions transform to free iodine in an acidic medium of CLN.HCl solution, the acidity comes from HCl then free iodine reacts with an excess of iodide ions to form yellow complex from tri iodide (I 3-) (Figure 2) [20][21].…”

Section: Resultsmentioning

confidence: 99%

Determination of CLINDAMYCIN HCl in Capsules by New, Validated, Simple and Green Kinetic Spectrometric Method

Affas¹,

Sakur

2021

Preprint

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Background: simple, sensitive, free of organic solvents, kinetic spectrophotometric method has been developed for the determination of Clindamycin Hydrochloride, both in pure form and Capsules. Method used is based on reaction of Clindamycin with potassium iodide and potassium iodate in aqueous medium at (25 ±2 °c) to produce yellow colored tri iodide ions (I3-). the reaction is followed spectrophotometrically by measuring the absorbance at 350 nm wavelength during 40 minutes. Results: the effects of analytical parameters on reported kinetic methods were investigated. Under the optimized conditions, the initial rate and fixed time (at 10 min) methods were used for constructing the calibration graphs. The graphs were linear in concentration ranges 1-20 μg.ml-1 with limit of detection of 0.12 and 0.22 μg ml-1for the initial rate and fixed time methods, respectively. The results were satisfactory and the analytical performance for both methods was validated. Conclusion: The proposed methods have been applied to determine the components in capsules with an average recovery of 98.25% to 102.00% and the results are in good agreement with those found by the reference method.

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Section: Resultsmentioning

confidence: 99%

Determination of CLINDAMYCIN HCl in Capsules by New, Validated, Simple and Green Kinetic Spectrometric Method

Affas¹,

Sakur

2021

Preprint

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“…The ideal conditions for the improvement of the method were recognized by changing the parameters one at a time and keeping the others fixed and observing the influence produced on the absorbance of the coloured products. [ 19 ]…”

Section: Resultsmentioning

confidence: 99%

“…The rate data of reaction would follow a pseudo order rate constant and submitted the following rate equation: [ 19 ] …”

Section: Resultsmentioning

confidence: 99%

“…The rate data of reaction would follow a pseudo order rate constant and submitted the following rate equation: [19] where: ν: reaction rate, A: absorbance., ∆A = At 2 − At 1 , t is the measuring time, ∆t = t2 − t1, K is the pseudo order rate constant, c is the concentration of (CLI) mol/L and n is the order of the reaction. [21] A calibration curve was constructed by plotting the logarithm of the initial rate of reaction (log ν) against the logarithm of (CLN.HCl) concentration (log c), which…”

Section: Initial Rate Methodsmentioning

confidence: 99%

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Validated green spectrophotometric kinetic method for determination of Clindamycin Hydrochloride in capsules

Affas¹,

Sakur

2021

BMC Chemistry

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Background simple, sensitive, free of organic solvents, kinetic spectrophotometric method has been developed for the determination of Clindamycin Hydrochloride, both in pure form and Capsules. Method is based on reaction of Clindamycin with potassium iodide and potassium iodate in an aqueous medium at (25 ± 2 °C) to produce yellow-coloured tri iodide ions (I3−). The reaction is followed spectrophotometrically by measuring the absorbance at wavelength 350 nm during 40 min. Results the effects of analytical parameters on reported kinetic methods were investigated. Under the optimized conditions, the initial rate and fixed time (at 10 min) methods were used for constructing the calibration graphs. The graphs were linear in concentration ranges 1–20 μg ml−1 with limit of detection of 0.12 and 0.22 μg ml−1for the initial rate and fixed time methods, respectively. The results were satisfactory and the analytical performance for both methods was validated. Conclusion The proposed methods have been applied to determine the components in capsules with an average recovery of 98.25–102.00% and the results are in good agreement with those found by the reference method.

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“…It is vital to develop sensitive and selective methods for the quick determination and monitoring of VB6 in various matrices, such as food and pharmaceuticals, in light of its significance and potential negative effects. For the purpose of determining VB6, a number of analytical techniques, including liquid chromatography [8], gas chromatography-mass spectrometry [9], chemiluminescence [10], capillary zone electrophoresis [11] and spectrophotometry [12] have been described in the literature. Many of these analytical techniques require tedious and time-consuming procedures and involve highly sophisticated instrumentation and toxic organic reagents [5,13,14].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical determination of vitamin B6 in pharmaceutical and energy drink samples

Tesfaye¹,

Tessema²,

Negash³

2023

J. Electrochem. Sci. Eng.

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A simple and low-cost electrochemical sensor based on poly(phenylalanine) and functionnalized multi-walled carbon nanotubes (F-MWCNTs) modified glassy carbon electrode (GCE) was developed for the determination of vitamin B6 (VB6). The surface morphology of modified glassy carbon electrodes was investigated with scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The electrocatalytic activities of the bare and modified electrodes were investigated in the presence of ferri-ferrocyanide redox couple using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The exchange current density (jo = 2462 µA cm-2) and electron transfer rate constant (ko = 0.002 cm s−1) were calculated using 5 mM K3[Fe(CN)6]. The electrochemical activity of poly(phenylalanine)/F-MWCNT/GCE towards VB6 oxidation was investigated using CV. Parameters including the number of electrons transferred (n = 2), number of protons transferred (H+ = 2), electron transfer coefficient (α = 0.51) and surface concentration of VB6 (G = 0.24 nmol cm−2) were calculated. At the optimal experimental conditions, the oxidation peak current of VB6 measured by square wave voltammetry (SWV) was found proportional to its concentration in two linear ranges of 0.5 to 20 µM and 20 to 200 µM with a low detection limit (LOD) of 0.038 µM and limit of quantification (LOQ) of 0.125 µM. Finally, the sensor was successfully used to determine VB6 in soft drink and pharmaceutical formulation samples.

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Validated Spectrophotometric Method to Assay of B<sub>6</sub> and B<sub>3</sub> Vitamins in Pharmaceutical Forms Using Potassium Iodide and Potassium Iodate

Cited by 9 publications

References 8 publications

Determination of CLINDAMYCIN HCl in Capsules by New, Validated, Simple and Green Kinetic Spectrometric Method

Determination of CLINDAMYCIN HCl in Capsules by New, Validated, Simple and Green Kinetic Spectrometric Method

Validated green spectrophotometric kinetic method for determination of Clindamycin Hydrochloride in capsules

Electrochemical determination of vitamin B6 in pharmaceutical and energy drink samples

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